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UniProtKB/Swiss-Prot entry P50216

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IDH_CORGL
Primary accession number P50216
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 53)
Name and origin of the protein
Protein name Isocitrate dehydrogenase [NADP]
Synonyms IDH
EC 1.1.1.42
Oxalosuccinate decarboxylase
Gene name
Name: icd
OrderedLocusNames: Cgl0664, cg0766
From
Corynebacterium glutamicum (Brevibacterium flavum) [TaxID: 1718] [HAMAP proteome]
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Corynebacterineae; Corynebacteriaceae; Corynebacterium.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
PubMed=7836312 [NCBI, ExPASy, EBI, Israel, Japan]
Eikmanns B.J., Rittmann D., Sahm H.;
"Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme.";
J. Bacteriol. 177:774-782(1995).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
Nakagawa S.;
"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032.";
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
DOI=10.1016/S0168-1656(03)00154-8; PubMed=12948626 [NCBI, ExPASy, EBI, Israel, Japan]
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B., Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.;
"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.";
J. Biotechnol. 104:5-25(2003).
[4]
 X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) IN COMPLEX WITH MAGNESIUM, AND SUBUNIT.
DOI=10.1002/prot.20867; PubMed=16416443 [NCBI, ExPASy, EBI, Israel, Japan]
Imabayashi F., Aich S., Prasad L., Delbaere L.T.;
"Substrate-free structure of a monomeric NADP isocitrate dehydrogenase: an open conformation phylogenetic relationship of isocitrate dehydrogenase.";
Proteins 63:100-112(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X71489; CAA50590.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000036; BAB98057.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BX927149; CAF19369.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR I40719; I40719.
RefSeq NP_599896.1; -.
YP_224955.1; -.
3D structure databases
PDB
2B0T; X-ray; 1.75 A; A=1-738.[ExPASy / RCSB / EBI]
PDBsum 2B0T; -.
ModBase P50216.
Enzyme and pathway databases
BioCyc CGLU196627-1:CG0766-MON; -.
Ontologies
GO
GO:0004450; Molecular function: isocitrate dehydrogenase (NADP+) activity (inferred from electronic annotation from InterPro).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006097; Biological process: glyoxylate cycle (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006099; Biological process: tricarboxylic acid cycle (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR004436; IsoCit_DHase_NAD_mono.
Graphical view of domain structure.
Pfam PF03971; IDH; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF009407; IDH_monmr; 1.
TIGRFAMs TIGR00178; monomer_idh; 1.
Genome annotation databases
GeneID 1018663; -.
3345662; -.
GenomeReviews BA000036_GR; Cgl0664.
BX927147_GR; cg0766.
KEGG cgb:cg0766; -.
cgl:NCgl0634; -.
Phylogenomic databases
HOGENOM P50216; -.
Genome annotation databases
CMR P50216; Cgl0664.
Other
ProtoNet P50216.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Glyoxylate bypass; Magnesium; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   738  738     Isocitrate dehydrogenase [NADP]. PRO_0000083595
NP_BIND   80    85  6     NADP (By similarity). 
NP_BIND   580   581  2     NADP (By similarity). 
NP_BIND   596   598  3     NADP (By similarity). 
REGION   130   137  8     Substrate binding (By similarity). 
METAL   346   346        Magnesium or manganese. 
METAL   544   544        Magnesium or manganese. 
METAL   548   548        Magnesium or manganese. 
BINDING   133   133        NADP (By similarity). 
BINDING   143   143        Substrate (By similarity). 
BINDING   543   543        Substrate (By similarity). 
BINDING   585   585        NADP (By similarity). 
BINDING   645   645        NADP (By similarity). 
SITE   253   253  1     Critical for catalysis (By similarity). 
SITE   416   416  1     Critical for catalysis (By similarity). 
STRAND   3     8  6      
HELIX   11    29  19      
TURN   30    32  3      
STRAND   34    39  6      
HELIX   42    47  6      
HELIX   51    53  3      
TURN   56    58  3      
HELIX   63    70  8      
STRAND   77    80  4      
HELIX   88   100  13      
HELIX   115   125  11      
STRAND   128   130  3      
HELIX   132   136  5      
STRAND   141   144  4      
HELIX   147   155  9      
STRAND   170   172  3      
STRAND   175   178  4      
HELIX   179   182  4      
STRAND   184   187  4      
STRAND   192   199  8      
STRAND   205   214  10      
STRAND   219   225  7      
HELIX   227   244  18      
STRAND   247   250  4      
TURN   254   256  3      
HELIX   260   272  13      
HELIX   274   286  13      
HELIX   294   302  9      
HELIX   308   321  14      
STRAND   327   329  3      
HELIX   330   332  3      
STRAND   342   344  3      
HELIX   345   354  10      
TURN   355   357  3      
STRAND   358   360  3      
STRAND   366   372  7      
HELIX   376   392  17      
TURN   397   399  3      
STRAND   405   407  3      
TURN   410   412  3      
HELIX   414   417  4      
HELIX   419   421  3      
STRAND   422   424  3      
STRAND   427   434  8      
STRAND   440   446  7      
STRAND   451   457  7      
HELIX   459   476  18      
STRAND   480   483  4      
HELIX   489   502  14      
STRAND   512   515  4      
HELIX   517   529  13      
STRAND   535   538  4      
HELIX   540   555  16      
STRAND   558   560  3      
STRAND   562   567  6      
STRAND   572   575  4      
HELIX   583   592  10      
HELIX   600   617  18      
HELIX   620   638  19      
HELIX   652   669  18      
HELIX   674   700  27      
STRAND   711   713  3      
HELIX   716   723  8      
HELIX   727   735  9      
Sequence information
Length: 738 AA [This is the length of the unprocessed precursor] Molecular weight: 80081 Da [This is the MW of the unprocessed precursor] CRC64: EAC4C5FA38C33F63 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAKIIWTRTD EAPLLATYSL KPVVEAFAAT AGIEVETRDI SLAGRILAQF PERLTEDQKV 

