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UniProtKB/Swiss-Prot entry P51650


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SSDH_RAT
Primary accession number P51650
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on April 8, 2008 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 67)
Name and origin of the protein
Protein name Succinate-semialdehyde dehydrogenase, mitochondrial [Precursor]
Synonyms EC 1.2.1.24
NAD(+)-dependent succinic semialdehyde dehydrogenase
Aldehyde dehydrogenase family 5 member A1
Gene name
Name: Aldh5a1
Synonyms: Ssadh
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Brown Norway;
DOI=10.1038/nature02426; PubMed=15057822 [NCBI, ExPASy, EBI, Israel, Japan]
Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.;
"Genome sequence of the Brown Norway rat yields insights into mammalian evolution.";
Nature 428:493-521(2004).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), AND PROTEIN SEQUENCE OF 36-84; 92-101; 129-153; 162-172; 303-311; 419-425 AND 503-526.
STRAIN=Sprague-Dawley;
TISSUE=Brain;
DOI=10.1074/jbc.270.1.461; PubMed=7814412 [NCBI, ExPASy, EBI, Israel, Japan]
Chambliss K.L., Caudle D.L., Hinson D.D., Moomaw C.R., Slaughter C.A., Jakobs C., Gibson K.M.;
"Molecular cloning of the mature NAD(+)-dependent succinic semialdehyde dehydrogenase from rat and human. cDNA isolation, evolutionary homology, and tissue expression.";
J. Biol. Chem. 270:461-467(1995).
[3]
PROTEIN SEQUENCE OF 36-48; 161-172 AND 401-418, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Spinal cord;
Lubec G., Afjehi-Sadat L.;
Submitted (NOV-2006) to UniProtKB.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AABR03105019; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
L34821; AAA67058.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR I61704; I61704.
UniGene Rn.10070
3D structure databases
HSSP P51977; 1BXS. [HSSP ENTRY / PDB]
ModBase P51650.
Organism-specific databases
RGD 621422; Aldh5a1.
Gene expression databases
ArrayExpress P51650; -.
GermOnline ENSRNOG00000023538; Rattus norvegicus.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from sequence or structural similarity from UniProtKB).
GO:0004777; Molecular function: succinate-semialdehyde dehydrogenase activity (inferred from direct assay from UniProtKB).
GO:0007417; Biological process: central nervous system development (traceable author statement from UniProtKB).
GO:0009450; Biological process: gamma-aminobutyric acid catabolic process (traceable author statement from UniProtKB).
GO:0055114; Biological process: oxidation reduction (inferred from direct assay from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
IPR010102; Succ_semiAld_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
PANTHER PTHR11699; Aldehyde_dehyd; 1.
Pfam PF00171; Aldedh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01780; SSADH; 1.
PROSITE PS00070; ALDEHYDE_DEHYDR_CYS; 1.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.
BLOCKS P51650.
Genome annotation databases
Ensembl ENSRNOG00000023538; Rattus norvegicus. [Contig view]
Phylogenomic databases
HOVERGEN P51650; -.
Other
ProtoNet P51650.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    35  35     Mitochondrion. 
CHAIN   36   523  488     Succinate-semialdehyde dehydrogenase, mitochondrial. PRO_0000056567
NP_BIND   272   277  6     NADP (By similarity). 
ACT_SITE   294   294        Proton acceptor (By similarity). 
ACT_SITE   328   328        Nucleophile (By similarity). 
SITE   193   193  1     Transition state stabilizer (By similarity). 
MOD_RES   67    67        Phosphotyrosine (By similarity). 
VAR_SEQ   107   134        Missing (in isoform Short). VSP_001284
Sequence information
Length: 523 AA [This is the length of the unprocessed precursor] Molecular weight: 56131 Da [This is the MW of the unprocessed precursor] CRC64: 4CA521139C9FA98F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MATCFLLRNF CAARPALRPP GRLLREPAGA QRRSYVGGPA DLHADLLRGD SFVGGRWLPT 

        70         80         90        100        110        120 
PATFPVYDPA SGAKLGTVAD CGVPEARAAV RAAYDAFSSW KEISVKERSS LLRKWYDLMI 

       130        140        150        160        170        180 
QNKDELAKII TAESGKPLKE AQGEILYSAF FLEWFSEEAR RVYGDIIYTS AKDKRGLVLK 

       190        200        210        220        230        240 
QPVGVASIIT PWNFPSAMIT RKVGAALAAG CTVVVKPAED TPYSALALAQ LANQAGIPPG 

       250        260        270        280        290        300 
VYNVIPCSRT KAKEVGEVLC TDPLVSKISF TGSTATGKIL LHHAANSVKR VSMELGGLAP 

       310        320        330        340        350        360 
FIVFDSANVD QAVAGAMASK FRNAGQTCVC SNRFLVQRGI HDSFVTKFAE AMKKSLRVGN 

       370        380        390        400        410        420 
GFEEGTTQGP LINEKAVEKV EKHVNDAVAK GATVVTGGKR HQSGGNFFEP TLLSNVTRDM 

       430        440        450        460        470        480 
LCITEETFGP VAPVIKFDKE EEAVAIANAA DVGLAGYFYS QDPAQIWRVA EQLEVGMVGV 

       490        500        510        520 
NEGLISSVEC PFGGVKQSGL GREGSKYGID EYLEVKYVCY GGL 

P51650 in FASTA format

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