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UniProtKB/Swiss-Prot entry P42770


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GSHRP_ARATH
Primary accession number P42770
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on November 1, 1995 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 71)
Name and origin of the protein
Protein name Glutathione reductase, chloroplastic [Precursor]
Synonyms GRase
GR
EC 1.8.1.7
Protein EMBRYO DEFECTIVE 2360
Gene name
Name: EMB2360
OrderedLocusNames: At3g54660
ORFNames: T5N23_20
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE.
STRAIN=cv. Columbia;
Kubo A., Sano T., Saji H., Tanaka K., Kondo N., Tanaka K.;
"Primary structure and properties of glutathione reductase from Arabidopsis thaliana.";
Plant Cell Physiol. 34:1259-1266(1993).
[2]
NUCLEOTIDE SEQUENCE.
STRAIN=cv. Columbia;
Kubo A.;
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048706; PubMed=11130713 [NCBI, ExPASy, EBI, Israel, Japan]
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
Nature 408:820-822(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D14049; BAA03137.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D89620; BAA19653.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL138650; CAB77586.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T47625; T47625.
RefSeq NP_191026.1; -.
UniGene At.21776
3D structure databases
HSSP P06715; 1GER. [HSSP ENTRY / PDB]
ModBase P42770.
Organism-specific databases
GeneFarm 2285; 251.
TAIR At3g54660; -.
Gene expression databases
ArrayExpress P42770; -.
GermOnline AT3G54660; Arabidopsis thaliana.
Family and domain databases
InterPro IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR006324; Glut_reduct_pln.
IPR000815; Hg_reductase.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
IPR012999; Pyr_OxRdtase_I_AS.
IPR001327; Pyr_OxRdtase_NAD_bd.
Graphical view of domain structure.
Gene3D G3DSA:3.30.390.30; Pyr_redox_dim; 1.
Pfam PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
PF02852; Pyr_redox_dim; 1.
Pfam graphical view of domain structure.
PRINTS PR00368; FADPNR.
PR00945; HGRDTASE.
PR00411; PNDRDTASEI.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01424; gluta_reduc_2; 1.
PROSITE PS00076; PYRIDINE_REDOX_1; 1.
BLOCKS P42770.
Genome annotation databases
GeneID 824631; -.
GenomeReviews BA000014_GR; AT3G54660.
KEGG ath:AT3G54660; -.
Other
ProtoNet P42770.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chloroplast; Complete proteome; FAD; Flavoprotein; NADP; Oxidoreductase; Plastid; Redox-active center; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    74  74     Chloroplast (Potential). 
CHAIN   75   565  491     Glutathione reductase, chloroplastic. PRO_0000030280
NP_BIND   126   135  10     FAD (By similarity). 
ACT_SITE   529   529        Proton acceptor (By similarity). 
BINDING   291   291        NADP (By similarity). 
BINDING   297   297        NADP (By similarity). 
DISULFID   135   140        Redox-active (By similarity). 
Sequence information
Length: 565 AA [This is the length of the unprocessed precursor] Molecular weight: 60852 Da [This is the MW of the unprocessed precursor] CRC64: 29C2CD0E68ED4E4A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MASTPKLTST ISSSSPSLQF LCKKLPIAIH LPSSSSSSFL SLPKTLTSLY SLRPRIALLS 

        70         80         90        100        110        120 
NHRYYHSRRF SVCASTDNGA ESDRHYDFDL FTIGAGSGGV RASRFATSFG ASAAVCELPF 

       130        140        150        160        170        180 
STISSDTAGG VGGTCVLRGC VPKKLLVYAS KYSHEFEDSH GFGWKYETEP SHDWTTLIAN 

       190        200        210        220        230        240 
KNAELQRLTG IYKNILSKAN VKLIEGRGKV IDPHTVDVDG KIYTTRNILI AVGGRPFIPD 

       250        260        270        280        290        300 
IPGKEFAIDS DAALDLPSKP KKIAIVGGGY IALEFAGIFN GLNCEVHVFI RQKKVLRGFD 

       310        320        330        340        350        360 
EDVRDFVGEQ MSLRGIEFHT EESPEAIIKA GDGSFSLKTS KGTVEGFSHV MFATGRKPNT 

       370        380        390        400        410        420 
KNLGLENVGV KMAKNGAIEV DEYSQTSVPS IWAVGDVTDR INLTPVALME GGALAKTLFQ 

       430        440        450        460        470        480 
NEPTKPDYRA VPCAVFSQPP IGTVGLTEEQ AIEQYGDVDV YTSNFRPLKA TLSGLPDRVF 

       490        500        510        520        530        540 
MKLIVCANTN KVLGVHMCGE DSPEIIQGFG VAVKAGLTKA DFDATVGVHP TAAEEFVTMR 

       550        560 
APTRKFRKDS SEGKASPEAK TAAGV 

P42770 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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