ID   IDH_SPHYA               Reviewed;         406 AA.
AC   P50215;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-NOV-2008, entry version 47.
DE   RecName: Full=Isocitrate dehydrogenase [NADP];
DE            Short=IDH;
DE            EC=1.1.1.42;
DE   AltName: Full=Oxalosuccinate decarboxylase;
DE   AltName: Full=NADP(+)-specific ICDH;
DE   AltName: Full=IDP;
GN   Name=icd; Synonyms=idhA;
OS   Sphingomonas yanoikuyae.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=13690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B1;
RX   MEDLINE=96186949; PubMed=8626059; DOI=10.1016/0378-1119(95)00732-6;
RA   Wang Y., Lau P.C.K.;
RT   "Sequence and expression of an isocitrate dehydrogenase-encoding gene
RT   from a polycyclic aromatic hydrocarbon oxidizer, Sphingomonas
RT   yanoikuyae B1.";
RL   Gene 168:15-21(1996).
CC   -!- CATALYTIC ACTIVITY: Isocitrate + NADP(+) = 2-oxoglutarate + CO(2)
CC       + NADPH.
CC   -!- CATALYTIC ACTIVITY: Oxalosuccinate + NADP(+) = 2-oxoglutarate +
CC       CO(2) + NADPH.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family.
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DR   EMBL; U37523; AAC43640.1; -; Genomic_DNA.
DR   PIR; JC4600; JC4600.
DR   HSSP; P33198; 1LWD.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006102; P:isocitrate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR004790; IsoCit_DHase_NADP-dep_euk.
DR   InterPro; IPR001804; IsoCit_IM_DHase.
DR   Gene3D; G3DSA:3.40.718.10; IDH_IMDH; 1.
DR   PANTHER; PTHR11822; IDH_NADP_euk; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   PIRSF; PIRSF000108; IDH_NADP; 1.
DR   TIGRFAMs; TIGR00127; nadp_idh_euk; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   3: Inferred from homology;
KW   Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP;
KW   Oxidoreductase; Phosphoprotein; Tricarboxylic acid cycle.
FT   CHAIN         1    406       Isocitrate dehydrogenase [NADP].
FT                                /FTId=PRO_0000083567.
FT   NP_BIND      75     77       NADP (By similarity).
FT   NP_BIND     308    313       NADP (By similarity).
FT   REGION       94    100       Substrate binding (By similarity).
FT   METAL       250    250       Magnesium or manganese (By similarity).
FT   METAL       273    273       Magnesium or manganese (By similarity).
FT   BINDING      77     77       Substrate (By similarity).
FT   BINDING      82     82       NADP (By similarity).
FT   BINDING     109    109       Substrate (By similarity).
FT   BINDING     132    132       Substrate (By similarity).
FT   BINDING     258    258       NADP (By similarity).
FT   BINDING     326    326       NADP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   SITE        139    139       Critical for catalysis (By similarity).
FT   SITE        210    210       Critical for catalysis (By similarity).
FT   MOD_RES      94     94       Phosphoserine (By similarity).
SQ   SEQUENCE   406 AA;  45483 MW;  093F7E18303A89E7 CRC64;
     MAKIKVKNPV VEIDGDEMTR IIWEWIRERL ILPYLDVDLK YYDLSVEKRD ETSDQITIDA
     ANAIKEYGVG VKCATITPDE ARVEEFGLKK MWKSPNGTIR NILGGVVFRE PIVIKNVPRL
     VPGWTDPIVV GRHAFGDQYK ATDFKVPGAG TLTMKWVGTN GEELEYEVFE FPSAGVAMGM
     YNLDESIRDF AKASFNYGLN RGWPVYLSTK NTILKAYDGR FKDLFQEVFD AEFADKFKAA
     GIVYEHRLID DMVASALKWS GKFVWACKNY DGDVQSDTVA QGFGSLGLMT SVLLSPDGKT
     VEAEAAHGTV TRHYRQHQQG KATSTNPIAS IFAWTQGLSF RGKFDDTPDV VKFAETLEQV
     CIKTVEGGAM TKDLALLIGP DQAWMTTEQF FEAIRVNLEA EMAKWA
//