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UniProtKB/Swiss-Prot entry P50735


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GUDB_BACSU
Primary accession number P50735
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 58)
Name and origin of the protein
Protein name NAD-specific glutamate dehydrogenase
Synonyms NAD-GDH
EC 1.4.1.2
Gene name
Name: gudB
Synonyms: ypcA
OrderedLocusNames: BSU22960
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168 / Marburg;
PubMed=8760912 [NCBI, ExPASy, EBI, Israel, Japan]
Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.;
"Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome.";
Microbiology 142:2005-2016(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[3]
CHARACTERIZATION.
PubMed=9829940 [NCBI, ExPASy, EBI, Israel, Japan]
Belitsky B.R., Sonenshein A.L.;
"Role and regulation of Bacillus subtilis glutamate dehydrogenase genes.";
J. Bacteriol. 180:6298-6305(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L47648; AAC83953.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99115; CAB14212.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR G69933; G69933.
RefSeq NP_390177.1; -.
3D structure databases
HSSP P80319; 1GTM. [HSSP ENTRY / PDB]
ModBase P50735.
Enzyme and pathway databases
BioCyc BSUB224308:BSU2295-MON; -.
Organism-specific databases
SubtiList BG11435; gudB. [Micado]
Family and domain databases
InterPro IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11606:SF2; GLFV_DH; 1.
Pfam PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000185; Glu_DH; 1.
PRINTS PR00082; GLFDHDRGNASE.
PROSITE PS00074; GLFV_DEHYDROGENASE; 1.
BLOCKS P50735.
Genome annotation databases
GeneID 938975; -.
GenomeReviews AL009126_GR; BSU22960.
KEGG bsu:BSU22960; -.
NMPDR fig|224308.1.peg.2300; -.
Phylogenomic databases
HOGENOM P50735; -.
Genome annotation databases
CMR P50735; BSU22960.
Other
ProtoNet P50735.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   426  426     NAD-specific glutamate dehydrogenase. PRO_0000182736
ACT_SITE   118   118        By similarity. 
VARIANT   96    98  3     Missing (in spontaneous mutant gudB1; restores activity). 
Sequence information
Length: 426 AA [This is the length of the unprocessed precursor] Molecular weight: 47212 Da [This is the MW of the unprocessed precursor] CRC64: 5624F2DE93333E86 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAADRNTGHT EEDKLDVLKS TQTVIHKALE KLGYPEEVYE LLKEPMRLLT VKIPVRMDDG 

        70         80         90        100        110        120 
SVKIFTGYRA HNDSVGPTKG GIRFHPNVTE KEVKAVKALS IWMSLKCGII DLPYGGGKGG 

       130        140        150        160        170        180 
IVCDPRDMSF RELERLSRGY VRAISQIVGP TKDVPAPDVF TNSQIMAWMM DEYSRIDEFN 

       190        200        210        220        230        240 
SPGFITGKPL VLGGSHGRES ATAKGVTICI KEAAKKRGID IKGARVVVQG FGNAGSYLAK 

       250        260        270        280        290        300 
FMHDAGAKVV GISDAYGGLY DPEGLDIDYL LDRRDSFGTV TKLFNDTITN QELLELDCDI 

       310        320        330        340        350        360 
LVPAAIENQI TEENAHNIRA KIVVEAANGP TTLEGTKILS DRDILLVPDV LASAGGVTVS 

       370        380        390        400        410        420 
YFEWVQNNQG FYWSEEEVEE KLEKMMVKSF NNIYEMANNR RIDMRLAAYM VGVRKMAEAS 


RFRGWI 

P50735 in FASTA format

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