NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Violet 26;
TISSUE=Corolla;
DOI=10.1007/BF00027374; PubMed=8358035 [NCBI, ExPASy, EBI, Israel, Japan]
Weiss D., van der Luit A.H., Kroon J.T.M., Mol J.N.M., Kooter J.M.;
"The petunia homologue of the Antirrhinum majus candi and Zea mays A2 flavonoid genes; homology to flavanone 3-hydroxylase and ethylene-forming enzyme.";
Plant Mol. Biol. 22:893-897(1993).

Comments

FUNCTION: Oxidation of leucoanthocyanidins into anthocyanidins.
CATALYTIC ACTIVITY: Leucocyanidin + 2-oxoglutarate + O₂ = cis- and trans-dihydroquercetins + succinate + CO₂ + H₂O.
COFACTOR: Binds 1 iron ion per subunit (By similarity).
COFACTOR: Binds 1 ascorbate molecule per subunit (By similarity).
PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis .
TISSUE SPECIFICITY: Predominantly expressed in corollas and at lower levels in anthers.
INDUCTION: By gibberellic acid (GA).
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X70786; -; NOT_ANNOTATED_CDS; mRNA.

[EMBL / GenBank / DDBJ]

PIR

S36233; S36233.

3D structure databases

HSSP

Q96323; 1GP5. [HSSP ENTRY / PDB]

SMR

P51092; 11-353.

ModBase

P51092.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 430`	`430`	`Leucoanthocyanidin dioxygenase.`	`PRO_0000067303`
`METAL`	`236 236`		`Iron (By similarity).`
`METAL`	`238 238`		`Iron (By similarity).`
`METAL`	`292 292`		`Iron (By similarity).`

Sequence information

Length: 430 AA [This is the length of the unprocessed precursor]

Molecular weight: 48433 Da [This is the MW of the unprocessed precursor]

CRC64: 72AB3DA2ACA7D22C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVNAVVTTPS RVESLAKSGI QAIPKEYVRP QEELNGIGNI FEEEKKDEGP QVPTIDLKEI 

        70         80         90        100        110        120 
DSEDKEIREK CHQLKKAAME WGVMHLVNHG ISDELINRVK VAGETFFDQP VEEKEKYAND 

       130        140        150        160        170        180 
QANGNVQGYG SKLANSACGQ LEWEDYFFHC AFPEDKRDLS IWPKNPTDYT PATSEYAKQI 

       190        200        210        220        230        240 
RALATKILTV LSIGLGLEEG RLEKEVGGME DLLLQMKINY YPKCPQPELA LGVEAHTDVS 

       250        260        270        280        290        300 
ALTFILHNMV PGLQLFYEGQ WVTAKCVPNS IIMHIGDTIE ILSNGKYKSI LHRGVVNKEK 

       310        320        330        340        350        360 
VRFSWAIFCE PPKEKIILKP LPETVTEAEP PRFPPRTFAQ HMAHKLFRKD DKDAAVEHKV 

       370        380        390        400        410        420 
FNEDELDTAA EHKVLKKDNQ DAVAENKDIK EDEQCGPAEH KDIKEDGQGA AAENKVFKEN 

       430 
NQDVAAEESK

P51092 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools