ID   NIR_LEPMC               Reviewed;         185 AA.
AC   P43504;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-NOV-2008, entry version 50.
DE   RecName: Full=Nitrite reductase [NAD(P)H];
DE            EC=1.7.1.4;
DE   Flags: Fragment;
GN   Name=NIIA;
OS   Leptosphaeria maculans (Blackleg fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Dothideomycetes; Pleosporomycetidae; Pleosporales; Leptosphaeriaceae;
OC   Leptosphaeria; Leptosphaeria maculans complex.
OX   NCBI_TaxID=5022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Isolate 19;
RX   MEDLINE=95309720; PubMed=7789806; DOI=10.1016/0378-1119(95)00169-7;
RA   Williams R.S.B., Davis M.A., Howlett B.J.;
RT   "The nitrate and nitrite reductase-encoding genes of Leptosphaeria
RT   maculans are closely linked and transcribed in the same direction.";
RL   Gene 158:153-154(1995).
CC   -!- CATALYTIC ACTIVITY: Ammonium hydroxide + 3 NAD(P)(+) + H(2)O =
CC       nitrite + 3 NAD(P)H.
CC   -!- COFACTOR: Binds 1 siroheme per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; U18793; AAA82739.1; -; Genomic_DNA.
DR   PIR; PC4032; PC4032.
DR   HSSP; Q52437; 1D7Y.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   InterPro; IPR001327; Pyr_OxRdtase_NAD_bd.
DR   Pfam; PF00070; Pyr_redox; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PROSITE; PS00365; NIR_SIR; PARTIAL.
PE   3: Inferred from homology;
KW   2Fe-2S; FAD; Flavoprotein; Heme; Iron; Iron-sulfur; Metal-binding;
KW   NADP; Nitrate assimilation; Oxidoreductase.
FT   CHAIN         1   >185       Nitrite reductase [NAD(P)H].
FT                                /FTId=PRO_0000199959.
FT   NP_BIND      72    107       FAD (Potential).
FT   NON_TER     185    185
SQ   SEQUENCE   185 AA;  20756 MW;  19D67F97D386E7F9 CRC64;
     MAATLPLLTQ GIEPVSGESY SPPGERHVQA TWGSKDGNIS NTEWNDPEAN RLPEKVAELE
     KKGELNNSHP RRRVVVVGLG MVGVAFIEKL MKYDIKRREY DIIVIGEEPH LAYNRVGLTS
     FFQHRQVENL YLNPQEWYSS MPEDSLHYHL NTLVTEIDSE NKTVKTSSGQ AVSYDILVLA
     TGSSS
//