ID   STMN1_MOUSE             Reviewed;         149 AA.
AC   P54227;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-DEC-2008, entry version 78.
DE   RecName: Full=Stathmin;
DE   AltName: Full=Phosphoprotein p19;
DE            Short=pp19;
DE   AltName: Full=Leukemia-associated phosphoprotein p18;
DE   AltName: Full=Oncoprotein 18;
DE            Short=Op18;
DE   AltName: Full=pp17;
DE   AltName: Full=Prosolin;
DE   AltName: Full=Metablastin;
DE   AltName: Full=Protein Pr22;
DE   AltName: Full=Leukemia-associated gene protein;
GN   Name=Stmn1; Synonyms=Lag, Lap18, Pr22;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=94117005; PubMed=8288240; DOI=10.1006/geno.1993.1477;
RA   Okazaki T., Yoshida B.N., Avraham K.B., Wang H., Wuenschell C.W.,
RA   Jenkins N.A., Copeland N.G., Anderson D.J., Mori N.;
RT   "Molecular diversity of the SCG10/stathmin gene family in the mouse.";
RL   Genomics 18:360-373(1993).
RN   [2]
RP   ERRATUM.
RA   Okazaki T., Yoshida B.N., Avraham K.B., Wang H., Wuenschell C.W.,
RA   Jenkins N.A., Copeland N.G., Anderson D.J., Mori N.;
RL   Genomics 21:298-298(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6;
RX   MEDLINE=97062620; PubMed=8906359;
RA   Hosoya H., Ishikawa K., Dohi N., Marunouchi T.;
RT   "Transcriptional and post-transcriptional regulation of pr22 (Op18)
RT   with proliferation control.";
RL   Cell Struct. Funct. 21:237-243(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=129; TISSUE=Mammary gland, and Retina;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PROTEIN SEQUENCE OF 15-27, AND MASS SPECTROMETRY.
RC   TISSUE=Brain, and Hippocampus;
RA   Lubec G., Klug S., Yang J.W., Zigmond M.;
RL   Submitted (JUL-2007) to UniProtKB.
RN   [6]
RP   PHOSPHORYLATION AT SER-16; SER-25; SER-38 AND SER-63.
RX   MEDLINE=93388570; PubMed=8376365;
RA   Beretta L., Dobransky T., Sobel A.;
RT   "Multiple phosphorylation of stathmin. Identification of four sites
RT   phosphorylated in intact cells and in vitro by cyclic AMP-dependent
RT   protein kinase and p34cdc2.";
RL   J. Biol. Chem. 268:20076-20084(1993).
RN   [7]
RP   INTERACTION WITH KIST.
RC   TISSUE=Embryo;
RX   MEDLINE=95241452; PubMed=7724523;
RA   Maucuer A., Camonis J.H., Sobel A.;
RT   "Stathmin interaction with a putative kinase and coiled-coil-forming
RT   protein domains.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3100-3104(1995).
RN   [8]
RP   FUNCTION IN CONTROL OF FEAR, AND TISSUE SPECIFICITY.
RX   PubMed=16286011; DOI=10.1016/j.cell.2005.08.038;
RA   Shumyatsky G.P., Malleret G., Shin R.-M., Takizawa S., Tully K.,
RA   Tsvetkov E., Zakharenko S.S., Joseph J., Vronskaya S., Yin D.,
RA   Schubart U.K., Kandel E.R., Bolshakov V.Y.;
RT   "Stathmin, a gene enriched in the amygdala, controls both learned and
RT   innate fear.";
RL   Cell 123:697-709(2005).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25; SER-38 AND SER-46,
RP   AND MASS SPECTROMETRY.
RC   TISSUE=Brain cortex;
RX   PubMed=17114649; DOI=10.1074/mcp.M600046-MCP200;
RA   Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,
RA   Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,
RA   Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
RT   "Qualitative and quantitative analyses of protein phosphorylation in
RT   naive and stimulated mouse synaptosomal preparations.";
RL   Mol. Cell. Proteomics 6:283-293(2007).
CC   -!- FUNCTION: Involved in the regulation of the microtubule (MT)
CC       filament system by destabilizing microtubules. Prevents assembly
CC       and promotes disassembly of microtubules. Phosphorylation at Ser-
CC       16 may be required for axon formation during neurogenesis (By
CC       similarity). Involved in the control of the learned and innate
CC       fear.
CC   -!- SUBUNIT: Binds to two alpha/beta-tubulin heterodimers. Interacts
CC       with KIST.
CC   -!- INTERACTION:
CC       P46414:Cdkn1b; NbExp=2; IntAct=EBI-1006438, EBI-1005742;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- TISSUE SPECIFICITY: Highly expressed in the lateral nucleus of the
CC       amygdala.
CC   -!- PTM: Many different phosphorylated forms are observed depending on
CC       specific combinations among the sites which can be phosphorylated.
CC       MAPK is responsible for the phosphorylation of stathmin in
CC       response to NGF (Probable). Phosphorylation at Ser-16 seems to be
CC       required for neuron polarization (By similarity).
CC   -!- MISCELLANEOUS: Mice lacking Stmn1 show deficits in spike-timing-
CC       dependent long-term potentiation, exhibit decreased memory in
CC       amygdala-dependent fear conditioning and fail to recognize danger
CC       in innately aversive environements.
CC   -!- SIMILARITY: Belongs to the stathmin family.
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DR   EMBL; L20256; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; L20257; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; L20258; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; X94915; CAA64401.1; -; mRNA.
DR   EMBL; BC010581; AAH10581.1; -; mRNA.
DR   EMBL; BC031831; AAH31831.1; -; mRNA.
DR   EMBL; BC054396; AAH54396.1; -; mRNA.
DR   PIR; B48917; B48917.
DR   RefSeq; NP_062615.1; -.
DR   UniGene; Mm.378957; -.
DR   IntAct; P54227; 1.
DR   PhosphoSite; P54227; -.
DR   REPRODUCTION-2DPAGE; P54227; -.
DR   Ensembl; ENSMUSG00000028832; Mus musculus.
DR   GeneID; 16765; -.
DR   KEGG; mmu:16765; -.
DR   MGI; MGI:96739; Stmn1.
DR   HOGENOM; P54227; -.
DR   HOVERGEN; P54227; -.
DR   NextBio; 290586; -.
DR   ArrayExpress; P54227; -.
DR   CleanEx; MM_STMN1; -.
DR   GermOnline; ENSMUSG00000028832; Mus musculus.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0007409; P:axonogenesis; IMP:MGI.
DR   GO; GO:0007242; P:intracellular signaling cascade; IEA:InterPro.
DR   GO; GO:0031115; P:negative regulation of microtubule polymeri...; IDA:MGI.
DR   GO; GO:0051272; P:positive regulation of cell motion; IGI:MGI.
DR   InterPro; IPR000956; Stathmin.
DR   PANTHER; PTHR10104; Stathmin; 1.
DR   Pfam; PF00836; Stathmin; 1.
DR   PIRSF; PIRSF002285; Stathmin; 1.
DR   PRINTS; PR00345; STATHMIN.
DR   PROSITE; PS00563; STATHMIN_1; 1.
DR   PROSITE; PS01041; STATHMIN_2; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Coiled coil; Cytoplasm; Developmental protein;
KW   Differentiation; Direct protein sequencing; Microtubule; Neurogenesis;
KW   Phosphoprotein.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    149       Stathmin.
FT                                /FTId=PRO_0000182390.
FT   COILED       41    140       Potential.
FT   MOD_RES       2      2       N-acetylalanine (By similarity).
FT   MOD_RES      16     16       Phosphoserine; by PKA.
FT   MOD_RES      25     25       Phosphoserine; by CDC2 and MAPK.
FT   MOD_RES      28     28       Phosphoserine (By similarity).
FT   MOD_RES      38     38       Phosphoserine; by CDC2.
FT   MOD_RES      46     46       Phosphoserine.
FT   MOD_RES      63     63       Phosphoserine; by PKA.
FT   MOD_RES     146    146       Phosphothreonine (By similarity).
SQ   SEQUENCE   149 AA;  17274 MW;  616526E0A6667BDA CRC64;
     MASSDIQVKE LEKRASGQAF ELILSPRSKE SVPDFPLSPP KKKDLSLEEI QKKLEAAEER
     RKSHEAEVLK QLAEKREHEK EVLQKAIEEN NNFSKMAEEK LTHKMEANKE NREAQMAAKL
     ERLREKDKHV EEVRKNKESK DPADETEAD
//