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UniProtKB/Swiss-Prot entry P42435


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NASD_BACSU
Primary accession number P42435
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1995
Sequence was last modified on November 1, 1995 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 81)
Name and origin of the protein
Protein name Nitrite reductase [NAD(P)H]
Synonym EC 1.7.1.4
Gene name
Name: nasD
Synonyms: nasBC, nirB
OrderedLocusNames: BSU03300
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=7868621 [NCBI, ExPASy, EBI, Israel, Japan]
Ogawa K., Akagawa E., Yamane K., Sun Z.-W., Lacelle M., Zuber P., Nakano M.M.;
"The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.";
J. Bacteriol. 177:1409-1413(1995).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=8969502 [NCBI, ExPASy, EBI, Israel, Japan]
Yamane K., Kumano M., Kurita K.;
"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes.";
Microbiology 142:3047-3056(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[4]
REGULATION BY TNRA.
STRAIN=168;
DOI=10.1006/jmbi.2000.3846; PubMed=10864496 [NCBI, ExPASy, EBI, Israel, Japan]
Wray L.V. Jr., Zalieckas J.M., Fisher S.H.;
"Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor.";
J. Mol. Biol. 300:29-40(2000).
[5]
REGULATION.
STRAIN=168;
DOI=10.1128/JB.183.23.6815-6821.2001; PubMed=11698370 [NCBI, ExPASy, EBI, Israel, Japan]
Marino M., Cruz Ramos H., Hoffmann T., Glaser P., Jahn D.;
"Modulation of anaerobic energy metabolism of Bacillus subtilis by arfM (ywiD).";
J. Bacteriol. 183:6815-6821(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D30689; BAA06354.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D50453; BAA08964.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99105; CAB12124.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR I40029; I40029.
RefSeq NP_388212.1; -.
3D structure databases
ModBase P42435.
Enzyme and pathway databases
BioCyc BSUB224308:BSU0331-MON; -.
Organism-specific databases
SubtiList BG11096; nasD. [Micado]
Family and domain databases
InterPro IPR007419; BFD_Fer2_bd.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
IPR012744; Nitri_red_NirB.
IPR017121; Nitrite_Rdtase_lsu.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR001327; Pyr_OxRdtase_NAD_bd.
Graphical view of domain structure.
Pfam PF04324; Fer2_BFD; 2.
PF01077; NIR_SIR; 1.
PF03460; NIR_SIR_ferr; 1.
PF00070; Pyr_redox; 2.
PF07992; Pyr_redox_2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037149; NirB; 1.
PRINTS PR00368; FADPNR.
PR00411; PNDRDTASEI.
PR00397; SIROHAEM.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR02374; nitri_red_nirB; 1.
PROSITE PS00365; NIR_SIR; FALSE_NEG.
BLOCKS P42435.
Genome annotation databases
GeneID 938329; -.
GenomeReviews AL009126_GR; BSU03300.
KEGG bsu:BSU03300; -.
NMPDR fig|224308.1.peg.331; -.
Phylogenomic databases
HOGENOM P42435; -.
Other
LinkHub P42435; -.
Genome annotation databases
CMR P42435; BSU03300.
Other
ProtoNet P42435.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
4Fe-4S; Complete proteome; FAD; Flavoprotein; Heme; Iron; Iron-sulfur; Metal-binding; NADP; Nitrate assimilation; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   805  805     Nitrite reductase [NAD(P)H]. PRO_0000199961
NP_BIND   43    79  37     FAD (Potential). 
NP_BIND   193   223  31     NAD or NADP (Potential). 
METAL   635   635        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   641   641        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   675   675        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   679   679        Iron (siroheme axial ligand) (By similarity). 
METAL   679   679        Iron-sulfur (4Fe-4S) (By similarity). 
Sequence information
Length: 805 AA [This is the length of the unprocessed precursor] Molecular weight: 88432 Da [This is the MW of the unprocessed precursor] CRC64: 5ED3295AF3E0CA29 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGKKQLVLVG NGMAGVRAIE EILSVAKDEF QITIFGAEPH PNYNRILLSK VLQGDTDIKD 

        70         80         90        100        110        120 
ITLNDWDWYE ENNIQLYTNE TVIKVDTENK TVITDADRIQ PYDELILATG SVPFILPIPG 

       130        140        150        160        170        180 
ADKKGVTAFR DIKDTDTMLA ASKQYKKAAV IGGGLLGLEA ARGLLNLGMD VSVIHLAPFL 

       190        200        210        220        230        240 
MERQLDATAG RLLQNELEKQ GMTFLLEKQT EEIVGDDRVE GLRFKDGTSI EADLVVMAVG 

       250        260        270        280        290        300 
IRPNTTLGAE SGIPVNRGII VNDYMQTEIP HIYAVGECAE HRGIAYGLVA PLYEQAKVLA 

       310        320        330        340        350        360 
KHMCGIETKP YEGSVLSTQL KVSGVEVFSA GDFNESEEKK AIKVFDEQDG IYKKIVLRGN 

       370        380        390        400        410        420 
QIVGAVLFGD SSEGNRLFSM IQKEADISET SKISILQPLS QEAGTSITAA MSDDEIICGC 

       430        440        450        460        470        480 
NGVSKGAIIQ AIQEKGCSST DEIKACTGAS RSCGGCKPLV EEILQHTLGS DFDASAQKEA 

       490        500        510        520        530        540 
ICGCTTLSRD EVVEEIKAKG LSHTREVMNV LGWKTPEGCS KCRPALNYYL GMINPTKYED 

       550        560        570        580        590        600 
DRTSRFVNER MHANIQKDGT YSVVPRMYGG VTNSTDLRKI ADVVDKYEIP LVKMTGGQRI 

       610        620        630        640        650        660 
DLIGVKKEDL PKVWEDLDMP SGYAYGKTLR TVKTCVGEQF CRFGTQDSMA LGIALEKKFE 

       670        680        690        700        710        720 
GLNTPHKVKM AVSACPRNCA ESGIKDLGVV GIDGGWELYV GGNGGTHLRA GDLLMKVKTN 

       730        740        750        760        770        780 
EEVLEYAGAY LQYYRETANY LERTSAWLER VGLSHVQSVL NDPEKRQELN GRMNETLSVH 

       790        800 
KDPWKDFLED KQTSKELFEN VVTTS 

P42435 in FASTA format

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