ID   MAOX_VITVI              Reviewed;         591 AA.
AC   P51615;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-NOV-2008, entry version 47.
DE   RecName: Full=NADP-dependent malic enzyme;
DE            Short=NADP-ME;
DE            EC=1.1.1.40;
OS   Vitis vinifera (Grape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Vitales; Vitaceae; Vitis.
OX   NCBI_TaxID=29760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Thompson; TISSUE=Fruit;
RX   MEDLINE=95232181; PubMed=7716227; DOI=10.1104/pp.107.3.1009;
RA   Franke K.E., Adams D.O.;
RT   "Cloning of a full-length cDNA for malic enzyme (EC 1.1.1.40) from
RT   grape berries.";
RL   Plant Physiol. 107:1009-1010(1995).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = pyruvate + CO(2) +
CC       NADPH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; L34836; AAA67087.1; -; mRNA.
DR   HSSP; P40927; 1GQ2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004473; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Metal-binding; NADP; Oxidoreductase.
FT   CHAIN         1    591       NADP-dependent malic enzyme.
FT                                /FTId=PRO_0000160204.
FT   NP_BIND     335    351       NADP (By similarity).
FT   ACT_SITE    139    139       Proton donor (By similarity).
FT   ACT_SITE    210    210       Proton acceptor (By similarity).
FT   METAL       282    282       Divalent metal cation (By similarity).
FT   METAL       283    283       Divalent metal cation (By similarity).
FT   METAL       306    306       Divalent metal cation (By similarity).
FT   BINDING     192    192       NAD (By similarity).
FT   BINDING     306    306       NAD (By similarity).
FT   BINDING     447    447       NAD (By similarity).
FT   SITE        306    306       Important for activity (By similarity).
SQ   SEQUENCE   591 AA;  65227 MW;  FD78B0976A7C4744 CRC64;
     MESTLKDIRD GASVLDLDPK ATVGGGVEDL YGEDFATEDQ LVTPWTVSVA SGYSLLRDPR
     HNKGLAFNDK ERDAHYLCGL LPPVVSTQEL QERKLMNSIR QYQVPLQKYM AMMDLQERNE
     RLFYKLLIDN VEELLPVVYT PTVGEACQKY GSIFRRPQGL YISLKEKGKI LEVLKNWPER
     RIQVIVVTDG ERILGLGDLG CQGMGIPVGK LSLYTALGGV RPSACLPITI DVGTNNEKLL
     ANEFYIGLKQ RRATGKEYSE FLQEFMSPVK QNYGEKVLIQ FEDFANHNAF DLLAKYGTTH
     LAFNDDIQGT ASVVLAGIVS ALRLLGGTLA DHKFLFLGAG EAGTGIAELI ALEMSKQTKC
     PIEETRKKIW LVDSKGLIVG SRKDSLQQFK KPWAHEHEPV KDLLDAVKVI KPTVLIGSSG
     VGKAFTKEVI EAMASCNEKP LILALSNPTS QSECTAEEAY TWTQGRAIFA SGSPFDPVEY
     NGKTFVPGQA NNAYIFPGLG MGLVISGAIR VHDEMLLAAS EALARQVTQE NFDKGLIYPP
     FSNIRKISAH IAANVAAKAY ELGLATRLPQ PENLVKYAES CMYSPVYRSY R
//