NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=SIC1;
Imanaka T., Ohta T., Sakoda H., Widhyastuti N., Matsuoka M.;
"Cloning, nucleotide sequence, and efficient expression of the gene coding for thermostable aldehyde dehydrogenase from Bacillus stearothermophilus, and characterization of the enzyme.";
J. Ferment. Bioeng. 76:161-167(1993).

Comments

FUNCTION: Oxidizes several aliphatic aldehydes, particularly c6-aliphatic aldehyde and hexanal, but does not oxidize benzaldehyde.
CATALYTIC ACTIVITY: An aldehyde + NAD(P)⁺ + H₂O = an acid + NAD(P)H.
BIOPHYSICOCHEMICAL PROPERTIES:

Temperature dependence: Optimum temperature is 55-60 degrees Celsius;
PATHWAY: Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2 .
SUBUNIT: Homotetramer.
SIMILARITY: Belongs to the aldehyde dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D13846; BAA02975.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I39769; I39769.

3D structure databases

HSSP

P51977; 1BXS. [HSSP ENTRY / PDB]

ModBase

P42329.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11699; Aldehyde_dehyd; 1.

Pfam

PF00171; Aldedh; 1.
Pfam graphical view of domain structure.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; 1.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 488`	`488`	`Aldehyde dehydrogenase, thermostable.`	`PRO_0000056442`
`NP_BIND`	`233 238`	`6`	`NAD (By similarity).`
`ACT_SITE`	`255 255`		`By similarity.`
`ACT_SITE`	`289 289`		`By similarity.`

Sequence information

Length: 488 AA [This is the length of the unprocessed precursor]

Molecular weight: 52915 Da [This is the MW of the unprocessed precursor]

CRC64: 29E824451985D9ED [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKVQTEIKTY FNYINGNWVS SVSNNVEPSI NPANRHDIVG YVQRSTLEDV NEAVTAANEA 

        70         80         90        100        110        120 
QTSWWKRSGV ERGEYLYKAA HILEQCLQDI AETMTREMGK TLAEAKAETM RGVHILRYYA 

       130        140        150        160        170        180 
GEGARKIGDV IPSSDSEGLL FTTRVPLGVV GVISPWNFPV AIPIWKMAPA LVYGNTVVLK 

       190        200        210        220        230        240 
PASETAVTAA KVIECFHEAG FPKGVVNMVC GSGSVVGQGI ANHPDIDGVT FTGSNTVGKQ 

       250        260        270        280        290        300 
VGRAAFERGA KYQLEMGGKN PVIVAKDADL DLAVEGTISG GLRSTGQKCT ATSRVFIERE 

       310        320        330        340        350        360 
VYEPFKAKLL ERVKQLKIGN GLDAETWMGP CASESQFHTV LSYIEKGKSE GAKLIYGGNR 

       370        380        390        400        410        420 
CLEGELANGF FVEPTIFEDV DLQMTIAREE IFGPVLALIQ VDSIEEAIKL ANDTEYGLSA 

       430        440        450        460        470        480 
SIYTKNIGNA LEFIKDIEAG LIKVNAETAG VEFQAPFGGM KQSSSHSREQ GQAAIEFFTS 


IKTVFVKA

P42329 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools