[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=Hereford; Bovine sequencing consortium; Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 284-409. TISSUE=Cornea; PubMed=8493893 [NCBI, ExPASy, EBI, Israel, Japan] Algar E.M., Cheung B., Hayes J., Holmes R.S., Beacham I.R.; "Bovine corneal aldehyde dehydrogenases: evidence for multiple gene products (ALDH3 and ALDHX)."; Adv. Exp. Med. Biol. 328:153-157(1993).

Comments

FUNCTION: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. In the cornea, this enzyme may help in the absorption of the damaging UV-B, as well as in the detoxification of the UV-induced peroxidic aldehydes.
CATALYTIC ACTIVITY: An aldehyde + NAD⁺ + H₂O = an acid + NADH.
PATHWAY: Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2 .
SUBUNIT: Homotetramer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
SIMILARITY: Belongs to the aldehyde dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AAFC03050684; -; NOT_ANNOTATED_CDS; Genomic_DNA.	[EMBL / GenBank / DDBJ]
S61045; AAB26659.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I46935; I46935.

UniGene

Bt.13145

3D structure databases

HSSP

P05091; 1O02. [HSSP ENTRY / PDB]

ModBase

P52476.

Ontologies

GO:0005759;	Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
GO:0004029;	Molecular function: aldehyde dehydrogenase (NAD) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11699; Aldehyde_dehyd; 1.

Pfam

PF00171; Aldedh; 1.
Pfam graphical view of domain structure.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; FALSE_NEG.
PS00687; ALDEHYDE_DEHYDR_GLU; FALSE_NEG.

Genome annotation databases

Ensembl

ENSBTAG00000020385; Bos taurus. [Contig view]

Phylogenomic databases

HOVERGEN

P52476; -.

Other

ProtoNet

P52476.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; NAD; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`<1 11`	`>11`	`Mitochondrion (Potential).`
`CHAIN`	`12 511`	`500`	`Aldehyde dehydrogenase X, mitochondrial.`	`PRO_0000056477`
`NP_BIND`	`256 261`	`6`	`NAD (By similarity).`
`ACT_SITE`	`279 279`		`Proton acceptor (By similarity).`
`ACT_SITE`	`313 313`		`Nucleophile (By similarity).`
`SITE`	`180 180`	`1`	`Transition state stabilizer (By similarity).`
`CONFLICT`	`308 308`		`N -> S (in Ref. 2; AAB26659).`
`NON_TER`	`1 1`

Sequence information

Length: 511 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 56770 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: D032C6E84D9799B3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
PRLFALHHSA TQYFSAAALP SPIPNPDIPD NQLFISNKWH DAVSKKTFPT VSPATGEVIG 

        70         80         90        100        110        120 
HVAEGDWADV DLAAKAARAA FRLGSPWRWM DALKRGWLLN HLADLVERDC VYLASLESLD 

       130        140        150        160        170        180 
NGKPFQESYV LDLDEVIKVY RYFAGWADKW HGKTIPMDGE HFCFTRHEPV GVCCQIIPWN 

       190        200        210        220        230        240 
FPLVMQSWKL ALALAMGNTV VTKVAEQTPF SALYLASLIK EVGLPPGLVN IVTGYGPTAG 

       250        260        270        280        290        300 
AAIAHHMDIG KVAFTGSTKV GHLIQKAAGN SSLKRVTLEL GGKSLSIVLA DADMDHAVEQ 

       310        320        330        340        350        360 
RQEALFFNMG QCCCPGSWTF IEESIYDEFL ERTVEKAKQR RVGNPFDLDT QQGPQVDRER 

       370        380        390        400        410        420 
FERILGYIQL GQKEGAKLLC GGEHFRQQCF FIKPTVFGGV QDDMRIAREE IFGPVQPLFK 

       430        440        450        460        470        480 
FKKIEEVIER ADNTRYGLAA AVFTQDLDKA MYFTQALQTG TVWVNTYNVV TCHTPLGGFK 

       490        500        510 
EPGNGRELGE DGLKAYTEVK TVTIKVPQKN S

P52476 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools