[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Leaf; DOI=10.1104/pp.103.3.1015; PubMed=8022923 [NCBI, ExPASy, EBI, Israel, Japan] Davies K.M.; "A Malus cDNA with homology to the Antirrhinum Candica and Zea A2 genes."; Plant Physiol. 103:1015-1015(1993).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Fuji; TISSUE=Peelings; Lee J.-R., Hong S.-T., Yoo Y.G., Kim S.-R.; "Molecular cloning and expression of anthocyanin biosynthesis genes from 'Fuji apple'."; Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Oxidation of leucoanthocyanidins into anthocyanidins.
CATALYTIC ACTIVITY: Leucocyanidin + 2-oxoglutarate + O₂ = cis- and trans-dihydroquercetins + succinate + CO₂ + H₂O.
COFACTOR: Binds 1 iron ion per subunit (By similarity).
COFACTOR: Binds 1 ascorbate molecule per subunit (By similarity).
PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis .
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X71360; CAA50498.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF117269; AAD26205.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S33144; S33144.

3D structure databases

HSSP

Q96323; 1GP6. [HSSP ENTRY / PDB]

SMR

P51091; 10-354.

ModBase

P51091.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0031418;	Molecular function: L-ascorbic acid binding (inferred from electronic annotation from UniProtKB-KW).
GO:0050589;	Molecular function: leucocyanidin oxygenase activity (inferred from electronic annotation from EC).
GO:0016702;	Molecular function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen (inferred from electronic annotation from UniProtKB-KW).
GO:0009813;	Biological process: flavonoid biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

ProtoNet

P51091.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 357`	`357`	`Leucoanthocyanidin dioxygenase.`	`PRO_0000067301`
`METAL`	`236 236`		`Iron (By similarity).`
`METAL`	`238 238`		`Iron (By similarity).`
`METAL`	`292 292`		`Iron (By similarity).`

Sequence information

Length: 357 AA [This is the length of the unprocessed precursor]

Molecular weight: 40332 Da [This is the MW of the unprocessed precursor]

CRC64: FA5D97C25267B3E6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVSSDSVNSR VETLAGSGIS TIPKEYIRPK DELVNIGDIF EQEKNNEGPQ VPTIDLKEIE 

        70         80         90        100        110        120 
SDNEKVRAKC REKLKKAAVD WGVMHLVNHG ISDELMDKVR KAGKAFFDLP IEQKEKYAND 

       130        140        150        160        170        180 
QASGKIQGYG SKLANNASGQ LEWEDYFFHC VYPEDKRDLS IWPQTPADYI EATAEYAKQL 

       190        200        210        220        230        240 
RELATKVLKV LSLGLGLDEG RLEKEVGGLE ELLLQMKINY YPKCPQPELA LGVEAHTDVS 

       250        260        270        280        290        300 
ALTFILHNMV PGLQLFYEGK WVTAKCVPNS IVMHIGDTLE ILSNGKYKSI LHRGMVNKEK 

       310        320        330        340        350 
VRISWAVFCE PPKEKIILKP LPETVSEDEP AMFPPRTFAE HIQHKLFRKS QEALLPK

P51091 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools