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UniProtKB/Swiss-Prot entry P53184


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PNC1_YEAST
Primary accession number P53184
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 70)
Name and origin of the protein
Protein name Nicotinamidase
Synonyms EC 3.5.1.19
Nicotine deamidase
NAMase
Gene name
Name: PNC1
OrderedLocusNames: YGL037C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169869 [NCBI, ExPASy, EBI, Israel, Japan]
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
Nature 387:81-84(1997).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[3]
PROTEIN SEQUENCE OF 1-11, AND FUNCTION.
DOI=10.1002/yea.810.abs; PubMed=11816029 [NCBI, ExPASy, EBI, Israel, Japan]
Ghislain M., Talla E., Francois J.M.;
"Identification and functional analysis of the Saccharomyces cerevisiae nicotinamidase gene, PNC1.";
Yeast 19:215-224(2002).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z72559; CAA96739.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY558481; AAS56807.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S64039; S64039.
RefSeq NP_011478.1; -.
3D structure databases
PDB
2H0R; X-ray; 2.90 A; A/B/C/D/E/F/G=1-216.[ExPASy / RCSB / EBI]
PDBsum 2H0R; -.
ModBase P53184.
Protein-protein interaction databases
DIP DIP:2070N; -.
IntAct P53184; 10.
Organism-specific databases
CYGD YGL037c; -.
SGD S000003005; PNC1.
Yeast-GFP YGL037C.
Gene expression databases
ArrayExpress P53184; -.
GermOnline YGL037C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from direct assay from SGD).
GO:0005777; Cellular component: peroxisome (inferred from direct assay from SGD).
GO:0008936; Molecular function: nicotinamidase activity (inferred from electronic annotation from EC).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0000183; Biological process: chromatin silencing at rDNA (inferred from mutant phenotype from SGD).
GO:0006348; Biological process: chromatin silencing at telomere (inferred from mutant phenotype from SGD).
GO:0019363; Biological process: pyridine nucleotide biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0001302; Biological process: replicative cell aging (inferred from expression pattern from SGD).
QuickGo view.
Family and domain databases
InterPro IPR000868; Isochorismatase_hydro.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.850; Isochorismatase_hydro; 1.
Pfam PF00857; Isochorismatase; 1.
Pfam graphical view of domain structure.
Proteomic databases
PeptideAtlas P53184; -.
Proteomics databases
PRIDE P53184; -.
Genome annotation databases
Ensembl YGL037C; Saccharomyces cerevisiae. [Contig view]
GeneID 852846; -.
GenomeReviews Y13135_GR; YGL037C.
KEGG sce:YGL037C; -.
NMPDR fig|4932.3.peg.2585; -.
Phylogenomic databases
HOGENOM P53184; -.
Other
LinkHub P53184; -.
NextBio 972431; -.
ProtoNet P53184.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Direct protein sequencing; Hydrolase; Phosphoprotein; Pyridine nucleotide biosynthesis.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   216  216     Nicotinamidase. PRO_0000206558
MOD_RES   174   174        Phosphoserine. 
STRAND   2     7  6      
HELIX   11    14  4      
HELIX   26    28  3      
HELIX   29    37  9      
HELIX   39    41  3      
STRAND   44    51  8      
HELIX   61    63  3      
STRAND   71    76  6      
STRAND   85    91  7      
HELIX   100   102  3      
HELIX   106   114  9      
STRAND   118   123  6      
STRAND   132   135  4      
HELIX   145   151  7      
STRAND   155   162  8      
TURN   164   166  3      
HELIX   167   177  11      
STRAND   181   189  9      
HELIX   197   208  12      
STRAND   212   214  3      
Sequence information
Length: 216 AA [This is the length of the unprocessed precursor] Molecular weight: 24993 Da [This is the MW of the unprocessed precursor] CRC64: C40B23C4228B6E3A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKTLIVVDMQ NDFISPLGSL TVPKGEELIN PISDLMQDAD RDWHRIVVTR DWHPSRHISF 

        70         80         90        100        110        120 
AKNHKDKEPY STYTYHSPRP GDDSTQEGIL WPVHCVKNTW GSQLVDQIMD QVVTKHIKIV 

       130        140        150        160        170        180 
DKGFLTDREY YSAFHDIWNF HKTDMNKYLE KHHTDEVYIV GVALEYCVKA TAISAAELGY 

       190        200        210 
KTTVLLDYTR PISDDPEVIN KVKEELKAHN INVVDK 

P53184 in FASTA format

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