NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=195;
DOI=10.1016/0923-2508(96)81052-7; PubMed=7569323 [NCBI, ExPASy, EBI, Israel, Japan]
Dartois V., Phalip V., Schmitt P., Divies C.;
"Purification, properties and DNA sequence of the D-lactate dehydrogenase from Leuconostoc mesenteroides subsp. cremoris.";
Res. Microbiol. 146:291-302(1995).

Comments

CATALYTIC ACTIVITY: (R)-lactate + NAD⁺ = pyruvate + NADH.
SUBUNIT: Homodimer.
SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L29327; AAA99506.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P30901; 2DLD. [HSSP ENTRY / PDB]

ModBase

P51011.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-13061; -.

Family and domain databases

InterPro

IPR006139; D-isomer_2_OHA_DHase.
IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00389; 2-Hacid_dh; 1.
PF02826; 2-Hacid_dh_C; 1.
Pfam graphical view of domain structure.

PROSITE

PS00065; D_2_HYDROXYACID_DH_1; 1.
PS00670; D_2_HYDROXYACID_DH_2; FALSE_NEG.
PS00671; D_2_HYDROXYACID_DH_3; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 331`	`331`	`D-lactate dehydrogenase.`	`PRO_0000075957`
`ACT_SITE`	`235 235`		`By similarity.`
`ACT_SITE`	`264 264`		`By similarity.`
`ACT_SITE`	`296 296`		`Proton donor (By similarity).`

Sequence information

Length: 331 AA [This is the length of the unprocessed precursor]

Molecular weight: 36316 Da [This is the MW of the unprocessed precursor]

CRC64: 0A63C462303672D8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKIFAYGIRD DEKPSLEEWK AANPEIEVDY TQELLTPETA KLAEGSDSAV VYQQLDYTRE 

        70         80         90        100        110        120 
TLTALANVGV TNLSLRNVGT DNIDFDAARE FNFNISNVPV YSPNAIAEHS MLQLSRLLRR 

       130        140        150        160        170        180 
TKALDAKIAK RDLRWAPTTG REMRMQTVGV IGTGHIGRVA INILKGFGAK VIAYDKYPNA 

       190        200        210        220        230        240 
ELQAEGLYVD TLDELYAQAD AISLYVPGVP ENHHLINADA IAKMKDGVVI MNAARGNLMD 

       250        260        270        280        290        300 
IDAIIDGLNS GKISDFGMDV YENEVACSMK IGLVKNSPDA KIADLIAREN VMITPHTAFY 

       310        320        330 
TTKAVLEMVH QSFDAAVAFA KGEKPAIAVE Y

P51011 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools