ID   GPDA_CUPLA              Reviewed;         372 AA.
AC   P52425;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-NOV-2008, entry version 46.
DE   RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD+];
DE            EC=1.1.1.8;
GN   Name=GPDH;
OS   Cuphea lanceolata.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; Myrtales; Lythraceae; Cuphea.
OX   NCBI_TaxID=3930;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Hausmann L., Schell J., Toepfer R.;
RT   "Cloning of a cDNA coding for a glycerol-3-phosphate dehydrogenase
RT   from Cuphea lanceolata.";
RL   (In) Kader J.-C., Mazliak P. (eds.);
RL   Plant lipid metabolism, pp.534-536, Kluwer Academic Publishers,
RL   Dordrecht (1995).
CC   -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(+) = glycerone
CC       phosphate + NADH.
CC   -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; X79677; CAA56125.1; -; mRNA.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase (NAD+) a...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013328; DHase_multihelical.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   InterPro; IPR017751; NAD-dep_Gly3P_DH_euk.
DR   InterPro; IPR006168; NAD-dep_Gly3P_DHase.
DR   InterPro; IPR011128; NAD-dep_Gly3P_DHase_N.
DR   InterPro; IPR006109; NAD_Gly3P_DHase_C.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR11728; NAD_Gly3P_DH; 1.
DR   Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR   PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR   PRINTS; PR00077; GPDHDRGNASE.
DR   ProDom; PD001278; NAD_Gly3P_C; 1.
DR   PROSITE; PS00957; NAD_G3PDH; 1.
PE   2: Evidence at transcript level;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    372       Glycerol-3-phosphate dehydrogenase
FT                                [NAD+].
FT                                /FTId=PRO_0000138071.
FT   NP_BIND      29     34       NAD (By similarity).
FT   REGION      289    290       Substrate binding (By similarity).
FT   ACT_SITE    225    225       Proton acceptor (By similarity).
FT   BINDING     117    117       NAD (By similarity).
FT   BINDING     140    140       NAD; via amide nitrogen (By similarity).
FT   BINDING     140    140       Substrate (By similarity).
FT   BINDING     173    173       NAD; via amide nitrogen (By similarity).
FT   BINDING     289    289       NAD (By similarity).
FT   BINDING     320    320       NAD (By similarity).
SQ   SEQUENCE   372 AA;  40811 MW;  1BF91793CDC23AA9 CRC64;
     MAPSELNCTH QNQHSSGYDG PRSRVTVVGS GNWGSVAAKL IATNTLKLPS FHDEVRMWVF
     EETLPSGEKL TDVINQTNEN VKYLPGIKLG RNVVADPDLE NAVKDANMLV FVTPHQFMEG
     ICKRLEGKIQ EGAQALSLIK GMEVKMEGPC MISSLISDLL GINCCVLMGA NIANEIAVEK
     FSEATVGFRE NRDIAEKWVQ LFSTPYFMVS AVEDVEGVEL CGTLKNIVAI AAGFVDGLEM
     GNNTKAAIMR IGLREMKAFS KLLFPSVKDT TFFESCGVAD LITTCLGGRN RKVAEAFAKN
     GGKRSFDDLE AEMLRGQKLQ GVSTAKEVYE VLGHRGWLEL FPLFSTVHEI STGRLPPSAI
     VEYSEQKTIF SW
//