ID   FMO5_HUMAN              Reviewed;         533 AA.
AC   P49326;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-DEC-2008, entry version 78.
DE   RecName: Full=Dimethylaniline monooxygenase [N-oxide-forming] 5;
DE            EC=1.14.13.8;
DE   AltName: Full=Hepatic flavin-containing monooxygenase 5;
DE            Short=FMO 5;
DE   AltName: Full=Dimethylaniline oxidase 5;
GN   Name=FMO5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   MEDLINE=95177663; PubMed=7872795; DOI=10.1006/abbi.1995.1163;
RA   Overby L.H., Buckpitt A.R., Lawton M.P., Atta-Asafo-Adjei E.,
RA   Schulze J., Philpot R.M.;
RT   "Characterization of flavin-containing monooxygenase 5 (FMO5) cloned
RT   from human and guinea pig: evidence that the unique catalytic
RT   properties of FMO5 are not confined to the rabbit ortholog.";
RL   Arch. Biochem. Biophys. 317:275-284(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver, and Placenta;
RA   Dolphin C.T., Povey S., Shephard E.A., Smith R.L., Phillips I.R.;
RT   "Cloning, primary sequence and chromosomal localisation of human
RT   flavin-containing monooxygenase 5 (FMO5).";
RL   Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ALA-400 AND SER-506.
RG   NIEHS SNPs program;
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA   Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA   Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA   McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA   Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA   Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA   Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA   Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA   Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA   Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA   Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA   Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA   Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA   Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA   Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA   Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA   Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA   Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA   Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA   Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA   Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA   Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA   Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA   Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA   Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [5]
RP   VARIANT LEU-457.
RX   MEDLINE=22414999; PubMed=12527699; DOI=10.1124/dmd.31.2.187;
RA   Furnes B., Feng J., Sommer S.S., Schlenk D.;
RT   "Identification of novel variants of the flavin-containing
RT   monooxygenase gene family in African Americans.";
RL   Drug Metab. Dispos. 31:187-193(2003).
CC   -!- FUNCTION: In contrast with other forms of FMO it does not seem to
CC       be a drug-metabolizing enzyme.
CC   -!- CATALYTIC ACTIVITY: N,N-dimethylaniline + NADPH + O(2) = N,N-
CC       dimethylaniline N-oxide + NADP(+) + H(2)O.
CC   -!- COFACTOR: FAD.
CC   -!- SUBCELLULAR LOCATION: Microsome membrane. Endoplasmic reticulum
CC       membrane.
CC   -!- TISSUE SPECIFICITY: Expressed in fetal and adult liver.
CC   -!- SIMILARITY: Belongs to the FMO family.
CC   -!- WEB RESOURCE: Name=NIEHS-SNPs;
CC       URL="http://egp.gs.washington.edu/data/fmo5/";
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DR   EMBL; L37080; AAA67849.1; -; mRNA.
DR   EMBL; Z47553; CAA87633.1; -; mRNA.
DR   EMBL; AY902236; AAW69390.1; -; Genomic_DNA.
DR   EMBL; AL356378; CAH72648.1; -; Genomic_DNA.
DR   PIR; S51131; S51131.
DR   PIR; S71618; S71618.
DR   RefSeq; NP_001452.1; -.
DR   UniGene; Hs.642706; -.
DR   PhosphoSite; P49326; -.
DR   PRIDE; P49326; -.
DR   Ensembl; ENSG00000131781; Homo sapiens.
DR   GeneID; 2330; -.
DR   KEGG; hsa:2330; -.
DR   GeneCards; GC01M145124; -.
DR   HGNC; HGNC:3773; FMO5.
DR   HPA; HPA012373; -.
DR   MIM; 603957; gene.
DR   PharmGKB; PA28189; -.
DR   HOGENOM; P49326; -.
DR   HOVERGEN; P49326; -.
DR   NextBio; 9457; -.
DR   ArrayExpress; P49326; -.
DR   CleanEx; HS_FMO5; -.
DR   GermOnline; ENSG00000131781; Homo sapiens.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031227; C:intrinsic to endoplasmic reticulum membrane; IEA:InterPro.
DR   GO; GO:0005792; C:microsome; NAS:UniProtKB.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0004499; F:flavin-containing monooxygenase activity; NAS:UniProtKB.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012143; dManiline_mOase.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR000960; Flavin_mOase.
DR   InterPro; IPR002257; Flavin_mOase_5.
DR   Pfam; PF00743; FMO-like; 1.
DR   PIRSF; PIRSF000332; FMO; 1.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00370; FMOXYGENASE.
DR   PRINTS; PR01125; FMOXYGENASE5.
DR   ProDom; PD000139; FAD_pyr_redox; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Endoplasmic reticulum; FAD; Flavoprotein; Membrane;
KW   Methylation; Microsome; Monooxygenase; NADP; Oxidoreductase;
KW   Polymorphism; Transmembrane.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    533       Dimethylaniline monooxygenase [N-oxide-
FT                                forming] 5.
FT                                /FTId=PRO_0000147665.
FT   NP_BIND      10     15       FAD (Potential).
FT   NP_BIND     192    197       NADP (By similarity).
FT   MOD_RES       2      2       N-acetylthreonine (By similarity).
FT   MOD_RES       5      5       Omega-N-methylated arginine (By
FT                                similarity).
FT   VARIANT     400    400       P -> A.
FT                                /FTId=VAR_022308.
FT   VARIANT     457    457       P -> L.
FT                                /FTId=VAR_015370.
FT   VARIANT     506    506       R -> S.
FT                                /FTId=VAR_022309.
FT   CONFLICT    351    351       S -> P (in Ref. 2; CAA87633).
SQ   SEQUENCE   533 AA;  60221 MW;  F9D9F092F1DD71DA CRC64;
     MTKKRIAVIG GGVSGLSSIK CCVEEGLEPV CFERTDDIGG LWRFQENPEE GRASIYKSVI
     INTSKEMMCF SDYPIPDHYP NFMHNAQVLE YFRMYAKEFD LLKYIRFKTT VCSVKKQPDF
     ATSGQWEVVT ESEGKKEMNV FDGVMVCTGH HTNAHLPLES FPGIEKFKGQ YFHSRDYKNP
     EGFTGKRVII IGIGNSGGDL AVEISQTAKQ VFLSTRRGAW ILNRVGDYGY PADVLFSSRL
     THFIWKICGQ SLANKYLEKK INQRFDHEMF GLKPKHRALS QHPTLNDDLP NRIISGLVKV
     KGNVKEFTET AAIFEDGSRE DDIDAVIFAT GYSFDFPFLE DSVKVVKNKI SLYKKVFPPN
     LERPTLAIIG LIQPLGAIMP ISELQGRWAT QVFKGLKTLP SQSEMMAEIS KAQEEIDKRY
     VESQRHTIQG DYIDTMEELA DLVGVRPNLL SLAFTDPKLA LHLLLGPCTP IHYRVQGPGK
     WDGARKAILT TDDRIRKPLM TRVVERSSSM TSTMTIGKFM LALAFFAIII AYF
//