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UniProtKB/Swiss-Prot entry P45868


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAO2_BACSU
Primary accession number P45868
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on November 1, 1995 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 68)
Name and origin of the protein
Protein name Probable NAD-dependent malic enzyme 2
Synonyms NAD-ME 2
EC 1.1.1.38
Gene name
Name: maeA
OrderedLocusNames: BSU37050
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=9353933 [NCBI, ExPASy, EBI, Israel, Japan]
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.;
"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).";
Microbiology 143:3313-3328(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[3]
FUNCTION, CHARACTERIZATION, AND REGULATION.
DOI=10.1099/mic.0.26256-0; PubMed=12949160 [NCBI, ExPASy, EBI, Israel, Japan]
Doan T., Servant P., Tojo S., Yamaguchi H., Lerondel G., Yoshida K., Fujita Y., Aymerich S.;
"The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.";
Microbiology 149:2331-2343(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z49782; CAA89880.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99122; CAB15722.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S55433; S55433.
RefSeq NP_391586.1; -.
3D structure databases
HSSP P27443; 1LLQ. [HSSP ENTRY / PDB]
ModBase P45868.
Enzyme and pathway databases
BioCyc BSUB224308:BSU3703-MON; -.
Organism-specific databases
SubtiList BG11312; maeA. [Micado]
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS P45868.
Genome annotation databases
GeneID 937045; -.
GenomeReviews AL009126_GR; BSU37050.
KEGG bsu:BSU37050; -.
NMPDR fig|224308.1.peg.3712; -.
Phylogenomic databases
HOGENOM P45868; -.
Genome annotation databases
CMR P45868; BSU37050.
Other
ProtoNet P45868.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   582  582     Probable NAD-dependent malic enzyme 2. PRO_0000160209
ACT_SITE   122   122        Proton donor (By similarity). 
ACT_SITE   195   195        Proton acceptor (By similarity). 
METAL   266   266        Divalent metal cation (By similarity). 
METAL   267   267        Divalent metal cation (By similarity). 
METAL   290   290        Divalent metal cation (By similarity). 
BINDING   290   290        NAD (By similarity). 
BINDING   439   439        NAD (By similarity). 
SITE   290   290  1     Important for activity (By similarity). 
Sequence information
Length: 582 AA [This is the length of the unprocessed precursor] Molecular weight: 64103 Da [This is the MW of the unprocessed precursor] CRC64: E02821E341C20302 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGYYLTWLTI SRKKEICLNN IKKTKEGHLE TTLRGKEVLS IPTLNKGVAF SLEERQELGL 

        70         80         90        100        110        120 
EGLLPPTVLS LDQQAQRAYE QFQAQPDRLR QNVYLSDLAN RNEVLFYKLL KNHLREMLPV 

       130        140        150        160        170        180 
VYTPTVGEAI QEYSHEYRRP QGIYLSIDNI DGIEKAFENL HATAGDIDLI VATDSESILG 

       190        200        210        220        230        240 
IGDWGVGGIN IAIGKLAVYT AAAGIDPSRV IPVVLDVGTN NEKLLNDPLY IGNKHERVQG 

       250        260        270        280        290        300 
ERYEAFIDAY VKAALKFFPK ALLHWEDLGN KNARNIMKKY NHEILTFNDD IQGTGAITLA 

       310        320        330        340        350        360 
GVLAAMKKTG ASIKDQRVVI FGAGSAGIGI ADQIRDTMVL AGLSEEEANK RFYTLDYRGL 

       370        380        390        400        410        420 
LTEDIEGILD FQKPYLRNAD EVKDWKRDEK GQIPFDEVVR QAKPTILIGT SGVSGAFTEE 

       430        440        450        460        470        480 
IVKEMASHVD RPVIMPMSNP THLAEAVPED LFKWTDGKVL IATGSPFDNV EYNGVSYEIG 

       490        500        510        520        530        540 
QSNNAFAFPG LGLGSIVAEA RIITPAMFAA TADAIAEMVD LETPGAGLLP SIDKLQEVSI 

       550        560        570        580 
QVAIAVAEAA IKDGVANRQP EDVKQAVLDA MWTPEYKKVI AK 

P45868 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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