[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1038/357080a0; PubMed=1374165 [NCBI, ExPASy, EBI, Israel, Japan] Armitage R., Fanslow W., Sato T.A., Clifford K.N., Strockbine L., Macduff B.M., Anderson D.M., Gimpel S.D., Davis-Smith T., Maliszewski C.R., Clark E.A., Smith C.A., Grabstein K.H., Cosman D., Spriggs M.K.; "Molecular and biological characterization of a murine ligand for CD40."; Nature 357:80-82(1992).
[2]	SEQUENCE REVISION TO 198. Spriggs M.K.; Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 137-260. STRAIN=129/Sv; TISSUE=Liver; DOI=10.1016/0161-5890(94)90009-4; PubMed=7520529 [NCBI, ExPASy, EBI, Israel, Japan] Tsitsikov E.N., Ramesh N., Geha R.S.; "Structure of the murine CD40 ligand gene."; Mol. Immunol. 31:895-900(1994).
[4]	SIMILARITY TO THE TNF FAMILY. DOI=10.1038/358026b0; PubMed=1377364 [NCBI, ExPASy, EBI, Israel, Japan] Farrah T., Smith C.A.; "Emerging cytokine family."; Nature 358:26-26(1992).
[5]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-4; SER-6 AND SER-149, AND MASS SPECTROMETRY. TISSUE=Liver; DOI=10.1021/pr0604155; PubMed=17203969 [NCBI, ExPASy, EBI, Israel, Japan] Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry."; J. Proteome Res. 6:250-262(2007).
[6]	3D-STRUCTURE MODELING OF 115-260, AND DISULFIDE BOND. DOI=10.1093/intimm/5.2.233; PubMed=8095800 [NCBI, ExPASy, EBI, Israel, Japan] Peitsch M.C., Jongeneel C.V.; "A 3-D model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors."; Int. Immunol. 5:233-238(1993).

Comments

FUNCTION: Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching (By similarity).
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein.
SUBCELLULAR LOCATION: CD40 ligand, soluble form: Secreted.
TISSUE SPECIFICITY: Specifically expressed on activated CD4+ T-lymphocytes.
PTM: The soluble form derives from the membrane form by proteolytic processing (By similarity).
SIMILARITY: Belongs to the tumor necrosis factor family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X65453; CAA46448.2; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
S71858; AAC13639.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
S71861; AAC13640.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S21738; S21738.

NP_035746.2; -.

3D structure databases

PDB

1CDA; Model; -; A/B/C=115-260.

[ExPASy / RCSB / EBI]

1CDA; -.

P27548; 115-260.

PTM databases

P27548; -.

Organism-specific databases

MGI

MGI:88337; Cd40lg.

Gene expression databases

ArrayExpress

P27548; -.

CleanEx

MM_CD40LG; -.

GermOnline

ENSMUSG00000031132; Mus musculus.

Ontologies

GO:0005174;	Molecular function: CD40 receptor binding (inferred from sequence or structural similarity from UniProtKB).
GO:0006916;	Biological process: anti-apoptosis (inferred from sequence or structural similarity from UniProtKB).
GO:0030183;	Biological process: B cell differentiation (inferred from genetic interaction from MGI).
GO:0042100;	Biological process: B cell proliferation (inferred from sequence or structural similarity from UniProtKB).
GO:0006954;	Biological process: inflammatory response (inferred from sequence or structural similarity from UniProtKB).
GO:0045190;	Biological process: isotype switching (inferred from direct assay from UniProtKB).
GO:0030168;	Biological process: platelet activation (inferred from sequence or structural similarity from UniProtKB).
GO:0051023;	Biological process: regulation of immunoglobulin secretion (inferred from direct assay from MGI).
GO:0007165;	Biological process: signal transduction (non-traceable author statement from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR003263; TNF_5.
IPR006052; TNF_family.
IPR008983; Tumour_necrosis_fac-like.
Graphical view of domain structure.

Gene3D

G3DSA:2.60.120.40; Tumour_necrosis_fac-like; 1.

Pfam

PF00229; TNF; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF016527; TNF_5; 1.

PRINTS

PR01702; CD40LIGAND.

ProDom

PD002012; TNF_subf; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00207; TNF; 1.
SMART graphical view of domain structure.

PROSITE

PS00251; TNF_1; 1.
PS50049; TNF_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P27548.

Genome annotation databases

Ensembl

ENSMUSG00000031132; Mus musculus. [Contig view]

GeneID

21947; -.

KEGG

mmu:21947; -.

Phylogenomic databases

HOGENOM

P27548; -.

HOVERGEN

P27548; -.

Other

SOURCE

Cd40lg; Mus musculus.

ProtoNet

P27548.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Cell membrane; Cytokine; Glycoprotein; Membrane; Phosphoprotein; Secreted; Signal-anchor; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 260`	`260`	`CD40 ligand, membrane form.`	`PRO_0000034490`
`CHAIN`	`112 260`	`149`	`CD40 ligand, soluble form (By similarity).`	`PRO_0000034491`
`TOPO_DOM`	`1 22`	`22`	`Cytoplasmic (Potential).`
`TRANSMEM`	`23 46`	`24`	`Signal-anchor for type II membrane protein (Potential).`
`TOPO_DOM`	`47 260`	`214`	`Extracellular (Potential).`
`SITE`	`111 112`	`2`	`Cleavage (By similarity).`
`MOD_RES`	`4 4`		`Phosphothreonine.`
`MOD_RES`	`6 6`		`Phosphoserine.`
`MOD_RES`	`149 149`		`Phosphoserine.`
`CARBOHYD`	`239 239`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`177 217`
`CONFLICT`	`198 198`		`S -> I (in Ref. 3; AAC13640).`
`STRAND`	`118 120`	`3`
`STRAND`	`122 127`	`6`
`TURN`	`130 132`	`3`
`TURN`	`156 158`	`3`
`STRAND`	`159 161`	`3`
`STRAND`	`166 176`	`11`
`STRAND`	`188 195`	`8`
`STRAND`	`202 209`	`8`
`STRAND`	`220 230`	`11`
`STRAND`	`235 242`	`8`
`STRAND`	`253 260`	`8`

Sequence information

Length: 260 AA [This is the length of the unprocessed precursor]

Molecular weight: 29370 Da [This is the MW of the unprocessed precursor]

CRC64: 7E1AC117473672AD [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL DKVEEEVNLH 

        70         80         90        100        110        120 
EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL NKEEKKENSF EMQRGDEDPQ 

       130        140        150        160        170        180 
IAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVMLENGKQL TVKREGLYYV YTQVTFCSNR 

       190        200        210        220        230        240 
EPSSQRPFIV GLWLKPSSGS ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV 

       250        260 
TEASQVIHRV GFSSFGLLKL

P27548 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools