NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=New Zealand white;
TISSUE=Liver;
PubMed=7751825 [NCBI, ExPASy, EBI, Israel, Japan]
Kai M., Eto T., Kondo K., Setoguchi Y., Higashi S., Maeda Y., Setoguchi T.;
"Synchronous circadian rhythms of mRNA levels and activities of cholesterol 7 alpha-hydroxylase in the rabbit and rat.";
J. Lipid Res. 36:367-374(1995).

Comments

CATALYTIC ACTIVITY: Cholesterol + NADPH + O₂ = 7-alpha-hydroxycholesterol + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Lipid metabolism; bile acid biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L10754; AAA74382.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I46701; I46701.

3D structure databases

ModBase

P51542.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

P51542.

Phylogenomic databases

HOVERGEN

P51542; -.

Other

ProtoNet

P51542.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol metabolism; Endoplasmic reticulum; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Steroid metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 501`	`501`	`Cytochrome P450 7A1.`	`PRO_0000051904`
`METAL`	`441 441`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 501 AA [This is the length of the unprocessed precursor]

Molecular weight: 58090 Da [This is the MW of the unprocessed precursor]

CRC64: FEF1247B151AA6B1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MITIFWIWGI CLSVCCCLWL ILGLRRRRMG EPPLEKGWIP YLGCALQFGA NPLDFLRANQ 

        70         80         90        100        110        120 
RKYGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HFTTSAKAFG HRSIDPRDGN 

       130        140        150        160        170        180 
TTENINNTFN KTLQGDALIS LTDAMMENLQ LTLRRPEPKS RAWVTEGMYS FCYRVMFEAG 

       190        200        210        220        230        240 
YLTLFGRELT RQDAQRAFIL NSLEDFKQFD KVFPALVAGL PIHIFMTAHN AREKLAEGLK 

       250        260        270        280        290        300 
HDNLRTRDHI SELIRLRMFL NDTLSTFDAM EKAKTHLAIL WASQANTIPA TFWSLFHMMR 

       310        320        330        340        350        360 
SSEALKAATE EVNKALEDAD QQINFEGKPI HLNQTQLNDM PVLDSIIKES LRLSSASLNI 

       370        380        390        400        410        420 
RTAKEDFTLH LEDGSYNIRK DDIIALYPQL MHLDPEIYPD PMTFKYDRYL DENRKTKTTF 

       430        440        450        460        470        480 
YSKGLKLKYY YMPFGSGATI CPGRLFAIQE IKQFLILMLS YFELEFVDSH VKCPPLDQSR 

       490        500 
AGLGILPPLN DIEFKYKFKH L

P51542 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools