NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Triumph;
TISSUE=Testa;
AGRICOLA=IND92043518
Meldgaard M.;
"Expression of chalcone synthase, dihydroflavonol reductase and flavanone-3-hydroxylase in mutants of barley deficient in anthocyanin and proantocyanidin biosynthesis.";
Theor. Appl. Genet. 83:695-706(1992).

Comments

FUNCTION: Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O₂ = a dihydroflavonol + succinate + CO₂.
COFACTOR: Iron.
COFACTOR: Ascorbate.
PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis .
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X58138; CAA41146.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S14647; S14647.

3D structure databases

HSSP

Q96323; 1GP6. [HSSP ENTRY / PDB]

ModBase

P28038.

Organism-specific databases

Gramene

P28038; -.

Ontologies

GO:0045486;	Molecular function: naringenin 3-dioxygenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Oxidoreductase; Vitamin C.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 377`	`377`	`Naringenin,2-oxoglutarate 3-dioxygenase.`	`PRO_0000067286`
`METAL`	`78 78`		`Iron (Potential).`
`METAL`	`220 220`		`Iron (Potential).`
`METAL`	`222 222`		`Iron (Potential).`
`METAL`	`278 278`		`Iron (Potential).`

Sequence information

Length: 377 AA [This is the length of the unprocessed precursor]

Molecular weight: 42057 Da [This is the MW of the unprocessed precursor]

CRC64: 13813A85BB826EF0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAPVSNETFL PTEAWGEATL RPSFVRDEDE RPKVAHDRFS DAVPLISLHG IDGARRAQIR 

        70         80         90        100        110        120 
DRVAAACEDW GIFQVIDHGV DADLIADMTR LAREFFALPA EDKLRYDMSG GKKGGFIVSS 

       130        140        150        160        170        180 
HLQGEAVQDW REIVTYFSYP VKARDYGRWP EKPAGWCAVV ERYSERLMGL SCNLMGVLSE 

       190        200        210        220        230        240 
AMGLETEALA KACVDMDQKV VVNFYPRCPQ PDLTLGLKRH TDPGTITLLL QDLVGGLQAT 

       250        260        270        280        290        300 
RDGGKNWITV QPISGAFVVN LGDHGHFMSN GRFKNADHQA VVNGESSRLS IATFQNPAPD 

       310        320        330        340        350        360 
ARVWPLAVRE GEEPILEEPI TFTEMYRRKM ERDLDLAKRK KQAKDQLMQQ QLQLQQQQAV 

       370 
AAAPMPTATK PLNEILA

P28038 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools