ID   MAO1_BACSU              Reviewed;         439 AA.
AC   P54572;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-NOV-2008, entry version 69.
DE   RecName: Full=Probable NAD-dependent malic enzyme 1;
DE            Short=NAD-ME 1;
DE            EC=1.1.1.38;
GN   Name=yqkJ; OrderedLocusNames=BSU23550;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   MEDLINE=97124195; PubMed=8969508;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of
RT   the Bacillus subtilis genome containing the skin element and many
RT   sporulation genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 432-439.
RX   MEDLINE=93273731; PubMed=8501064;
RA   Smith K., Bayer M.E., Youngman P.;
RT   "Physical and functional characterization of the Bacillus subtilis
RT   spoIIM gene.";
RL   J. Bacteriol. 175:3607-3617(1993).
RN   [4]
RP   DISCUSSION OF FUNCTION.
RX   MEDLINE=20459096; PubMed=10903309; DOI=10.1074/jbc.M001112200;
RA   Wei Y., Guffanti A.A., Ito M., Krulwich T.A.;
RT   "Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that
RT   enhances growth on malate at low protonmotive force.";
RL   J. Biol. Chem. 275:30287-30292(2000).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
CC   -!- CAUTION: PubMed:10903309 has predicted this protein to be a
CC       malolactic enzyme which would convert malate to lactate.
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DR   EMBL; D84432; BAA12645.1; -; Genomic_DNA.
DR   EMBL; Z99116; CAB14287.1; -; Genomic_DNA.
DR   EMBL; L06664; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; C69967; C69967.
DR   RefSeq; NP_390236.1; -.
DR   GeneID; 938725; -.
DR   GenomeReviews; AL009126_GR; BSU23550.
DR   KEGG; bsu:BSU23550; -.
DR   NMPDR; fig|224308.1.peg.2360; -.
DR   SubtiList; BG11764; yqkJ.
DR   HOGENOM; P54572; -.
DR   BioCyc; BSUB224308:BSU2354-MON; -.
DR   GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002912; ACT_bd.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01842; ACT; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    439       Probable NAD-dependent malic enzyme 1.
FT                                /FTId=PRO_0000160208.
FT   NP_BIND     149    156       NAD (By similarity).
FT   ACT_SITE    112    112       Proton donor (By similarity).
FT   ACT_SITE    167    167       Proton acceptor (By similarity).
FT   METAL       209    209       Divalent metal cation (By similarity).
FT   METAL       210    210       Divalent metal cation (By similarity).
FT   BINDING     235    235       NAD (By similarity).
FT   BINDING     347    347       NAD (By similarity).
SQ   SEQUENCE   439 AA;  45890 MW;  2C6863E83CA86939 CRC64;
     MIAKHMIRTL MIETPSVPGN LGRVATAIGL LGGDIGEVET VKVGPNYTMR NITVQVENEE
     QLQEVIAAVQ ALGEGIRLHT VSDEVLSAHE GGKIQMKSKM PIRSLAELGR VYTPGVADVC
     RLIEKEPEKA SIYTTISNSV AIVTDGTAIL GLGNIGSVAG MPVMEGKAAL FDQLAGISGI
     PILLDTSDPE EIIKTVKHIS PGFSGILLED IGSPHCFEIE DRLKEELNIP VMHDDQHGTA
     VVTLAAAISA CRSAGVDLKE AKVGQIGLGA AGVAICRMFM AYGVNAVYGT DKSESAMNRL
     EQYGGQAVSS IEELMETCDI VIATTGVPGL IKPAFVRSGQ VILALSNPKP EIEPEAALQA
     GAAYAADGRS VNNVLGFPGI FRGALNAKST EINHDMLVAA AEAIAACTKQ GDVVPQPLDS
     KVHHAVAAAV EHAALTAVK
//