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UniProtKB/Swiss-Prot entry P53982

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IDHH_YEAST
Primary accession number P53982
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 63)
Name and origin of the protein
Protein name Isocitrate dehydrogenase [NADP]
Synonyms IDH
EC 1.1.1.42
Oxalosuccinate decarboxylase
NADP(+)-specific ICDH
IDP
Gene name
Name: IDP3
OrderedLocusNames: YNL009W
ORFNames: N2870
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169873 [NCBI, ExPASy, EBI, Israel, Japan]
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.";
Nature 387:93-98(1997).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z71285; CAA95869.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY693154; AAT93173.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S62921; S62921.
RefSeq NP_014389.1; -.
3D structure databases
HSSP P33198; 1LWD. [HSSP ENTRY / PDB]
SMR P53982; 3-407.
ModBase P53982.
Protein-protein interaction databases
DIP DIP:4693N; -.
IntAct P53982; -.
Organism-specific databases
CYGD YNL009w; -.
SGD S000004954; IDP3.
Yeast-GFP YNL009W.
Gene expression databases
GermOnline YNL009W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0005777; Cellular component: peroxisome (inferred from direct assay from SGD).
GO:0004450; Molecular function: isocitrate dehydrogenase (NADP+) activity (traceable author statement from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006635; Biological process: fatty acid beta-oxidation (inferred from mutant phenotype from SGD).
GO:0006102; Biological process: isocitrate metabolic process (traceable author statement from SGD).
GO:0006740; Biological process: NADPH regeneration (traceable author statement from SGD).
QuickGo view.
Family and domain databases
InterPro IPR004790; IsoCit_DHase_NADP-dep_euk.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11822; IDH_NADP_euk; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000108; IDH_NADP; 1.
TIGRFAMs TIGR00127; nadp_idh_euk; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS P53982.
Proteomic databases
PeptideAtlas P53982; -.
Genome annotation databases
Ensembl YNL009W; Saccharomyces cerevisiae. [Contig view]
GeneID 855723; -.
GenomeReviews Y13139_GR; YNL009W.
KEGG sce:YNL009W; -.
NMPDR fig|4932.3.peg.5467; -.
Phylogenomic databases
HOGENOM P53982; -.
Other
LinkHub P53982; -.
ProtoNet P53982.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   420  420     Isocitrate dehydrogenase [NADP]. PRO_0000083588
NP_BIND   75    77  3     NADP (By similarity). 
NP_BIND   310   315  6     NADP (By similarity). 
REGION   94   100  7     Substrate binding (By similarity). 
METAL   252   252        Magnesium or manganese (By similarity). 
METAL   275   275        Magnesium or manganese (By similarity). 
BINDING   77    77        Substrate (By similarity). 
BINDING   82    82        NADP (By similarity). 
BINDING   109   109        Substrate (By similarity). 
BINDING   132   132        Substrate (By similarity). 
BINDING   260   260        NADP (By similarity). 
BINDING   328   328        NADP; via amide nitrogen and carbonyl oxygen (By similarity). 
SITE   139   139  1     Critical for catalysis (By similarity). 
SITE   212   212  1     Critical for catalysis (By similarity). 
Sequence information
Length: 420 AA [This is the length of the unprocessed precursor] Molecular weight: 47856 Da [This is the MW of the unprocessed precursor] CRC64: 4094007E13FCD506 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSKIKVVHPI VEMDGDEQTR VIWKLIKEKL ILPYLDVDLK YYDLSIQERD RTNDQVTKDS 

        70         80         90        100        110        120 
SYATLKYGVA VKCATITPDE ARMKEFNLKE MWKSPNGTIR NILGGTVFRE PIIIPKIPRL 

       130        140        150        160        170        180 
VPHWEKPIII GRHAFGDQYR ATDIKIKKAG KLRLQFSSDD GKENIDLKVY EFPKSGGIAM 

       190        200        210        220        230        240 
AMFNTNDSIK GFAKASFELA LKRKLPLFFT TKNTILKNYD NQFKQIFDNL FDKEYKEKFQ 

       250        260        270        280        290        300 
ALKITYEHRL IDDMVAQMLK SKGGFIIAMK NYDGDVQSDI VAQGFGSLGL MTSILITPDG 

       310        320        330        340        350        360 
KTFESEAAHG TVTRHFRKHQ RGEETSTNSI ASIFAWTRAI IQRGKLDNTD DVIKFGNLLE 

       370        380        390        400        410        420 
KATLDTVQVG GKMTKDLALM LGKTNRSSYV TTEEFIDEVA KRLQNMMLSS NEDKKGMCKL
P53982 in FASTA format

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