ID   MMSA_PSEAE              Reviewed;         497 AA.
AC   P28810;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   04-NOV-2008, entry version 60.
DE   RecName: Full=Methylmalonate-semialdehyde dehydrogenase [acylating];
DE            Short=MMSDH;
DE            EC=1.2.1.27;
GN   Name=mmsA; OrderedLocusNames=PA3570;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-30.
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=92317087; PubMed=1339433;
RA   Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R.;
RT   "Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO
RT   encoding methylmalonate-semialdehyde dehydrogenase and 3-
RT   hydroxyisobutyrate dehydrogenase.";
RL   J. Biol. Chem. 267:13585-13592(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   PROTEIN SEQUENCE OF 186-197 AND 335-351.
RC   STRAIN=ATCC 33467 / type 1 smooth;
RA   Liddor M.;
RT   "Biofouling in water treatment systems: effect of membrane properties
RT   on biofilm formation.";
RL   Thesis (2005), Ben-Gurion University, Israel.
CC   -!- CATALYTIC ACTIVITY: 2-methyl-3-oxopropanoate + CoA + H(2)O +
CC       NAD(+) = propanoyl-CoA + HCO(3)(-) + NADH.
CC   -!- PATHWAY: Amino-acid degradation; L-valine degradation.
CC   -!- SUBUNIT: Homodimer.
CC   -!- INDUCTION: By valine.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; M84911; AAA25891.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG06958.1; -; Genomic_DNA.
DR   PIR; B42902; B42902.
DR   RefSeq; NP_252260.1; -.
DR   HSSP; P51977; 1BXS.
DR   GeneID; 878814; -.
DR   GenomeReviews; AE004091_GR; PA3570.
DR   KEGG; pae:PA3570; -.
DR   PseudoCAP; PA3570; -.
DR   HOGENOM; P28810; -.
DR   BioCyc; MetaCyc:MON-11663; -.
DR   BioCyc; PAER208964:PA3570-MON; -.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR016160; Ald_DHase_CS.
DR   InterPro; IPR016162; Ald_DHase_N.
DR   InterPro; IPR015590; Aldehyde_DHase.
DR   InterPro; IPR010061; MeMal-semiAld_DHase.
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   PANTHER; PTHR11699:SF27; MMSDH; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   TIGRFAMs; TIGR01722; MMSDH; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    497       Methylmalonate-semialdehyde dehydrogenase
FT                                [acylating].
FT                                /FTId=PRO_0000056584.
FT   ACT_SITE    282    282       By similarity.
SQ   SEQUENCE   497 AA;  53663 MW;  3DB6BFC300AACA4D CRC64;
     MSVPVRHLIA GAFVEGLGAQ RIPVSNPLDN STLAEIACAS AEQVEQAVAS ARETFASWKE
     TPVSERARVM LRYQALLKEH HDELAKIVSS ELGKTFEDAK GDVWRGIEVV EHACNVPSLL
     MGETVENVAR NIDTYSITQP LGVCVGITPF NFPAMIPLWM FPLAIACGNA FILKPSEQVP
     LTSVRLAELF LEAGAPKGVL QVVHGGKEQV DQLLKHPQVK AVSFVGSVAV GQYVYHTGTA
     HNKRVQSFAG AKNHMVIMPD ADKAQVISNL VGASVGAAGQ RCMAISVAVL VGAAREWIPE
     IRDALAKVRP GPWDDSGASY GPVINPQAKA RIERLIGQGV EEGAQLLLDG RGYKVEGYPD
     GNWVGPTLFA GVRPDMAIYR EEVFGPVLCL AEVDSLEQAI RLINESPYGN GTSIFTSSGA
     AARTFQHHIE VGQVGINIPI PVPLPFFSFT GWKGSFYGDL HAYGKQGVRF YTETKTVTAR
     WFDSDSVAGT NFSIQMR
//