ID   DHBA_BACSU              Reviewed;         261 AA.
AC   P39071;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   25-NOV-2008, entry version 68.
DE   RecName: Full=2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase;
DE            EC=1.3.1.28;
DE   AltName: Full=Cold shock protein CSI14;
GN   Name=dhbA; Synonyms=entA; OrderedLocusNames=BSU32000;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg;
RX   MEDLINE=97080557; PubMed=8921902; DOI=10.1016/0378-1119(96)00349-6;
RA   Rowland B.M., Grossman T.H., Osburne M.S., Taber H.W.;
RT   "Sequence and genetic organization of a Bacillus subtilis operon
RT   encoding 2,3-dihydroxybenzoate biosynthetic enzymes.";
RL   Gene 178:119-123(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-256.
RC   STRAIN=168;
RX   MEDLINE=94040785; PubMed=8224884; DOI=10.1016/0378-1119(93)90235-U;
RA   Adams R., Schumann W.;
RT   "Cloning and mapping of the Bacillus subtilis locus homologous to
RT   Escherichia coli ent genes.";
RL   Gene 133:119-121(1993).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-11.
RC   STRAIN=168 / JH642;
RA   Graumann P.L., Schmid R., Marahiel M.A.;
RL   Submitted (OCT-1997) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydro-2,3-dihydroxybenzoate + NAD(+) =
CC       2,3-dihydroxybenzoate + NADH.
CC   -!- PATHWAY: Siderophore biosynthesis; bacillibactin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- INDUCTION: In response to low temperature.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; U26444; AAC44630.1; -; Genomic_DNA.
DR   EMBL; Z99120; CAB15190.1; -; Genomic_DNA.
DR   EMBL; L08644; AAA16899.2; -; Genomic_DNA.
DR   PIR; A69615; A69615.
DR   RefSeq; NP_391080.1; -.
DR   HSSP; P08074; 1CYD.
DR   GeneID; 936579; -.
DR   GenomeReviews; AL009126_GR; BSU32000.
DR   KEGG; bsu:BSU32000; -.
DR   NMPDR; fig|224308.1.peg.3206; -.
DR   SubtiList; BG11019; dhbA.
DR   HOGENOM; P39071; -.
DR   BioCyc; BSUB224308:BSU3197-MON; -.
DR   BioCyc; MetaCyc:MON-13913; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0008667; F:2,3-dihydro-2,3-dihydroxybenzoate dehydroge...; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0009239; P:enterobactin biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006950; P:response to stress; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DHase_sc/Rdtase_SDR.
DR   InterPro; IPR003560; DHB_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR01397; DHBDHDRGNASE.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Direct protein sequencing; NAD;
KW   Oxidoreductase; Stress response.
FT   CHAIN         1    261       2,3-dihydro-2,3-dihydroxybenzoate
FT                                dehydrogenase.
FT                                /FTId=PRO_0000054601.
FT   NP_BIND      12     36       NAD (By similarity).
FT   ACT_SITE    157    157       Proton acceptor (By similarity).
FT   BINDING     144    144       Substrate (By similarity).
FT   CONFLICT    146    146       P -> D (in Ref. 3; AAA16899).
FT   CONFLICT    231    234       IADA -> MRC (in Ref. 3; AAA16899).
FT   CONFLICT    247    256       TMHNLCVDGG -> RCIFMRRCAT (in Ref. 3).
SQ   SEQUENCE   261 AA;  27494 MW;  00B0EFBA53AB407C CRC64;
     MNAKGIEGKI AFITGAAQGI GEAVARTLAS QGAHIAAVDY NPEKLEKVVS SLKAEARHAE
     AFPADVRDSA AIDEITARIE REMGPIDILV NVAGVLRPGL IHSLSDEEWE ATFSVNSTGV
     FNASRSVSKY MMDRRSGSIV TVGSNPAGVP RTSMAAYASS KAAAVMFTKC LGLELAEYNI
     RCNIVSPGST ETDMQWSLWA DENGAEQVIK GSLETFKTGI PLKKLAKPSD IADAVLFLVS
     GQAGHITMHN LCVDGGATLG V
//