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UniProtKB/Swiss-Prot entry P39976


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DLD3_YEAST
Primary accession number P39976
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on February 1, 1995 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 63)
Name and origin of the protein
Protein name D-lactate dehydrogenase [cytochrome] 3
Synonyms EC 1.1.2.4
D-lactate ferricytochrome C oxidoreductase
D-LCR
Gene name
Name: DLD3
OrderedLocusNames: YEL071W
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169868 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
Nature 387:78-81(1997).
[2]
FUNCTION, AND SUBCELLULAR LOCATION.
DOI=10.1002/(SICI)1097-0061(19990930)15:13<1377::AID-YEA473>3.0.CO;2-0; PubMed=10509019 [NCBI, ExPASy, EBI, Israel, Japan]
Chelstowska A., Liu Z., Jia Y., Amberg D., Butow R.A.;
"Signalling between mitochondria and the nucleus regulates the expression of a new D-lactate dehydrogenase activity in yeast.";
Yeast 15:1377-1391(1999).
[3]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[4]
UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-17, AND MASS SPECTROMETRY.
DOI=10.1038/nbt849; PubMed=12872131 [NCBI, ExPASy, EBI, Israel, Japan]
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.;
"A proteomics approach to understanding protein ubiquitination.";
Nat. Biotechnol. 21:921-926(2003).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-470, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U18795; AAB65016.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S50518; S50518.
RefSeq NP_010843.1; -.
3D structure databases
ModBase P39976.
Protein-protein interaction databases
DIP DIP:6418N; -.
IntAct P39976; -.
Organism-specific databases
CYGD YEL071w; -.
SGD S000000797; DLD3.
Yeast-GFP YEL071W.
Gene expression databases
ArrayExpress P39976; -.
GermOnline YEL071W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0005625; Cellular component: soluble fraction (inferred from direct assay from SGD).
GO:0004458; Molecular function: D-lactate dehydrogenase (cytochrome) activity (inferred from direct assay from SGD).
GO:0030447; Biological process: filamentous growth (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR016167; FAD-bd_2_sub1.
IPR004113; FAD-linked_oxidase_C.
IPR006094; Oxid_FAD_bind_N.
Graphical view of domain structure.
Gene3D G3DSA:3.30.43.10; FAD-binding_2_sub1; 1.
Pfam PF02913; FAD-oxidase_C; 1.
PF01565; FAD_binding_4; 1.
Pfam graphical view of domain structure.
PROSITE PS51387; FAD_PCMH; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P39976.
Proteomic databases
PeptideAtlas P39976; -.
Genome annotation databases
Ensembl YEL071W; Saccharomyces cerevisiae. [Contig view]
GeneID 856638; -.
GenomeReviews U00092_GR; YEL071W.
KEGG sce:YEL071W; -.
NMPDR fig|4932.3.peg.1891; -.
Phylogenomic databases
HOGENOM P39976; -.
Other
LinkHub P39976; -.
ProtoNet P39976.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; FAD; Flavoprotein; Oxidoreductase; Phosphoprotein; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   496  496     D-lactate dehydrogenase [cytochrome] 3. PRO_0000128176
DOMAIN   64   243  180     FAD-binding PCMH-type. 
MOD_RES   470   470        Phosphoserine. 
CROSSLNK   17    17        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin). 
Sequence information
Length: 496 AA [This is the length of the unprocessed precursor] Molecular weight: 55225 Da [This is the MW of the unprocessed precursor] CRC64: 4809F74EDF07F520 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTAAHPVAQL TAEAYPKVKR NPNFKVLDSE DLAYFRSILS NDEILNSQAP EELASFNQDW 

        70         80         90        100        110        120 
MKKYRGQSNL ILLPNSTDKV SKIMKYCNDK KLAVVPQGGN TDLVGASVPV FDEIVLSLRN 

       130        140        150        160        170        180 
MNKVRDFDPV SGTFKCDAGV VMRDAHQFLH DHDHIFPLDL PSRNNCQVGG VVSTNAGGLN 

       190        200        210        220        230        240 
FLRYGSLHGN VLGLEVVLPN GEIISNINAL RKDNTGYDLK QLFIGAEGTI GVVTGVSIVA 

       250        260        270        280        290        300 
AAKPKALNAV FFGIENFDTV QKLFVKAKSE LSEILSAFEF MDRGSIECTI EYLKDLPFPL 

       310        320        330        340        350        360 
ENQHNFYVLI ETSGSNKRHD DEKLTAFLKD TTDSKLISEG MMAKDKADFD RLWTWRKSVP 

       370        380        390        400        410        420 
TACNSYGGMY KYDMSLQLKD LYSVSAAVTE RLNAAGLIGD APKPVVKSCG YGHVGDGNIH 

       430        440        450        460        470        480 
LNIAVREFTK QIEDLLEPFV YEYIASKKGS ISAEHGIGFH KKGKLHYTRS DIEIRFMKDI 

       490 
KNHYDPNGIL NPYKYI 

P39976 in FASTA format

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