[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=8226630 [NCBI, ExPASy, EBI, Israel, Japan] Ishii A., Suzuki M., Sahara T., Takada Y., Sasaki S., Fukunaga N.; "Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium, Vibrio sp. strain ABE-1."; J. Bacteriol. 175:6873-6880(1993).
[2]	PROTEIN SEQUENCE OF 2-39. PubMed=1295895 [NCBI, ExPASy, EBI, Israel, Japan] Fukunaga N., Imagawa S., Sahara T., Ishii A., Suzuki M.; "Purification and characterization of monomeric isocitrate dehydrogenase with NADP(+)-specificity from Vibrio parahaemolyticus Y-4."; J. Biochem. 112:849-855(1992).

Comments

CATALYTIC ACTIVITY: Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
CATALYTIC ACTIVITY: Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
SUBUNIT: Monomer (By similarity).
SIMILARITY: Belongs to the monomeric-type IDH family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D14047; BAA03134.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A49341; A49341.

3D structure databases

HSSP

P16100; 1ITW. [HSSP ENTRY / PDB]

ModBase

P41561.

Family and domain databases

InterPro

IPR004436; IsoCit_DHase_NAD_mono.
Graphical view of domain structure.

Pfam

PF03971; IDH; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF009407; IDH_monmr; 1.

TIGRFAMs

TIGR00178; monomer_idh; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Glyoxylate bypass; Magnesium; Metal-binding; NADP; Oxidoreductase; Tricarboxylic acid cycle.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 742`	`741`	`Isocitrate dehydrogenase [NADP] 2.`	`PRO_0000083596`
`NP_BIND`	`84 89`	`6`	`NADP (By similarity).`
`NP_BIND`	`586 587`	`2`	`NADP (By similarity).`
`NP_BIND`	`602 604`	`3`	`NADP (By similarity).`
`REGION`	`134 141`	`8`	`Substrate binding (By similarity).`
`METAL`	`352 352`		`Magnesium or manganese (By similarity).`
`METAL`	`550 550`		`Magnesium or manganese (By similarity).`
`BINDING`	`137 137`		`NADP (By similarity).`
`BINDING`	`147 147`		`Substrate (By similarity).`
`BINDING`	`549 549`		`Substrate (By similarity).`
`BINDING`	`591 591`		`NADP (By similarity).`
`BINDING`	`651 651`		`NADP (By similarity).`
`SITE`	`257 257`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`422 422`	`1`	`Critical for catalysis (By similarity).`

Sequence information

Length: 742 AA [This is the length of the unprocessed precursor]

Molecular weight: 80626 Da [This is the MW of the unprocessed precursor]

CRC64: 63FC256EF2898583 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTDNSKIIY TITDEAPALA TYSLLPIIQA YTASSGINVE TRDISLAGRI LANFPKYLTK 

        70         80         90        100        110        120 
EQRIDDALAE LGELAQTPEA NIIKLPNISA SIPQLEAVIK ELQAKGYDLP HYPAEPQNEA 

       130        140        150        160        170        180 
EESIKLTYAK ILGSAVNPVL REGNSDRRAP ASVKQYARNN PHSMGAWSKE SKSHVAHMAS 

       190        200        210        220        230        240 
GDFYGSEKSV TIDGATSVNI EFVAKNGDVT LLKSKLPLLD KEIIDASVMS KSALVEFFET 

       250        260        270        280        290        300 
EINKAKEEDV LLSLHLKATM MKVSDPVMFG HAVRVFYKDV FAKHAATFEQ LGVDADNGIG 

       310        320        330        340        350        360 
DVYAKIARLP AAQKEEIEAD LQAVYATRPE MAMVDSDKGI TNLHVPSDVI IDASMPAALR 

       370        380        390        400        410        420 
ASGMMWGPDG KQKDTKFMIP DRNYAGVFSA VVDFCRENGA FNPATMGTVP NVGLMAQKAE 

       430        440        450        460        470        480 
EYGSHDKTFT MKAAGTVRVV NSQGERLIEQ EVAQGDIYRM CQVKDAPIQD WVKLAVTRAR 

       490        500        510        520        530        540 
ATGTPTVFWL DENRGHDEQM IKKVNTYLAD HDTTGLDIQI LEPVKACEFT LARVAKGEDA 

       550        560        570        580        590        600 
ISVTGNVLRD YLTDLFPILE LGTSAKMLSI VPLMNGGGLF ETGAGGSAPK HVQQFEKENH 

       610        620        630        640        650        660 
LRWDSLGEFL ALAASLEHVA VTTGNARAQI LADTLDAATG KFLDTNKSPS RKVGELDNRV 

       670        680        690        700        710        720 
VTSILQCIGR NVAAQTTDTE LQASFSSVAQ ALTKQEEKIV AELNAAQGPA IDLNGYYFAD 

       730        740 
TKLAEKAMRP SETFNTILSA LL

P41561 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools