ID   DHSA_COXBU              Reviewed;         587 AA.
AC   P51054;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-NOV-2008, entry version 68.
DE   RecName: Full=Succinate dehydrogenase flavoprotein subunit;
DE            EC=1.3.99.1;
GN   Name=sdhA; OrderedLocusNames=CBU_1401;
OS   Coxiella burnetii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC   Coxiellaceae; Coxiella.
OX   NCBI_TaxID=777;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Nine Mile;
RX   MEDLINE=95212926; PubMed=7698664; DOI=10.1016/0378-1119(94)00888-Y;
RA   Heinzen R.A., Mo Y.-Y., Robertson S.J., Mallavia L.P.;
RT   "Characterization of the succinate dehydrogenase-encoding gene cluster
RT   (sdh) from the rickettsia Coxiella burnetii.";
RL   Gene 155:27-34(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nine Mile phase I / RSA 493;
RX   MEDLINE=22608657; PubMed=12704232; DOI=10.1073/pnas.0931379100;
RA   Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E.,
RA   Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J.,
RA   DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J.,
RA   Khouri H.M., Lee K.H., Carty H.A., Scanlan D., Heinzen R.A.,
RA   Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F.;
RT   "Complete genome sequence of the Q-fever pathogen, Coxiella
RT   burnetii.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003).
CC   -!- CATALYTIC ACTIVITY: Succinate + acceptor = fumarate + reduced
CC       acceptor.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC   -!- SUBUNIT: Part of an enzyme complex containing four subunits: a
CC       flavoprotein, an iron-sulfur protein, cytochrome b-556 and a
CC       hydrophobic protein.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane
CC       protein; Cytoplasmic side (By similarity).
CC   -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family.
CC       FRD/SDH subfamily.
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DR   EMBL; L33409; AAA74133.1; -; Genomic_DNA.
DR   EMBL; AE016828; AAO90900.1; -; Genomic_DNA.
DR   PIR; I40849; I40849.
DR   RefSeq; NP_820386.1; -.
DR   HSSP; P10444; 1NEK.
DR   GeneID; 1209307; -.
DR   GenomeReviews; AE016828_GR; CBU_1401.
DR   KEGG; cbu:CBU_1401; -.
DR   TIGR; CBU_1401; -.
DR   HOGENOM; P51054; -.
DR   BioCyc; CBUR227377:CBU_1401-MON; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0000104; F:succinate dehydrogenase activity; IEA:EC.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   InterPro; IPR003953; FAD_bind2_N.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR003952; FRD_SDH_FAD_BS.
DR   InterPro; IPR004112; Fum_Rdtase/Succ_DHase_flav_C.
DR   InterPro; IPR011281; Succ_DHase_flav_su_fwd.
DR   InterPro; IPR014006; Succ_Dhase_frdA_Gneg.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   Pfam; PF02910; Succ_DH_flav_C; 1.
DR   PRINTS; PR00368; FADPNR.
DR   TIGRFAMs; TIGR01816; sdhA_forward; 1.
DR   TIGRFAMs; TIGR01812; sdhA_frdA_Gneg; 1.
DR   PROSITE; PS00504; FRD_SDH_FAD_BINDING; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Complete proteome;
KW   Electron transport; FAD; Flavoprotein; Membrane; Oxidoreductase;
KW   Transport; Tricarboxylic acid cycle.
FT   CHAIN         1    587       Succinate dehydrogenase flavoprotein
FT                                subunit.
FT                                /FTId=PRO_0000158651.
FT   NP_BIND      39     54       FAD (By similarity).
FT   ACT_SITE    288    288       Proton acceptor (By similarity).
FT   BINDING     244    244       Substrate (By similarity).
FT   BINDING     256    256       Substrate (By similarity).
FT   BINDING     355    355       Substrate (By similarity).
FT   BINDING     400    400       Substrate (By similarity).
FT   MOD_RES      47     47       Tele-8alpha-FAD histidine (By
FT                                similarity).
SQ   SEQUENCE   587 AA;  65438 MW;  37B264ECC98D3DB6 CRC64;
     MSSIRVKQYD ALIVGAGGAG LRAALEMAQS RQYKVAVVSK VFPTRSHTVS AQGGIAAALG
     NVVPDKPIWH MFDTVKGSDY LGDQDAIQYM CEQAPPSVYE LEHYGLPFSR LDDGRIYQRA
     FGGHTRDFGK EMARRTCACA DRTGHAMLHT LYQKNVEAGT HFYYEWYGID LVRGAQGGIA
     GMIAMNMETS ELVFFKSRAT IFATGGAGRI YETTSNAYTN TGDGIGMVLR AGLPVQDMEF
     WQFHPTGIYG VGCLITEGAR GEGGYLINKD GERFMERYSP HLKDLDCRDV VARSILQEVM
     AGGGVGPKKD HVLLKLDHLG EKVLRERLPG IIELSEKFAN VDITKEPIPI LPTCHYMMGG
     IPTNIHGQAL TVDENGKDQI IEGLFAAGEC ACVSVHGANR LGTNSLLDLV VFGRAIGLHL
     EEALKTELKH RSENPDDIDA AIARLKRWEK PNNVENPALL RQEMRKAMSE DFGVFREEQK
     MKQGLERLQK LNERLQRAKL TDTSRTFNNA RIEALELDNL MEVSYATAVS AQQRTESRGA
     HSRYDYKERD DANWLKHTVY FRDGHIAYRP VNMKPKGMDP FPPKSRD
//