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UniProtKB/Swiss-Prot entry P51130


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UCRI_BRAJA
Primary accession number P51130
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 59)
Name and origin of the protein
Protein name Ubiquinol-cytochrome c reductase iron-sulfur subunit
Synonyms EC 1.10.2.2
Rieske iron-sulfur protein
RISP
Gene name
Name: petA
Synonyms: fbcF
OrderedLocusNames: blr2485
From
Bradyrhizobium japonicum [TaxID: 375] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Bradyrhizobiaceae; Bradyrhizobium.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1016/0092-8674(89)90137-2; PubMed=2541921 [NCBI, ExPASy, EBI, Israel, Japan]
Thoeny-Meyer L., Stax D., Hennecke H.;
"An unusual gene cluster for the cytochrome bc1 complex in Bradyrhizobium japonicum and its requirement for effective root nodule symbiosis.";
Cell 57:683-697(1989).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).
Comments
  • FUNCTION: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • CATALYTIC ACTIVITY: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
  • COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
  • SUBUNIT: The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
  • SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein.
  • DEVELOPMENTAL STAGE: Required for nitrogen fixation in root nodules on soybeans, but not for aerobic growth. It seems however to be expressed under many growth conditions (aerobic, microaerobic and in nodule bacteroids).
  • MISCELLANEOUS: The Rieske protein is a high potential 2Fe-2S protein.
  • SIMILARITY: Contains 1 Rieske domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J03176; AAA26199.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000040; BAC47750.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A32382; A32382.
RefSeq NP_769125.1; -.
3D structure databases
HSSP P13272; 1RIE. [HSSP ENTRY / PDB]
ModBase P51130.
Enzyme and pathway databases
BioCyc BJAP224911:BLR2485-MON; -.
Family and domain databases
InterPro IPR014349; Rieske.
IPR005805; Rieske_C.
IPR006317; Rieske_proteo.
IPR005806; Rieske_reg.
IPR006311; Tat.
Graphical view of domain structure.
Gene3D G3DSA:2.102.10.10; Rieske_reg; 1.
PANTHER PTHR10134; Rieske; 1.
Pfam PF00355; Rieske; 1.
Pfam graphical view of domain structure.
PRINTS PR00162; RIESKE.
TIGRFAMs TIGR01416; Rieske_proteo; 1.
TIGR01409; TAT_signal_seq; 1.
PROSITE PS51296; RIESKE; 1.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P51130.
Genome annotation databases
GeneID 1050391; -.
GenomeReviews BA000040_GR; blr2485.
KEGG bja:blr2485; -.
NMPDR fig|224911.1.peg.2485; -.
Phylogenomic databases
HOGENOM P51130; -.
Genome annotation databases
CMR P51130; blr2485.
Other
ProtoNet P51130.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; Cell membrane; Complete proteome; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   176  176     Ubiquinol-cytochrome c reductase iron-sulfur subunit. PRO_0000127759
TRANSMEM   15    36  22     Potential. 
DOMAIN   87   174  88     Rieske. 
METAL   119   119        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   121   121        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
METAL   138   138        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   141   141        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
DISULFID   124   140        By similarity. 
Sequence information
Length: 176 AA [This is the length of the unprocessed precursor] Molecular weight: 18823 Da [This is the MW of the unprocessed precursor] CRC64: 19C636F520E3FBF5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTTASSADHP TRRDFLFVAT GAAAAVGGAA ALWPFISQMN PDASTIAAGA PIEVDLSPIA 

        70         80         90        100        110        120 
EGQDIKVFWR GKPIYISHRT KKQIDEARAV NVASLPDPQS DEARVKSGHE QWLVVIGICT 

       130        140        150        160        170 
HLGCIPIAHE GNYDGFFCPC HGSQYDSSGR IRQGPAPANL PVPPYQFVSD TKIQIG 

P51130 in FASTA format

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