ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P54572


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name MAO1_BACSU
Primary accession number P54572
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 66)
Name and origin of the protein
Protein name Probable NAD-dependent malic enzyme 1
Synonyms NAD-ME 1
EC 1.1.1.38
Gene name
Name: yqkJ
OrderedLocusNames: BSU23550
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168 / JH642;
PubMed=8969508 [NCBI, ExPASy, EBI, Israel, Japan]
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.;
"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes.";
Microbiology 142:3103-3111(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 432-439.
PubMed=8501064 [NCBI, ExPASy, EBI, Israel, Japan]
Smith K., Bayer M.E., Youngman P.;
"Physical and functional characterization of the Bacillus subtilis spoIIM gene.";
J. Bacteriol. 175:3607-3617(1993).
[4]
DISCUSSION OF FUNCTION.
DOI=10.1074/jbc.M001112200; PubMed=10903309 [NCBI, ExPASy, EBI, Israel, Japan]
Wei Y., Guffanti A.A., Ito M., Krulwich T.A.;
"Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that enhances growth on malate at low protonmotive force.";
J. Biol. Chem. 275:30287-30292(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D84432; BAA12645.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99116; CAB14287.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L06664; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
PIR C69967; C69967.
RefSeq NP_390236.1; -.
3D structure databases
ModBase P54572.
Enzyme and pathway databases
BioCyc BSUB224308:BSU2354-MON; -.
Organism-specific databases
SubtiList BG11764; yqkJ. [Micado]
Ontologies
GO
GO:0016619; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR002912; ACT_bd.
IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF01842; ACT; 1.
PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS P54572.
Genome annotation databases
GeneID 938725; -.
GenomeReviews AL009126_GR; BSU23550.
KEGG bsu:BSU23550; -.
NMPDR fig|224308.1.peg.2360; -.
Phylogenomic databases
HOGENOM P54572; -.
Genome annotation databases
CMR P54572; BSU23550.
Other
ProtoNet P54572.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   439  439     Probable NAD-dependent malic enzyme 1. PRO_0000160208
NP_BIND   149   156  8     NAD (By similarity). 
ACT_SITE   112   112        Proton donor (By similarity). 
ACT_SITE   167   167        Proton acceptor (By similarity). 
METAL   209   209        Divalent metal cation (By similarity). 
METAL   210   210        Divalent metal cation (By similarity). 
BINDING   235   235        NAD (By similarity). 
BINDING   347   347        NAD (By similarity). 
Sequence information
Length: 439 AA [This is the length of the unprocessed precursor] Molecular weight: 45890 Da [This is the MW of the unprocessed precursor] CRC64: 2C6863E83CA86939 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MIAKHMIRTL MIETPSVPGN LGRVATAIGL LGGDIGEVET VKVGPNYTMR NITVQVENEE 

        70         80         90        100        110        120 
QLQEVIAAVQ ALGEGIRLHT VSDEVLSAHE GGKIQMKSKM PIRSLAELGR VYTPGVADVC 

       130        140        150        160        170        180 
RLIEKEPEKA SIYTTISNSV AIVTDGTAIL GLGNIGSVAG MPVMEGKAAL FDQLAGISGI 

       190        200        210        220        230        240 
PILLDTSDPE EIIKTVKHIS PGFSGILLED IGSPHCFEIE DRLKEELNIP VMHDDQHGTA 

       250        260        270        280        290        300 
VVTLAAAISA CRSAGVDLKE AKVGQIGLGA AGVAICRMFM AYGVNAVYGT DKSESAMNRL 

       310        320        330        340        350        360 
EQYGGQAVSS IEELMETCDI VIATTGVPGL IKPAFVRSGQ VILALSNPKP EIEPEAALQA 

       370        380        390        400        410        420 
GAAYAADGRS VNNVLGFPGI FRGALNAKST EINHDMLVAA AEAIAACTKQ GDVVPQPLDS 

       430 
KVHHAVAAAV EHAALTAVK 

P54572 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by kr flag YPRC Korea Mirror sites: Australia  Brazil  Canada  China  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!