ID   SSDH_RAT                Reviewed;         523 AA.
AC   P51650;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 2.
DT   22-JUL-2008, entry version 67.
DE   RecName: Full=Succinate-semialdehyde dehydrogenase, mitochondrial;
DE            EC=1.2.1.24;
DE   AltName: Full=NAD(+)-dependent succinic semialdehyde dehydrogenase;
DE   AltName: Full=Aldehyde dehydrogenase family 5 member A1;
DE   Flags: Precursor;
GN   Name=Aldh5a1; Synonyms=Ssadh;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T.,
RA   Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A.,
RA   Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M.,
RA   Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K.,
RA   Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S.,
RA   Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J.,
RA   Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y.,
RA   Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A.,
RA   Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J.,
RA   D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R.,
RA   Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A.,
RA   Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E.,
RA   Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E.,
RA   Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D.,
RA   Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M.,
RA   Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O.,
RA   Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O.,
RA   Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H.,
RA   Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S.,
RA   Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J.,
RA   Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E.,
RA   Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F.,
RA   Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K.,
RA   Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S.,
RA   Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M.,
RA   Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M.,
RA   Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A.,
RA   Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S.,
RA   Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G.,
RA   Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H.,
RA   Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R.,
RA   Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M.,
RA   Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H.,
RA   Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S.,
RA   Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into
RT   mammalian evolution.";
RL   Nature 428:493-521(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), AND PROTEIN
RP   SEQUENCE OF 36-84; 92-101; 129-153; 162-172; 303-311; 419-425 AND
RP   503-526.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RX   MEDLINE=95113870; PubMed=7814412; DOI=10.1074/jbc.270.1.461;
RA   Chambliss K.L., Caudle D.L., Hinson D.D., Moomaw C.R., Slaughter C.A.,
RA   Jakobs C., Gibson K.M.;
RT   "Molecular cloning of the mature NAD(+)-dependent succinic
RT   semialdehyde dehydrogenase from rat and human. cDNA isolation,
RT   evolutionary homology, and tissue expression.";
RL   J. Biol. Chem. 270:461-467(1995).
RN   [3]
RP   PROTEIN SEQUENCE OF 36-48; 161-172 AND 401-418, AND MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Spinal cord;
RA   Lubec G., Afjehi-Sadat L.;
RL   Submitted (NOV-2006) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY: Succinate semialdehyde + NAD(+) + H(2)O =
CC       succinate + NADH.
CC   -!- PATHWAY: Amino-acid degradation; 4-aminobutanoate degradation.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Long;
CC         IsoId=P51650-1; Sequence=Displayed;
CC       Name=Short;
CC         IsoId=P51650-2; Sequence=VSP_001284;
CC         Note=No experimental confirmation available;
CC   -!- TISSUE SPECIFICITY: Brain, pancreas, heart, liver, skeletal
CC       muscle, kidney. Lower in spleen, lung, kidney and testis.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; AABR03105019; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; L34821; AAA67058.1; -; mRNA.
DR   PIR; I61704; I61704.
DR   UniGene; Rn.10070; -.
DR   HSSP; P51977; 1BXS.
DR   Ensembl; ENSRNOG00000023538; Rattus norvegicus.
DR   RGD; 621422; Aldh5a1.
DR   HOVERGEN; P51650; -.
DR   ArrayExpress; P51650; -.
DR   GermOnline; ENSRNOG00000023538; Rattus norvegicus.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0004777; F:succinate-semialdehyde dehydrogenase activity; IDA:UniProtKB.
DR   GO; GO:0007417; P:central nervous system development; TAS:UniProtKB.
DR   GO; GO:0009450; P:gamma-aminobutyric acid catabolic process; TAS:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IDA:UniProtKB.
DR   InterPro; IPR016160; Ald_DHase_CS.
DR   InterPro; IPR016162; Ald_DHase_N.
DR   InterPro; IPR015590; Aldehyde_DHase.
DR   InterPro; IPR010102; Succ_semiAld_DHase.
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   TIGRFAMs; TIGR01780; SSADH; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Direct protein sequencing; Mitochondrion; NAD;
KW   Oxidoreductase; Phosphoprotein; Transit peptide.
FT   TRANSIT       1     35       Mitochondrion.
FT   CHAIN        36    523       Succinate-semialdehyde dehydrogenase,
FT                                mitochondrial.
FT                                /FTId=PRO_0000056567.
FT   NP_BIND     272    277       NADP (By similarity).
FT   ACT_SITE    294    294       Proton acceptor (By similarity).
FT   ACT_SITE    328    328       Nucleophile (By similarity).
FT   SITE        193    193       Transition state stabilizer (By
FT                                similarity).
FT   MOD_RES      67     67       Phosphotyrosine (By similarity).
FT   VAR_SEQ     107    134       Missing (in isoform Short).
FT                                /FTId=VSP_001284.
SQ   SEQUENCE   523 AA;  56131 MW;  4CA521139C9FA98F CRC64;
     MATCFLLRNF CAARPALRPP GRLLREPAGA QRRSYVGGPA DLHADLLRGD SFVGGRWLPT
     PATFPVYDPA SGAKLGTVAD CGVPEARAAV RAAYDAFSSW KEISVKERSS LLRKWYDLMI
     QNKDELAKII TAESGKPLKE AQGEILYSAF FLEWFSEEAR RVYGDIIYTS AKDKRGLVLK
     QPVGVASIIT PWNFPSAMIT RKVGAALAAG CTVVVKPAED TPYSALALAQ LANQAGIPPG
     VYNVIPCSRT KAKEVGEVLC TDPLVSKISF TGSTATGKIL LHHAANSVKR VSMELGGLAP
     FIVFDSANVD QAVAGAMASK FRNAGQTCVC SNRFLVQRGI HDSFVTKFAE AMKKSLRVGN
     GFEEGTTQGP LINEKAVEKV EKHVNDAVAK GATVVTGGKR HQSGGNFFEP TLLSNVTRDM
     LCITEETFGP VAPVIKFDKE EEAVAIANAA DVGLAGYFYS QDPAQIWRVA EQLEVGMVGV
     NEGLISSVEC PFGGVKQSGL GREGSKYGID EYLEVKYVCY GGL
//