[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). STRAIN=Sprague-Dawley; TISSUE=Brain; PubMed=1353889 [NCBI, ExPASy, EBI, Israel, Japan] Guastella J., Brecha N., Weigmann C., Lester H.A., Davidson N.; "Cloning, expression, and localization of a rat brain high-affinity glycine transporter."; Proc. Natl. Acad. Sci. U.S.A. 89:7189-7193(1992).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). TISSUE=Brain; DOI=10.1016/0896-6273(92)90207-T; PubMed=1534013 [NCBI, ExPASy, EBI, Israel, Japan] Smith K.E., Borden L.A., Hartig P.R., Branchek T.A., Weinshank R.L.; "Cloning and expression of a glycine transporter reveal colocalization with NMDA receptors."; Neuron 8:927-935(1992).
[3]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). TISSUE=Blood; DOI=10.1016/0896-6273(93)90201-2; PubMed=8494645 [NCBI, ExPASy, EBI, Israel, Japan] Borowsky B., Mezey E., Hoffman B.J.; "Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene."; Neuron 10:851-863(1993).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). TISSUE=Brain; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.
INTERACTION:
P31016:Dlg4; NbExp=2; IntAct=EBI-848783, EBI-375655;
P31016:Dlg4; NbExp=2; IntAct=EBI-848796, EBI-375655;
P18614:Itga1; NbExp=1; IntAct=EBI-848783, EBI-848806;
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Synonyms	GlyT-1A, GlyT-2
Isoform ID	P28572-1
This is the isoform sequence displayed in this entry.

Name	2
Synonyms	GlyT-1B, GlyT-1
Isoform ID	P28572-2
Features which should be applied to build the isoform sequence: VSP_006273.

TISSUE SPECIFICITY: GLYT-1A is found in the gray matter of CNS as well as in macrophages and mast cells in peripherical tissues. GLYT-1B is only found in the white matter of CNS.
SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M95413; AAA41256.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M88595; AAA41257.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L13600; AAA73557.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC128732; AAI28733.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

I58140; I58140.
JH0673; JH0673.

RefSeq

NP_446270.1; -.

UniGene

Rn.32110

3D structure databases

ModBase

P28572.

Protein-protein interaction databases

IntAct

P28572; -.

Organism-specific databases

RGD

621243; Slc6a9.

Gene expression databases

ArrayExpress

P28572; -.

GermOnline

ENSRNOG00000019484; Rattus norvegicus.

Ontologies

GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.

Family and domain databases

InterPro

IPR000175; Na/ntran_symport.
IPR003028; Na/ntran_symport_glycine_GLY1.
Graphical view of domain structure.

PANTHER

PTHR11616; Na/ntran_symport; 1.

Pfam

PF00209; SNF; 1.
Pfam graphical view of domain structure.

PRINTS

PR01204; GLY1TRNSPORT.
PR00176; NANEUSMPORT.

ProDom

PD000448; Na/ntran_symport; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00610; NA_NEUROTRAN_SYMP_1; 1.
PS00754; NA_NEUROTRAN_SYMP_2; 1.
PS50267; NA_NEUROTRAN_SYMP_3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P28572.

Genome annotation databases

Ensembl

ENSRNOG00000019484; Rattus norvegicus. [Contig view]

GeneID

116509; -.

KEGG

rno:116509; -.

Phylogenomic databases

HOVERGEN

P28572; -.

Other

ProtoNet

P28572.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Amino-acid transport; Glycoprotein; Membrane; Neurotransmitter transport; Symport; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 633`	`633`	`Sodium- and chloride-dependent glycine transporter 1.`	`PRO_0000214782`
`TOPO_DOM`	`1 35`	`35`	`Cytoplasmic (Potential).`
`TRANSMEM`	`36 56`	`21`	`1 (Potential).`
`TRANSMEM`	`64 83`	`20`	`2 (Potential).`
`TRANSMEM`	`107 127`	`21`	`3 (Potential).`
`TOPO_DOM`	`128 214`	`87`	`Extracellular (Potential).`
`TRANSMEM`	`215 233`	`19`	`4 (Potential).`
`TRANSMEM`	`242 259`	`18`	`5 (Potential).`
`TRANSMEM`	`295 312`	`18`	`6 (Potential).`
`TRANSMEM`	`324 345`	`22`	`7 (Potential).`
`TRANSMEM`	`378 397`	`20`	`8 (Potential).`
`TRANSMEM`	`426 444`	`19`	`9 (Potential).`
`TRANSMEM`	`460 480`	`21`	`10 (Potential).`
`TRANSMEM`	`501 520`	`20`	`11 (Potential).`
`TRANSMEM`	`539 557`	`19`	`12 (Potential).`
`TOPO_DOM`	`558 633`	`76`	`Cytoplasmic (Potential).`
`CARBOHYD`	`164 164`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`167 167`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`177 177`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`183 183`		`N-linked (GlcNAc...) (Potential).`
`VAR_SEQ`	`1 10`		`MVGKGAKGML -> MAVAHGPVATSSPEQ (in isoform 2).`	`VSP_006273`

Sequence information

Length: 633 AA [This is the length of the unprocessed precursor]

Molecular weight: 70571 Da [This is the MW of the unprocessed precursor]

CRC64: 7D25B555B97E9E17 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVGKGAKGML NGAVPSEATK KDQNLTRGNW GNQIEFVLTS VGYAVGLGNV WRFPYLCYRN 

        70         80         90        100        110        120 
GGGAFMFPYF IMLVFCGIPL FFMELSFGQF ASQGCLGVWR ISPMFKGVGY GMMVVSTYIG 

       130        140        150        160        170        180 
IYYNVVICIA FYYFFSSMTH VLPWAYCNNP WNTPDCAGVL DASNLTNGSR PTALSGNLSH 

       190        200        210        220        230        240 
LFNYTLQRTS PSEEYWRLYV LKLSDDIGDF GEVRLPLLGC LGVSWVVVFL CLIRGVKSSG 

       250        260        270        280        290        300 
KVVYFTATFP YVVLTILFVR GVTLEGAFTG IMYYLTPKWD KILEAKVWGD AASQIFYSLG 

       310        320        330        340        350        360 
CAWGGLITMA SYNKFHNNCY RDSVIISITN CATSVYAGFV IFSILGFMAN HLGVDVSRVA 

       370        380        390        400        410        420 
DHGPGLAFVA YPEALTLLPI SPLWSLLFFF MLILLGLGTQ FCLLETLVTA IVDEVGNEWI 

       430        440        450        460        470        480 
LQKKTYVTLG VAVAGFLLGI PLTSQAGIYW LLLMDNYAAS FSLVVISCIM CVSIMYIYGH 

       490        500        510        520        530        540 
RNYFQDIQMM LGFPPPLFFQ ICWRFVSPTI IFFILIFTVI QYRPITYNHY QYPGWAVAIG 

       550        560        570        580        590        600 
FLMALSSVIC IPLYALFQLC RTDGDTLLQR LKNATKPSRD WGPALLEHRT GRYAPTTTPS 

       610        620        630 
PEDGFEVQPL HPDKAQIPIV GSNGSSRLQD SRI

P28572 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools