NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1007/s002530050606; PubMed=8703431 [NCBI, ExPASy, EBI, Israel, Japan]
Coque J., Enguita F.J., Cardoza R.E., Martin J.F., Liras P.;
"Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase.";
Appl. Microbiol. Biotechnol. 44:605-609(1996).

Comments

FUNCTION: Hydroxylation of desacetoxicephalosporin C in 3'position to form deacetylcephalosporin C.
CATALYTIC ACTIVITY: Deacetoxycephalosporin C + 2-oxoglutarate + O₂ = deacetylcephalosporin C + succinate + CO₂.
COFACTOR: Iron.
PATHWAY: Antibiotic biosynthesis; cephalosporin C biosynthesis .
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z21687; CAA79803.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S40253; S40253.

3D structure databases

HSSP

P18548; 1RXF. [HSSP ENTRY / PDB]

ModBase

P42219.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Antibiotic biosynthesis; Iron; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 310`	`310`		`Deacetoxycephalosporin C hydroxylase.`	`PRO_0000219512`

Sequence information

Length: 310 AA [This is the length of the unprocessed precursor]

Molecular weight: 34364 Da [This is the MW of the unprocessed precursor]

CRC64: 9983EF1AC1B416E6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSDKTVPVFS MAELRDGSRQ DEFREWARRG VFYLTGYGAT ERDHRVATDT AMDFFAQGTA 

        70         80         90        100        110        120 
EEKQAVTTKV PTMRRGYSAL EAESTAQVTN TGTYTDYSMS YSMGIGGNLF PSKEFESVWT 

       130        140        150        160        170        180 
DYFDSLYRAA QETARLVLTA AGTYDGEDLD TLLDCDPVLR LRYFPEVPEH RAAEYEPRRM 

       190        200        210        220        230        240 
APHYDLSIIT FIHQTPCANG FVSLQAEVDG EMVSLPHVED AVVVLCGAIA PLVTQGAVPA 

       250        260        270        280        290        300 
PNHHVVSPDA SMLKGSDRTS SVFFLRPSTD FTFSVPDARK YGLDVSLDME KATFGDWIGT 

       310 
NYVTMHAVTS

P42219 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools