ID   LDHD_ECOLI              Reviewed;         329 AA.
AC   P52643; P78152;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-DEC-2008, entry version 69.
DE   RecName: Full=D-lactate dehydrogenase;
DE            Short=D-LDH;
DE            EC=1.1.1.28;
DE   AltName: Full=Fermentative lactate dehydrogenase;
GN   Name=ldhA; Synonyms=hslI, htpH; OrderedLocusNames=b1380, JW1375;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   MEDLINE=97177787; PubMed=9025293;
RA   Bunch P.K., Mat-Jan F., Lee N., Clark D.P.;
RT   "The ldhA gene encoding the fermentative lactate dehydrogenase of
RT   Escherichia coli.";
RL   Microbiology 143:187-195(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   MEDLINE=97251357; PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
RA   Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
RA   Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
RA   Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
RA   Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   CHARACTERIZATION AS AN HEAT-SHOCK GENE.
RX   MEDLINE=93352431; PubMed=8349564;
RA   Chuang S.E., Blattner F.R.;
RT   "Characterization of twenty-six new heat shock genes of Escherichia
RT   coli.";
RL   J. Bacteriol. 175:5242-5252(1993).
CC   -!- FUNCTION: Fermentative lactate dehydrogenase.
CC   -!- CATALYTIC ACTIVITY: (R)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family.
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DR   EMBL; U36928; AAB51772.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74462.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA14990.1; -; Genomic_DNA.
DR   PIR; G64888; G64888.
DR   RefSeq; AP_002007.1; -.
DR   RefSeq; NP_415898.1; -.
DR   HSSP; P30901; 2DLD.
DR   ECO2DBASE; D033.4; 6TH EDITION.
DR   GeneID; 946315; -.
DR   GenomeReviews; AP009048_GR; JW1375.
DR   GenomeReviews; U00096_GR; b1380.
DR   KEGG; ecj:JW1375; -.
DR   KEGG; eco:b1380; -.
DR   EchoBASE; EB2978; -.
DR   EcoGene; EG13186; ldhA.
DR   HOGENOM; P52643; -.
DR   BioCyc; EcoCyc:DLACTDEHYDROGNAD-MON; -.
DR   BioCyc; MetaCyc:DLACTDEHYDROGNAD-MON; -.
DR   GO; GO:0008720; F:D-lactate dehydrogenase activity; IEA:EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006950; P:response to stress; IEA:UniProtKB-KW.
DR   InterPro; IPR006139; D-isomer_2_OHA_DHase.
DR   InterPro; IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00670; D_2_HYDROXYACID_DH_2; 1.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; NAD; Oxidoreductase; Stress response.
FT   CHAIN         1    329       D-lactate dehydrogenase.
FT                                /FTId=PRO_0000075952.
FT   ACT_SITE    234    234       By similarity.
FT   ACT_SITE    263    263       By similarity.
FT   ACT_SITE    295    295       Proton donor (By similarity).
SQ   SEQUENCE   329 AA;  36535 MW;  EF85419988438D6D CRC64;
     MKLAVYSTKQ YDKKYLQQVN ESFGFELEFF DFLLTEKTAK TANGCEAVCI FVNDDGSRPV
     LEELKKHGVK YIALRCAGFN NVDLDAAKEL GLKVVRVPAY DPEAVAEHAI GMMMTLNRRI
     HRAYQRTRDA NFSLEGLTGF TMYGKTAGVI GTGKIGVAML RILKGFGMRL LAFDPYPSAA
     ALELGVEYVD LPTLFSESDV ISLHCPLTPE NYHLLNEAAF EQMKNGVMIV NTSRGALIDS
     QAAIEALKNQ KIGSLGMDVY ENERDLFFED KSNDVIQDDV FRRLSACHNV LFTGHQAFLT
     AEALTSISQT TLQNLSNLEK GETCPNELV
//