        70         80         90        100        110        120 
GNALAELGEL AKTPEANIIK LPNISASVPQ LKAAIKELQD QGYDIPELPD NATTDEEKDI 

       130        140        150        160        170        180 
LARYNAVKGS AVNPVLREGN SDRRAPIAVK NFVKKFPHRM GEWSADSKTN VATMDANDFR 

       190        200        210        220        230        240 
HNEKSIILDA ADEVQIKHIA ADGTETILKD SLKLLEGEVL DGTVLSAKAL DAFLLEQVAR 

       250        260        270        280        290        300 
AKAEGILFSA HLKATMMKVS DPIIFGHVVR AYFADVFAQY GEQLLAAGLN GENGLAAILS 

       310        320        330        340        350        360 
GLESLDNGEE IKAAFEKGLE DGPDLAMVNS ARGITNLHVP SDVIVDASMP AMIRTSGHMW 

       370        380        390        400        410        420 
NKDDQEQDTL AIIPDSSYAG VYQTVIEDCR KNGAFDPTTM GTVPNVGLMA QKAEEYGSHD 

       430        440        450        460        470        480 
KTFRIEADGV VQVVSSNGDV LIEHDVEAND IWRACQVKDA PIQDWVKLAV TRSRLSGMPA 

       490        500        510        520        530        540 
VFWLDPERAH DRNLASLVEK YLADHDTEGL DIQILSPVEA TQLSIDRIRR GEDTISVTGN 

       550        560        570        580        590        600 
VLRDYNTDLF PILELGTSAK MLSVVPLMAG GGLFETGAGG SAPKHVQQVQ EENHLRWDSL 

       610        620        630        640        650        660 
GEFLALAESF RHELNNNGNT KAGVLADALD KATEKLLNEE KSPSRKVGEI DNRGSHFWLT 

       670        680        690        700        710        720 
KFWADELAAQ TEDADLAATF APVAEALNTG AADIDAALLA VQGGATDLGG YYSPNEEKLT 

       730 
NIMRPVAQFN EIVDALKK
P50216 in FASTA format

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