NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Liver;
DOI=10.1006/abbi.1993.1537; PubMed=8105753 [NCBI, ExPASy, EBI, Israel, Japan]
Crestani M., Galli G., Chiang J.Y.;
"Genomic cloning, sequencing, and analysis of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7).";
Arch. Biochem. Biophys. 306:451-460(1993).

Comments

CATALYTIC ACTIVITY: Cholesterol + NADPH + O₂ = 7-alpha-hydroxycholesterol + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Lipid metabolism; bile acid biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L04690; AAA03751.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S39399; S39399.

3D structure databases

ModBase

P46634.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

P46634.

Phylogenomic databases

HOVERGEN

P46634; -.

Other

ProtoNet

P46634.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol metabolism; Endoplasmic reticulum; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Steroid metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 504`	`504`	`Cytochrome P450 7A1.`	`PRO_0000051900`
`METAL`	`444 444`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 504 AA [This is the length of the unprocessed precursor]

Molecular weight: 57447 Da [This is the MW of the unprocessed precursor]

CRC64: BD5E521D0C195257 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMTISLIWGI AMVVCCCIWV IFDRRRRKAG EPPLENGLIP YLGCALKFGS NPLEFLRANQ 

        70         80         90        100        110        120 
RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPNDGN 

       130        140        150        160        170        180 
TTENINNTFT KTLQGDALHS LSEAMMQNLQ FVLRPPDLPK SKSDAWVTEG MYAFCYRVMF 

       190        200        210        220        230        240 
EAGYLTLFGR DTSKPDTQRV LILNNLNSFK QFDQVFPALV AGLPIHLFKA AHKAREQLAE 

       250        260        270        280        290        300 
GLKHENLSVR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL AILWASQANT IPATFWSLFQ 

       310        320        330        340        350        360 
MIRSPDALRA ASEEVNGALQ SAGQKLSSEG NAIYLDQIQL NNLPVLDSII KEALRLSSAS 

       370        380        390        400        410        420 
LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPAI YPDPLTFKYD RYLDENKKAK 

       430        440        450        460        470        480 
TSFYSNGNKL KYFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSYFELELV ESHVKCPPLD 

       490        500 
QSRAGLGILP PLNDIEFKYK LKHL

P46634 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools