NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ILTAT 1.1;
PubMed=1453951 [NCBI, ExPASy, EBI, Israel, Japan]
Aboagye-Kwarteng T., Smith K., Fairlamb A.H.;
"Molecular characterization of the trypanothione reductase gene from Crithidia fasciculata and Trypanosoma brucei: comparison with other flavoprotein disulphide oxidoreductases with respect to substrate specificity and catalytic mechanism.";
Mol. Microbiol. 6:3089-3099(1992).

Comments

FUNCTION: Trypanothione is the parasite analog of glutathione; this enzyme is the equivalent of glutathione reductase.
CATALYTIC ACTIVITY: Trypanothione + NADP⁺ = trypanothione disulfide + NADPH.
COFACTOR: Binds 1 FAD per subunit (By similarity).
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
MISCELLANEOUS: The active site is a redox-active disulfide bond.
SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X63188; CAA44870.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S28003; S28003.

3D structure databases

HSSP

P28593; 1NDA. [HSSP ENTRY / PDB]

SMR

P39051; 2-485.

ModBase

P39051.

Family and domain databases

InterPro

IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR000815; Hg_reductase.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
IPR012999; Pyr_OxRdtase_I_AS.
IPR001327; Pyr_OxRdtase_NAD_bd.
IPR001864; Trypnth_redctse.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.390.30; Pyr_redox_dim; 1.

Pfam

PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
PF02852; Pyr_redox_dim; 1.
Pfam graphical view of domain structure.

PRINTS

PR00368; FADPNR.
PR00945; HGRDTASE.
PR00411; PNDRDTASEI.
PR00470; TRYPANRDTASE.

ProDom

PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR01423; trypano_reduc; 1.

PROSITE

PS00076; PYRIDINE_REDOX_1; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; FAD; Flavoprotein; NADP; Oxidoreductase; Redox-active center.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 492`	`492`	`Trypanothione reductase.`	`PRO_0000067990`
`NP_BIND`	`35 52`	`18`	`FAD (By similarity).`
`ACT_SITE`	`461 461`		`Proton acceptor (By similarity).`
`DISULFID`	`52 57`		`Redox-active (By similarity).`

Sequence information

Length: 492 AA [This is the length of the unprocessed precursor]

Molecular weight: 53284 Da [This is the MW of the unprocessed precursor]

CRC64: FFCA2F11B66F7FF4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSKIFDLVVI GAGSGGLEAG WNAATLYKKR VAVIDVQTHH GPPHYAALGG TCVNVGCVPK 

        70         80         90        100        110        120 
KLMVTGAQYM DHLRESAGFG WEFDGSSVKA NWKKLIAAKN EAVLDINKSY EGMFNDTEGL 

       130        140        150        160        170        180 
DFFLGWGSLE SKNVVVVRET ADPKSAVKER LQADHILLAT GSWPQMPAIP GVEHCISSNE 

       190        200        210        220        230        240 
AFYLPEPPRR VLTVGGGFIS VEFAGIFNAY KPPGGKVTLC YRNNLILRGF DETIREEVTK 

       250        260        270        280        290        300 
QLTANGIEIM TNENPAKVSL NTDGSKHVTF ESGKTLDVDV VMMAIGRIPR TNDLQLGNVG 

       310        320        330        340        350        360 
VKLTPKGGVQ VDEFSRTNVP NIYAIGDITD RLMLTPVAIN EGAALVDTVF GNKPRKTDHT 

       370        380        390        400        410        420 
RVASAVFSIP PIGTCGLIEE VAAKEFEKVA VYMSSFTPLM HNISGSKYKK FVAKIVTNHS 

       430        440        450        460        470        480 
DGTVLGVHLL GDGAPEIIQA VGVCLRLNAK ISDFYNTIGV HPTSAEELCS MRTPSYYYLK 

       490 
GEKMETLPES SL

P39051 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools