CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD⁺ = 3-phospho-D-glyceroyl phosphate + NADH.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5 .
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Copyright

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Cross-references

Sequence databases

EMBL

X60344; CAA42902.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S18484; DEPZG.

3D structure databases

HSSP

P56649; 1IHX. [HSSP ENTRY / PDB]

ModBase

P26519.

Family and domain databases

InterPro

IPR000173; GlycerAld_3-P_DHase.
IPR006424; Glyceraldehyde-3-P_DHase_1.
Graphical view of domain structure.

PANTHER

PTHR10836; GAP_DH; 1.

Pfam

PF02800; Gp_dh_C; 1.
PF00044; Gp_dh_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000149; GAP_DH; 1.

PRINTS

PR00078; G3PDHDRGNASE.

TIGRFAMs

TIGR01534; GAPDH-I; 1.

PS00071; GAPDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Glycolysis; NAD; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 336`	`336`	`Glyceraldehyde-3-phosphate dehydrogenase, cytosolic.`	`PRO_0000145611`
`NP_BIND`	`12 13`	`2`	`NAD (By similarity).`
`REGION`	`152 154`	`3`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`REGION`	`212 213`	`2`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`ACT_SITE`	`153 153`		`Nucleophile (By similarity).`
`BINDING`	`34 34`		`NAD (By similarity).`
`BINDING`	`81 81`		`NAD; via carbonyl oxygen (By similarity).`
`BINDING`	`183 183`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`235 235`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`317 317`		`NAD (By similarity).`
`SITE`	`180 180`	`1`	`Activates thiol group during catalysis (By similarity).`

Sequence information

Length: 336 AA [This is the length of the unprocessed precursor]

Molecular weight: 36372 Da [This is the MW of the unprocessed precursor]

CRC64: 9CD52A143AFA2494 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKMKIGINGF GRIGRLVARV ALMSDDIELV AVNDPFITTE YMTYMFKYDS VHGQWKKDEL 

        70         80         90        100        110        120 
KVKDSKTLLF GDKPLTVFGV RNPEEDPWGE AGAEYVVEST GVFTDKDKAA AHLKGGAKKV 

       130        140        150        160        170        180 
VISAPSGNAP MFVVGVNEKE YKKDIDIVSN ASCTTNCLAP LAKVLNDKFG IVEGLMTTVH 

       190        200        210        220        230        240 
SITATRKTVD GPSMKDWRGG RAASFNIIPS STGAAKAVGK VLPALNGKLT GMAFRVPTVD 

       250        260        270        280        290        300 
VSVVDLTARL EKAATYDEIK AAIKHESETS LKGILGYTED DVVSTDFVGD SRSSIFDAKA 

       310        320        330 
GIALNGNFVK VVSWYDNEWG YSNRVIDLIR HMASVA

P26519 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools