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UniProtKB/Swiss-Prot entry P54115


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ALDH6_YEAST
Primary accession number P54115
Secondary accession number Q02782
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    November 25, 2008 (Entry version 78)
Name and origin of the protein
Protein name Magnesium-activated aldehyde dehydrogenase, cytosolic
Synonyms EC 1.2.1.3
Mg(2+)-activated acetaldehyde dehydrogenase
Mg(2+)-ACDH
Gene name
Name: ALD6
Synonyms: ALDH1
OrderedLocusNames: YPL061W
ORFNames: LPE9
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
STRAIN=DBY939;
PubMed=9473035 [NCBI, ExPASy, EBI, Israel, Japan]
Wang X., Mann C.J., Bai Y., Ni L., Weiner H.;
"Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae.";
J. Bacteriol. 180:822-830(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169875 [NCBI, ExPASy, EBI, Israel, Japan]
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
Nature 387:103-105(1997).
[3]
PROTEIN SEQUENCE OF 76-79 AND 482-488.
STRAIN=ATCC 44827 / SKQ2N;
DOI=10.1074/jbc.272.9.5544; PubMed=9038161 [NCBI, ExPASy, EBI, Israel, Japan]
Norbeck J., Blomberg A.;
"Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway.";
J. Biol. Chem. 272:5544-5554(1997).
[4]
PROTEIN SEQUENCE OF 2-16, AND CHARACTERIZATION.
DOI=10.1002/(SICI)1097-0061(199711)13:14<1319::AID-YEA183>3.0.CO;2-T; PubMed=9392076 [NCBI, ExPASy, EBI, Israel, Japan]
Meaden P.G., Dickinson F.M., Mifsud A., Tessier W., Westwater J., Bussey H., Midgley M.;
"The ALD6 gene of Saccharomyces cerevisiae encodes a cytosolic, Mg(2+)-activated acetaldehyde dehydrogenase.";
Yeast 13:1319-1327(1997).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-3, AND MASS SPECTROMETRY.
DOI=10.1038/nbt849; PubMed=12872131 [NCBI, ExPASy, EBI, Israel, Japan]
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.;
"A proteomics approach to understanding protein ubiquitination.";
Nat. Biotechnol. 21:921-926(2003).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-491, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0607084104; PubMed=17287358 [NCBI, ExPASy, EBI, Israel, Japan]
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250; THR-251 AND SER-262, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U56604; AAB01219.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U39205; AAB68304.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S60929; S60929.
RefSeq NP_015264.1; -.
3D structure databases
HSSP Q28399; 1O9J. [HSSP ENTRY / PDB]
ModBase P54115.
Protein-protein interaction databases
IntAct P54115; -.
Enzyme and pathway databases
BioCyc MetaCyc:MON-13664; -.
Organism-specific databases
CYGD YPL061w; -.
SGD S000005982; ALD6.
Yeast-GFP YPL061W.
Gene expression databases
GermOnline YPL061W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from SGD).
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0004029; Molecular function: aldehyde dehydrogenase (NAD) activity (inferred from electronic annotation from EC).
GO:0004030; Molecular function: aldehyde dehydrogenase [NAD(P)+] activity (inferred from direct assay from SGD).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0019413; Biological process: acetate biosynthetic process (inferred from mutant phenotype from SGD).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
PANTHER PTHR11699; Aldehyde_dehyd; 1.
Pfam PF00171; Aldedh; 1.
Pfam graphical view of domain structure.
PROSITE PS00070; ALDEHYDE_DEHYDR_CYS; FALSE_NEG.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.
ProtoNet P54115.
Proteomic databases
PeptideAtlas P54115; -.
Genome annotation databases
Ensembl YPL061W; Saccharomyces cerevisiae. [Contig view]
GeneID 856044; -.
KEGG sce:YPL061W; -.
NMPDR fig|4932.3.peg.6397; -.
Phylogenomic databases
HOGENOM P54115; -.
Other
LinkHub P54115; -.
NextBio 980989; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Direct protein sequencing; Magnesium; NAD; Oxidoreductase; Phosphoprotein; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   500  499     Magnesium-activated aldehyde dehydrogenase, cytosolic. PRO_0000056441
NP_BIND   249   254  6     NAD (By similarity). 
ACT_SITE   272   272        Proton acceptor (By similarity). 
ACT_SITE   306   306        Nucleophile (By similarity). 
SITE   173   173  1     Transition state stabilizer (By similarity). 
MOD_RES   250   250        Phosphoserine. 
MOD_RES   251   251        Phosphothreonine. 
MOD_RES   262   262        Phosphoserine. 
MOD_RES   491   491        Phosphothreonine. 
CROSSLNK   3     3        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin). 
CONFLICT   121   121        L -> FK (in Ref. 1; AAB01219). 
Sequence information
Length: 500 AA [This is the length of the unprocessed precursor] Molecular weight: 54414 Da [This is the MW of the unprocessed precursor] CRC64: 542AA956EFA0E676 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTKLHFDTAE PVKITLPNGL TYEQPTGLFI NNKFMKAQDG KTYPVEDPST ENTVCEVSSA 

        70         80         90        100        110        120 
TTEDVEYAIE CADRAFHDTE WATQDPRERG RLLSKLADEL ESQIDLVSSI EALDNGKTLA 

       130        140        150        160        170        180 
LARGDVTIAI NCLRDAAAYA DKVNGRTINT GDGYMNFTTL EPIGVCGQII PWNFPIMMLA 

       190        200        210        220        230        240 
WKIAPALAMG NVCILKPAAV TPLNALYFAS LCKKVGIPAG VVNIVPGPGR TVGAALTNDP 

       250        260        270        280        290        300 
RIRKLAFTGS TEVGKSVAVD SSESNLKKIT LELGGKSAHL VFDDANIKKT LPNLVNGIFK 

       310        320        330        340        350        360 
NAGQICSSGS RIYVQEGIYD ELLAAFKAYL ETEIKVGNPF DKANFQGAIT NRQQFDTIMN 

       370        380        390        400        410        420 
YIDIGKKEGA KILTGGEKVG DKGYFIRPTV FYDVNEDMRI VKEEIFGPVV TVAKFKTLEE 

       430        440        450        460        470        480 
GVEMANSSEF GLGSGIETES LSTGLKVAKM LKAGTVWINT YNDFDSRVPF GGVKQSGYGR 

       490        500 
EMGEEVYHAY TEVKAVRIKL 

P54115 in FASTA format

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View entry in raw text format (no links)
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