ID   PYRD_AGRAE              Reviewed;         328 AA.
AC   P28294;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   04-NOV-2008, entry version 60.
DE   RecName: Full=Dihydroorotate dehydrogenase;
DE            Short=DHOdehase;
DE            Short=DHODase;
DE            Short=DHOD;
DE            EC=1.3.3.1;
DE   AltName: Full=Dihydroorotate oxidase;
GN   Name=URA1;
OS   Agrocybe aegerita (Black poplar mushroom).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
OC   Agaricomycetes; Agaricomycetidae; Agaricales; Bolbitiaceae; Agrocybe.
OX   NCBI_TaxID=5400;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=WT-2 / G30;
RX   MEDLINE=93083991; PubMed=1452035; DOI=10.1016/0378-1119(92)90057-V;
RA   Noel T., Labarere J.;
RT   "Sequence of the URA1 gene encoding dihydroorotate dehydrogenase from
RT   the basidiomycete fungus Agrocybe aegerita.";
RL   Gene 122:233-234(1992).
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + O(2) = orotate +
CC       H(2)O(2).
CC   -!- COFACTOR: Binds 1 FAD per subunit.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 4/6.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the dihydroorotate dehydrogenase family.
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DR   EMBL; M90295; AAA32636.1; -; Genomic_DNA.
DR   PIR; JN0453; JN0453.
DR   HSSP; Q02127; 1D3G.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR012135; DHO_DHase_1_2.
DR   InterPro; IPR005719; DHO_DHase_2.
DR   InterPro; IPR001295; Dihydroorotate_DHase_core.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF01180; DHO_dh; 1.
DR   PIRSF; PIRSF000164; DHO_oxidase; 1.
DR   TIGRFAMs; TIGR01036; pyrD_sub2; 1.
DR   PROSITE; PS00911; DHODEHASE_1; 1.
DR   PROSITE; PS00912; DHODEHASE_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; Oxidoreductase; Pyrimidine biosynthesis.
FT   CHAIN         1    328       Dihydroorotate dehydrogenase.
FT                                /FTId=PRO_0000148499.
FT   ACT_SITE    173    173       Nucleophile (By similarity).
SQ   SEQUENCE   328 AA;  35085 MW;  71FA3D2A4D57EF75 CRC64;
     MSGLFETLGR RALFAFDAEQ AHGLSIAGLK TGLVTGSAPN DLALSVKVAG LQFPNPLGMA
     AGYDKNAEVP DALLKLGFGF TEIGTITPRP QSGNPRPRIF RLVDDKAVIN RLGFNNEGHD
     AAFKRLSQRA GKSGIVGVNI GANKDAEDRI ADYVAGIRRF YQLARYFTVN ISSPNTPGLR
     NLQARDSLRE LLSRVLEARN EEGKMCTLKR PVFLKIAPDL ANEELDDIAA EATEQKLDGI
     IISNTTLSRA GLKSAGGRDE TGGLSGAPLF DRSTIVLARM RERVGPDMPL IGVGGVDSAE
     TALTKVKAGA RSRPALYGPD LSRPEPAY
//