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UniProtKB/Swiss-Prot entry P32614


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FRDS_YEAST
Primary accession number P32614
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1993 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 65)
Name and origin of the protein
Protein name Fumarate reductase
Synonyms NADH
EC 1.3.1.6
NADH-dependent fumarate reductase
FAD-dependent oxidoreductase FRDS
Gene name
OrderedLocusNames: YEL047C
ORFNames: SYGP-ORF35
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 89-106; 245-253; 322-327 AND 409-423, AND SUBCELLULAR LOCATION.
DOI=10.1093/dnares/3.4.263; PubMed=8946166 [NCBI, ExPASy, EBI, Israel, Japan]
Enomoto K., Ohki R., Muratsubaki H.;
"Cloning and sequencing of the gene encoding the soluble fumarate reductase from Saccharomyces cerevisiae.";
DNA Res. 3:263-267(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169868 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
Nature 387:78-81(1997).
[3]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[4]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
DOI=10.1073/pnas.2135385100; PubMed=14576278 [NCBI, ExPASy, EBI, Israel, Japan]
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.;
"The proteome of Saccharomyces cerevisiae mitochondria.";
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, AND MASS SPECTROMETRY.
DOI=10.1021/pr060559j; PubMed=17330950 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.;
"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.";
J. Proteome Res. 6:1190-1197(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U18779; AAB64995.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S30830; S30830.
RefSeq NP_010867.1; -.
3D structure databases
HSSP P83223; 1D4D. [HSSP ENTRY / PDB]
ModBase P32614.
Protein-protein interaction databases
DIP DIP:5287N; -.
IntAct P32614; -.
Organism-specific databases
CYGD YEL047c; -.
SGD S000000773; YEL047C.
Yeast-GFP YEL047C.
Gene expression databases
ArrayExpress P32614; -.
GermOnline YEL047C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from SGD).
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0016156; Molecular function: fumarate reductase (NADH) activity (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0008152; Biological process: metabolic process (traceable author statement from SGD).
QuickGo view.
Family and domain databases
InterPro IPR003953; FAD_bind2_N.
IPR010960; Flavocytochrome_c.
Graphical view of domain structure.
Pfam PF00890; FAD_binding_2; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01813; flavo_cyto_c; 1.
BLOCKS P32614.
Proteomic databases
PeptideAtlas P32614; -.
Genome annotation databases
Ensembl YEL047C; Saccharomyces cerevisiae. [Contig view]
GeneID 856664; -.
GenomeReviews U00092_GR; YEL047C.
KEGG sce:YEL047C; -.
NMPDR fig|4932.3.peg.1918; -.
Phylogenomic databases
HOGENOM P32614; -.
Other
LinkHub P32614; -.
ProtoNet P32614.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   470  470     Fumarate reductase. PRO_0000158669
NP_BIND   6    20  15     AMP (Potential). 
ACT_SITE   249   249        By similarity. 
ACT_SITE   272   272        By similarity. 
MOD_RES   66    66        Phosphoserine. 
CONFLICT   285   285        R -> K (in Ref. 1; AA sequence). 
CONFLICT   440   440        V -> L (in Ref. 1; AA sequence). 
Sequence information
Length: 470 AA [This is the length of the unprocessed precursor] Molecular weight: 50844 Da [This is the MW of the unprocessed precursor] CRC64: 63C30B39252DC935 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSLSPVVVIG TGLAGLAAAN ELVNKYNIPV TILEKASSIG GNSIKASSGI NGACTETQRH 

        70         80         90        100        110        120 
FHIEDSPRLF EDDTIKSAKG KGVQELMAKL ANDSPLAIEW LKNEFDLKLD LLAQLGGHSV 

       130        140        150        160        170        180 
ARTHRSSGKL PPGFEIVSAL SNNLKKLAET KPELVKINLD SKVVDIHEKD GSISAVVYED 

       190        200        210        220        230        240 
KNGEKHMVSA NDVVFCSGGF GFSKEMLKEY APELVNLPTT NGQQTTGDGQ RLLQKLGADL 

       250        260        270        280        290        300 
IDMDQIQVHP TGFIDPNDRS SSWKFLAAES LRGLGGILLN PITGRRFVNE LTTRDVVTAA 

       310        320        330        340        350        360 
IQKVCPQEDN RALLVMGEKM YTDLKNNLDF YMFKKLVQKL TLSQVVSEYN LPITVAQLCE 

       370        380        390        400        410        420 
ELQTYSSFTT KADPLGRTVI LNEFGSDVTP ETVVFIGEVT PVVHFTMGGA RINVKAQVIG 

       430        440        450        460        470 
KNDERLLKGL YAAGEVSGGV HGANRLGGSS LLECVVFGRT AAESIANDRK 

P32614 in FASTA format

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