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UniProtKB/Swiss-Prot entry P47985


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UCRI_HUMAN
Primary accession number P47985
Secondary accession numbers Q6NVX5 Q9UPH2
Integrated into Swiss-Prot on February 1, 1996
Sequence was last modified on April 3, 2007 (Sequence version 2)
Annotations were last modified on    September 23, 2008 (Entry version 84)
Name and origin of the protein
Protein name Cytochrome b-c1 complex subunit Rieske, mitochondrial [Precursor]
Synonyms EC 1.10.2.2
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Rieske iron-sulfur protein
RISP
Complex III subunit 5
Contains Cytochrome b-c1 complex subunit 11
     (Ubiquinol-cytochrome c reductase 8 kDa protein)
     (Complex III subunit IX)
Gene name
Name: UQCRFS1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2158323 [NCBI, ExPASy, EBI, Israel, Japan]
Nishikimi M., Hosokawa Y., Toda H., Suzuki H., Ozawa T.;
"The primary structure of human Rieske iron-sulfur protein of mitochondrial cytochrome bc1 complex deduced from cDNA analysis.";
Biochem. Int. 20:155-160(1990).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(94)00683-J; PubMed=7721092 [NCBI, ExPASy, EBI, Israel, Japan]
Pennacchio L., Bergmann A., Fukushima A., Salemi A., Okubo K., Lennon G.;
"Structure, sequence and location of the UQCRFS1 gene for the human Rieske Fe-S protein.";
Gene 155:207-211(1995).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature02399; PubMed=15057824 [NCBI, ExPASy, EBI, Israel, Japan]
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.;
"The DNA sequence and biology of human chromosome 19.";
Nature 428:529-535(2004).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain, Lung, and Lymph;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PARTIAL PROTEIN SEQUENCE OF 79-90.
TISSUE=Kidney;
PubMed=9150947 [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone B., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.;
"Renal cell carcinoma and normal kidney protein expression.";
Electrophoresis 18:599-604(1997).
Comments
  • FUNCTION: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • FUNCTION: The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex (By similarity).
  • CATALYTIC ACTIVITY: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
  • COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
  • SUBUNIT: The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).
  • SUBCELLULAR LOCATION: Mitochondrion inner membrane.
  • MISCELLANEOUS: The Rieske protein is a high potential 2Fe-2S protein.
  • SIMILARITY: Contains 1 Rieske domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L32977; AAC41754.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L32917; AAC41754.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC007786; AAD38242.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC000649; AAH00649.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC010035; AAH10035.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC067832; AAH67832.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_005994.2; -.
UniGene Hs.170107
3D structure databases
HSSP P13272; 1RIE. [HSSP ENTRY / PDB]
SMR P47985; 1-57, 79-274.
ModBase P47985.
PTM databases
PhosphoSite P47985; -.
Enzyme and pathway databases
Reactome REACT_6305; Electron Transport Chain.
2D gel databases
SWISS-2DPAGE P47985; -.
HSC-2DPAGE P47985; -.
OGP P47985; -.
REPRODUCTION-2DPAGE IPI00026964; -.
Organism-specific databases
H-InvDB HIX0014973; -.
HIX0041192; -.
HIX0080950; -.
HGNC HGNC:12587; UQCRFS1.
GenAtlas UQCRFS1.
MIM 191327; gene. [NCBI / EBI]
PharmGKB PA37218; -.
GeneCards P47985.
Gene expression databases
ArrayExpress P47985; -.
CleanEx HS_UQCRFS1; -.
GermOnline ENSG00000169021; Homo sapiens.
Ontologies
GO
GO:0005750; Cellular component: mitochondrial respiratory chain complex III (non-traceable author statement from UniProtKB).
GO:0008121; Molecular function: ubiquinol-cytochrome-c reductase activity (non-traceable author statement from UniProtKB).
GO:0055114; Biological process: oxidation reduction (non-traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR014349; Rieske.
IPR005805; Rieske_C.
IPR006317; Rieske_proteo.
IPR005806; Rieske_reg.
IPR015248; Ubiq_cytc_red_N.
IPR004192; UCR_TM_region.
Graphical view of domain structure.
Gene3D G3DSA:2.102.10.10; Rieske_reg; 1.
PANTHER PTHR10134; Rieske; 1.
Pfam PF00355; Rieske; 1.
PF09165; Ubiq-Cytc-red_N; 1.
PF02921; UCR_TM; 1.
Pfam graphical view of domain structure.
PRINTS PR00162; RIESKE.
TIGRFAMs TIGR01416; Rieske_proteo; 1.
PROSITE PS51296; RIESKE; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P47985.
Genome annotation databases
Ensembl ENSG00000169021; Homo sapiens. [Contig view]
GeneID 7386; -.
KEGG hsa:7386; -.
NMPDR fig|9606.3.peg.16154; -.
Phylogenomic databases
HOGENOM P47985; -.
HOVERGEN P47985; -.
Other
LinkHub P47985; -.
SOURCE UQCRFS1; Homo sapiens.
ProtoNet P47985.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Respiratory chain; Transit peptide; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1    78  78     Cytochrome b-c1 complex subunit 11 (By similarity). PRO_0000307241
TRANSIT   1    78  78     Mitochondrion. 
CHAIN   79   274  196     Cytochrome b-c1 complex subunit Rieske, mitochondrial. PRO_0000030664
DOMAIN   187   272  86     Rieske. 
METAL   217   217        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   219   219        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
METAL   236   236        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   239   239        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
DISULFID   222   238        By similarity. 
CONFLICT   6     6        S -> A (in Ref. 1, 2; AAC41754 and 4; AAH00649/AAH10035/AAH67832). 
CONFLICT   73    73        P -> H (in Ref. 4; AAH67832). 
Sequence information
Length: 274 AA [This is the length of the unprocessed precursor] Molecular weight: 29668 Da [This is the MW of the unprocessed precursor] CRC64: 8DB51634DEB039B0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLSVASRSGP FAPVLSATSR GVAGALRPLV QATVPATPEQ PVLDLKRPFL SRESLSGQAV 

        70         80         90        100        110        120 
RRPLVASVGL NVPASVCYSH TDIKVPDFSE YRRLEVLDST KSSRESSEAR KGFSYLVTGV 

       130        140        150        160        170        180 
TTVGVAYAAK NAVTQFVSSM SASADVLALA KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT 

       190        200        210        220        230        240 
QKEIEQEAAV ELSQLRDPQH DLDRVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG 

       250        260        270 
SHYDASGRIR LGPAPLNLEV PTYEFTSDDM VIVG 

P47985 in FASTA format

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