[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=1294332 [NCBI, ExPASy, EBI, Israel, Japan] Yagi K., Seto T., Terakado M., Umeda F., Doi T., Imanishi T., Miura Y.; "Nucleotide sequences of membrane-bound hydrogenase gene in Alcaligenes hydrogenophilus."; Chem. Pharm. Bull. 40:3292-3296(1992).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S56898; AAB25779.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JH0775; JH0775.

3D structure databases

HSSP

P18187; 1FRF. [HSSP ENTRY / PDB]

ModBase

P33375.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0051538;	Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0033748;	Molecular function: hydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006137; OxRdtase_q6.
IPR006311; Tat.
Graphical view of domain structure.

Pfam

PF08425; NiFe_dehyd_N; 1.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000310; NiFe_hyd_ssu; 1.

PRINTS

PR00614; NIHGNASESMLL.

TIGRFAMs

TIGR00391; hydA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3Fe-4S; 4Fe-4S; Cell membrane; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 43`	`43`	`Tat-type signal (Potential).`
`CHAIN`	`44 363`	`320`	`Uptake hydrogenase small subunit.`	`PRO_0000013422`
`METAL`	`60 60`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`63 63`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`158 158`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`192 192`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`230 230`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`233 233`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`258 258`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`264 264`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`273 273`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`292 292`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`295 295`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`

Sequence information

Length: 363 AA [This is the length of the unprocessed precursor]

Molecular weight: 39696 Da [This is the MW of the unprocessed precursor]

CRC64: 1327F5062D81A61B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIETFYEVMR RQGISRRSFL KYCSLTATSL GLSPVFVPKI VHAMETKPRT PVLWLHGLEC 

        70         80         90        100        110        120 
TCCSESFIRS AHPLAKDVVL SMISLDYDDT LMAAAGHQAE AILGEVMTKY KGNYILAVEG 

       130        140        150        160        170        180 
NPPLNQDGMS CIIGGKPFID QLRHVAKDAK AIISWGSCAS WGCVQAAKAN PTQATPIHKV 

       190        200        210        220        230        240 
ITDKPIIKVP GCPPIAEVMT GVITYMLTFD RFPELDRQGR PKMFYSQRIH DKCYRRPHFD 

       250        260        270        280        290        300 
AGQFVESWDD ESARKGYCLY KVGCKGPTTY NACSTTRWNG GTSFPIQSGH GCIGCSEDGF 

       310        320        330        340        350        360 
WDKGSFYSRL TNIHQFGIEA NADSVGVTAV GVVGAATAAH AAVSAIKRAR HKDAAQDTAA 


TQK

P33375 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools