ID   P5CS1_ARATH             Reviewed;         717 AA.
AC   P54887;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-DEC-2008, entry version 69.
DE   RecName: Full=Delta-1-pyrroline-5-carboxylate synthetase A;
DE            Short=P5CS A;
DE   Includes:
DE     RecName: Full=Glutamate 5-kinase;
DE              Short=GK;
DE              EC=2.7.2.11;
DE     AltName: Full=Gamma-glutamyl kinase;
DE   Includes:
DE     RecName: Full=Gamma-glutamyl phosphate reductase;
DE              Short=GPR;
DE              EC=1.2.1.41;
DE     AltName: Full=Glutamate-5-semialdehyde dehydrogenase;
DE     AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase;
GN   Name=P5CSA; Synonyms=P5CS1; OrderedLocusNames=At2g39800;
GN   ORFNames=T5I7.10;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=96032537; PubMed=7556633; DOI=10.1016/0014-5793(95)00935-3;
RA   Savoure A., Jaoua S., Hua X.J., Ardiles W., van Montagu M.,
RA   Verbruggen N.;
RT   "Isolation, characterization, and chromosomal location of a gene
RT   encoding the delta 1-pyrroline-5-carboxylate synthetase in Arabidopsis
RT   thaliana.";
RL   FEBS Lett. 372:13-19(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9351242; DOI=10.1046/j.1365-313X.1997.00557.x;
RA   Strizhov N., Abraham E., Oekresz L., Blickling S., Zilberstein A.,
RA   Schell J., Koncz C., Szabados L.;
RT   "Differential expression of two P5CS genes controlling proline
RT   accumulation during salt-stress requires ABA and is regulated by ABA1,
RT   ABI1 and AXR2 in Arabidopsis.";
RL   Plant J. 12:557-569(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=95291335; PubMed=7773306;
RA   Yoshiba Y., Kiyosue T., Katagiri T., Ueda H., Mizoguchi T.,
RA   Yamaguchi-Shinozaki K., Wada K., Harada Y., Shinozaki K.;
RT   "Correlation between the induction of a gene for delta 1-pyrroline-5-
RT   carboxylate synthetase and the accumulation of proline in Arabidopsis
RT   thaliana under osmotic stress.";
RL   Plant J. 7:751-760(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- FUNCTION: P5CS plays a key role in proline biosynthesis, leading
CC       to osmoregulation in plants.
CC   -!- CATALYTIC ACTIVITY: ATP + L-glutamate = ADP + L-glutamate 5-
CC       phosphate.
CC   -!- CATALYTIC ACTIVITY: L-glutamate 5-semialdehyde + phosphate +
CC       NADP(+) = L-glutamyl 5-phosphate + NADPH.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-
CC       glutamate 5-semialdehyde from L-glutamate: step 1/2.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-
CC       glutamate 5-semialdehyde from L-glutamate: step 2/2.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences;
CC       Name=1;
CC         IsoId=P54887-1; Sequence=Displayed;
CC   -!- SIMILARITY: In the N-terminal section; belongs to the glutamate 5-
CC       kinase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the gamma-
CC       glutamyl phosphate reductase family.
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DR   EMBL; X89414; CAA61593.1; -; Genomic_DNA.
DR   EMBL; X86777; CAA60446.1; -; mRNA.
DR   EMBL; X87330; CAA60740.1; -; mRNA.
DR   EMBL; D32138; BAA06864.1; -; mRNA.
DR   EMBL; AC003000; AAB87129.1; -; Genomic_DNA.
DR   EMBL; AF424633; AAL11626.1; -; mRNA.
DR   EMBL; AY113046; AAM47354.1; -; mRNA.
DR   EMBL; AY150430; AAN12972.1; -; mRNA.
DR   PIR; S66637; S66637.
DR   PIR; T50685; T50685.
DR   RefSeq; NP_181510.1; -.
DR   UniGene; At.20482; -.
DR   HSSP; Q9WYC9; 1O20.
DR   PRIDE; P54887; -.
DR   GeneID; 818566; -.
DR   GenomeReviews; CT485783_GR; AT2G39800.
DR   KEGG; ath:AT2G39800; -.
DR   TAIR; At2g39800; -.
DR   GermOnline; AT2G39800; Arabidopsis thaliana.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0016020; C:membrane; IDA:TAIR.
DR   GO; GO:0004349; F:glutamate 5-kinase activity; IEA:InterPro.
DR   GO; GO:0004350; F:glutamate-5-semialdehyde dehydrogenase acti...; IEA:InterPro.
DR   GO; GO:0042538; P:hyperosmotic salinity response; IMP:TAIR.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006561; P:proline biosynthetic process; IEA:InterPro.
DR   GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
DR   GO; GO:0009414; P:response to water deprivation; IEP:TAIR.
DR   GO; GO:0048364; P:root development; IMP:TAIR.
DR   InterPro; IPR016163; Ald_DHase_C.
DR   InterPro; IPR016162; Ald_DHase_N.
DR   InterPro; IPR001048; Asp/Glu/Uridylate_kinase.
DR   InterPro; IPR000965; Gglut_pp_reduct.
DR   InterPro; IPR001057; Glu_5kinase.
DR   InterPro; IPR005766; P5_carboxy_syn.
DR   InterPro; IPR005715; ProB.
DR   Gene3D; G3DSA:3.40.1160.10; Aa_kinase; 1.
DR   Gene3D; G3DSA:3.40.309.10; Aldehyde_dehydrogenase_C; 1.
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   Pfam; PF00696; AA_kinase; 1.
DR   PIRSF; PIRSF036429; P5C_syn; 1.
DR   PRINTS; PR00474; GLU5KINASE.
DR   TIGRFAMs; TIGR01092; P5CS; 1.
DR   TIGRFAMs; TIGR00407; proA; 1.
DR   TIGRFAMs; TIGR01027; proB; 1.
DR   PROSITE; PS00902; GLUTAMATE_5_KINASE; 1.
DR   PROSITE; PS01223; PROA; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Amino-acid biosynthesis; Complete proteome;
KW   Kinase; Multifunctional enzyme; NADP; Oxidoreductase;
KW   Proline biosynthesis; Transferase.
FT   CHAIN         1    717       Delta-1-pyrroline-5-carboxylate
FT                                synthetase A.
FT                                /FTId=PRO_0000109772.
FT   REGION        1    296       Glutamate 5-kinase.
FT   REGION      297    717       Gamma-glutamyl phosphate reductase.
FT   CONFLICT     42     42       C -> F (in Ref. 3; BAA06864).
FT   CONFLICT     54     54       E -> K (in Ref. 3; BAA06864).
FT   CONFLICT    381    381       D -> E (in Ref. 3; BAA06864).
FT   CONFLICT    467    467       L -> F (in Ref. 3; BAA06864).
FT   CONFLICT    676    676       H -> Y (in Ref. 3; BAA06864).
SQ   SEQUENCE   717 AA;  77702 MW;  DF2B296362351A78 CRC64;
     MEELDRSRAF ARDVKRIVVK VGTAVVTGKG GRLALGRLGA LCEQLAELNS DGFEVILVSS
     GAVGLGRQRL RYRQLVNSSF ADLQKPQTEL DGKACAGVGQ SSLMAYYETM FDQLDVTAAQ
     LLVNDSSFRD KDFRKQLNET VKSMLDLRVI PIFNENDAIS TRRAPYQDSS GIFWDNDSLA
     ALLALELKAD LLILLSDVEG LYTGPPSDPN SKLIHTFVKE KHQDEITFGD KSRLGRGGMT
     AKVKAAVNAA YAGIPVIITS GYSAENIDKV LRGLRVGTLF HQDARLWAPI TDSNARDMAV
     AARESSRKLQ ALSSEDRKKI LLDIADALEA NVTTIKAENE LDVASAQEAG LEESMVARLV
     MTPGKISSLA ASVRKLADME DPIGRVLKKT EVADGLVLEK TSSPLGVLLI VFESRPDALV
     QIASLAIRSG NGLLLKGGKE ARRSNAILHK VITDAIPETV GGKLIGLVTS REEIPDLLKL
     DDVIDLVIPR GSNKLVTQIK NTTKIPVLGH ADGICHVYVD KACDTDMAKR IVSDAKLDYP
     AACNAMETLL VHKDLEQNAV LNELIFALQS NGVTLYGGPR ASKILNIPEA RSFNHEYCAK
     ACTVEVVEDV YGAIDHIHRH GSAHTDCIVT EDHEVAELFL RQVDSAAVFH NASTRFSDGF
     RFGLGAEVGV STGRIHARGP VGVEGLLTTR WIMRGKGQVV DGDNGIVYTH QDIPIQA
//