NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=NBI1028;
DOI=10.1016/0167-4781(91)90066-U; PubMed=2001402 [NCBI, ExPASy, EBI, Israel, Japan]
Tamaki T., Fukaya M., Takemura H., Tayama K., Okumura H., Kawamura Y., Nishiyama M., Horinouchi S., Beppu T.;
"Cloning and sequencing of the gene cluster encoding two subunits of membrane-bound alcohol dehydrogenase from Acetobacter polyoxogenes.";
Biochim. Biophys. Acta 1088:292-300(1991).

Comments

FUNCTION: Dehydration of primary alcohols (exception: methanol).
CATALYTIC ACTIVITY: A primary alcohol + acceptor = an aldehyde + reduced acceptor.
COFACTOR: Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-143-Cys-144 and the indole ring of Trp-280 (By similarity).
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
COFACTOR: Binds 1 heme group per subunit (By similarity).
SUBUNIT: Heterotetramer (dehydrogenase, cytochrome, and two smaller unknown subunits) that forms the alcohol dehydrogenase complex.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Periplasmic side (Potential).
SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family.
SIMILARITY: Contains 1 cytochrome c domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D00635; BAA00528.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S14270; S14270.

3D structure databases

HSSP

Q46444; 1KB0. [HSSP ENTRY / PDB]

ModBase

P28036.

Family and domain databases

InterPro

IPR009056; Cyt_c_monohaem.
IPR017512; PQQ_MeOH/EtOH_DH.
IPR002372; PQQ_repeat.
IPR011047; Quino_AlcDHase-like.
IPR001479; Quinoprotein_DHase_PQQ_bac_CS.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.760.10; Cytochrome_c_R; 1.
G3DSA:2.140.10.10; Quinoprotein_alc_DH-like; 1.

Pfam

PF01011; PQQ; 6.
Pfam graphical view of domain structure.

SMART

SM00564; PQQ; 6.
SMART graphical view of domain structure.

PROSITE

PS00363; BACTERIAL_PQQ_1; 1.
PS00364; BACTERIAL_PQQ_2; 1.
PS51007; CYTC; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Cell membrane; Direct protein sequencing; Heme; Iron; Membrane; Metal-binding; Oxidoreductase; Periplasm; PQQ; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 35`	`35`	`Potential.`
`CHAIN`	`36 738`	`703`	`Alcohol dehydrogenase [acceptor].`	`PRO_0000025561`
`DOMAIN`	`634 738`	`105`	`Cytochrome c.`
`ACT_SITE`	`343 343`		`Proton acceptor (Potential).`
`METAL`	`217 217`		`Calcium (By similarity).`
`METAL`	`298 298`		`Calcium (By similarity).`
`METAL`	`654 654`		`Iron (heme axial ligand) (By similarity).`
`BINDING`	`650 650`		`Heme (covalent) (By similarity).`
`BINDING`	`653 653`		`Heme (covalent) (By similarity).`
`DISULFID`	`143 144`		`By similarity.`

Sequence information

Length: 738 AA [This is the length of the unprocessed precursor]

Molecular weight: 80841 Da [This is the MW of the unprocessed precursor]

CRC64: 1E2B6ED7BCD92AF6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MISAVFGKRR SLSRTLTAGT ICAALISGYA TMASADDGQG ATGEAIIHAD DHPGNWMTYG 

        70         80         90        100        110        120 
RTYSDQRYSP LDQINRSNVG NLKLAWYLDL DTNRGQEGTP LVIDGVMYAT TNWSMMKAVD 

       130        140        150        160        170        180 
AATGKLLWSY DPRVPGNIAD KGCCDTVNRG AAYWNGKVYF GTFDGRLIAL DAKTGKLVWS 

       190        200        210        220        230        240 
VNTIPPEAEL GKQRSYTVDG APRIAKGRVI IGNGGSEFGA RGFVSAFDAE TGKVDWRFFT 

       250        260        270        280        290        300 
VPNPKNEPDA ASDSVLMNKA YQTWSPTGAW TRQGGGGTVW DSIVYDPVAD LVYLGVGNGS 

       310        320        330        340        350        360 
PWNYKYRSEG KGDNLFLGSI VALKPETGEY VWHFQETPMD QWDFTSDQQI MTLDLPINGE 

       370        380        390        400        410        420 
TRHVIVHARK NGFFYIIDAK TGEFISGKNY VYVNWASGLD PKTGRPIYNP DALYTLTGKE 

       430        440        450        460        470        480 
WYGIPGDLGG HNFAAMAFSP KTGLVYIPAQ QVPFLYTNQV GGFTPHPDSW NLGLDMNKVG 

       490        500        510        520        530        540 
IPDSPEAKQA FVKDLKGWIV AWDPQKQAEA WRVDHKGPWN GGILATGGDL LFQGLANGEF 

       550        560        570        580        590        600 
HAYDATNGSD LFHFAADSGI IAPPVTYLAN GKQYVAVEVG WGGIYPFFLG GLARTSGWTV 

       610        620        630        640        650        660 
NHSRIIAFSL DGKSGPLPKQ NDQGFLPVKP PAQFDSKRTD NGYFQFQTYC AACHGDNAEG 

       670        680        690        700        710        720 
AGVLPDLRWS GSIRHEDAFY NVVGRGALTA YGMDRLHGNM NPTEIEDIRQ FLIKRANETY 

       730 
QREVDARKNA DGIPEQLP

P28036 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools