[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=DSM 405 / IFO 15313 / YP-T; PubMed=7516791 [NCBI, ExPASy, EBI, Israel, Japan] Ghim S.Y., Nielsen P., Neuhard J.; "Molecular characterization of pyrimidine biosynthesis genes from the thermophile Bacillus caldolyticus."; Microbiology 140:479-491(1994).

Comments

CATALYTIC ACTIVITY: (S)-dihydroorotate + O₂ = orotate + H₂O₂.
COFACTOR: Binds 1 FMN per subunit (By similarity).
PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6 .
SUBUNIT: Heterotetramer of 2 pyrK and 2 pyrD subunits (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Copyright

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Cross-references

Sequence databases

EMBL

X73308; CAA51741.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I40171; I40171.

3D structure databases

HSSP

P54322; 1EP2. [HSSP ENTRY / PDB]

ModBase

P46539.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0004158;	Molecular function: dihydroorotate oxidase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00224; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR012135; DHO_DHase_1_2.
IPR005720; DHO_DHase_1_core.
IPR001295; Dihydroorotate_DHase_core.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF01180; DHO_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000164; DHO_oxidase; 1.

TIGRFAMs

TIGR01037; pyrD_sub1_fam; 1.

PROSITE

PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Flavoprotein; FMN; Oxidoreductase; Pyrimidine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 313`	`313`	`Dihydroorotate dehydrogenase, catalytic subunit.`	`PRO_0000148387`
`ACT_SITE`	`130 130`		`Nucleophile (By similarity).`

Sequence information

Length: 313 AA [This is the length of the unprocessed precursor]

Molecular weight: 32857 Da [This is the MW of the unprocessed precursor]

CRC64: 8EB76F840A49798E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNRLAVELPG LSLKNPIMPA SGCFGFGREY ARFYDLSVLG AIMIKATTKE PRFGNPTPRV 

        70         80         90        100        110        120 
AETPGGMLNA IGLQNPGLDK VLEEELPWLE QFDVPIIANI AGSTVEEYVE VAEAISKAPN 

       130        140        150        160        170        180 
VHALELNISC PNVKKGGIAF GTVPDVAAEL TRLVKEVAAV PVYVNVSPNV TDIVAMAKAI 

       190        200        210        220        230        240 
EQAGADGLTM INTLVGMRID VKTGRPILAN GTGGLSGPAV KPIAIRMIYE VSQAVSIPII 

       250        260        270        280        290        300 
GMGGIQTAED VLEFFYAGAS AVAVGTANFV DPFVCPTIIA DLPALLDDLG IGHISECIGR 

       310 
SWKTGAHAVH CRA

P46539 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools