ID   LDOX_PETHY              Reviewed;         430 AA.
AC   P51092;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   04-NOV-2008, entry version 47.
DE   RecName: Full=Leucoanthocyanidin dioxygenase;
DE            Short=LDOX;
DE            Short=Leucocyanidin oxygenase;
DE            EC=1.14.11.19;
DE   AltName: Full=Leucoanthocyanidin hydroxylase;
GN   Name=ANT17;
OS   Petunia hybrida (Petunia).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX   NCBI_TaxID=4102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Violet 26; TISSUE=Corolla;
RX   MEDLINE=93363921; PubMed=8358035; DOI=10.1007/BF00027374;
RA   Weiss D., van der Luit A.H., Kroon J.T.M., Mol J.N.M., Kooter J.M.;
RT   "The petunia homologue of the Antirrhinum majus candi and Zea mays A2
RT   flavonoid genes; homology to flavanone 3-hydroxylase and ethylene-
RT   forming enzyme.";
RL   Plant Mol. Biol. 22:893-897(1993).
CC   -!- FUNCTION: Oxidation of leucoanthocyanidins into anthocyanidins.
CC   -!- CATALYTIC ACTIVITY: Leucocyanidin + 2-oxoglutarate + O(2) = cis-
CC       and trans-dihydroquercetins + succinate + CO(2) + H(2)O.
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 ascorbate molecule per subunit (By similarity).
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in corollas and at
CC       lower levels in anthers.
CC   -!- INDUCTION: By gibberellic acid (GA).
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; X70786; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; S36233; S36233.
DR   HSSP; Q96323; 1GP5.
DR   SMR; P51092; 11-353.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0050589; F:leucocyanidin oxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; 2OG-FeII_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    430       Leucoanthocyanidin dioxygenase.
FT                                /FTId=PRO_0000067303.
FT   METAL       236    236       Iron (By similarity).
FT   METAL       238    238       Iron (By similarity).
FT   METAL       292    292       Iron (By similarity).
SQ   SEQUENCE   430 AA;  48433 MW;  72AB3DA2ACA7D22C CRC64;
     MVNAVVTTPS RVESLAKSGI QAIPKEYVRP QEELNGIGNI FEEEKKDEGP QVPTIDLKEI
     DSEDKEIREK CHQLKKAAME WGVMHLVNHG ISDELINRVK VAGETFFDQP VEEKEKYAND
     QANGNVQGYG SKLANSACGQ LEWEDYFFHC AFPEDKRDLS IWPKNPTDYT PATSEYAKQI
     RALATKILTV LSIGLGLEEG RLEKEVGGME DLLLQMKINY YPKCPQPELA LGVEAHTDVS
     ALTFILHNMV PGLQLFYEGQ WVTAKCVPNS IIMHIGDTIE ILSNGKYKSI LHRGVVNKEK
     VRFSWAIFCE PPKEKIILKP LPETVTEAEP PRFPPRTFAQ HMAHKLFRKD DKDAAVEHKV
     FNEDELDTAA EHKVLKKDNQ DAVAENKDIK EDEQCGPAEH KDIKEDGQGA AAENKVFKEN
     NQDVAAEESK
//