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UniProtKB/Swiss-Prot entry P24404


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_AGRT5
Primary accession number P24404
Secondary accession numbers None
Integrated into Swiss-Prot on March 1, 1992
Sequence was last modified on March 27, 2002 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 69)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms EC 1.1.1.85
Beta-IPM dehydrogenase
IMDH
3-IPM-DH
Gene name
Name: leuB
OrderedLocusNames: Atu2791
ORFNames: AGR_C_5067
From
Agrobacterium tumefaciens (strain C58 / ATCC 33970) [TaxID: 176299] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strizhov N.I., Kryukov V.M., Bur'Yanov Y.I., Beav A.A.;
"Beta-isopropyl malate dehydrogenase gene of Agrobacterium tumefaciens C58: coding and primary structure.";
Dokl. Akad. Nauk SSSR 288:481-486(1986).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1126/science.1066804; PubMed=11743193 [NCBI, ExPASy, EBI, Israel, Japan]
Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W.;
"The genome of the natural genetic engineer Agrobacterium tumefaciens C58.";
Science 294:2317-2323(2001).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1126/science.1066803; PubMed=11743194 [NCBI, ExPASy, EBI, Israel, Japan]
Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58.";
Science 294:2323-2328(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M38670; AAA22089.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE007869; AAK88504.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR AF2919; AF2919.
G97693; G97693.
RefSeq NP_355719.1; -.
3D structure databases
HSSP Q56268; 1A05. [HSSP ENTRY / PDB]
ModBase P24404.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_01033; -; 1.
PBIL [Tree]
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS P24404.
Genome annotation databases
GeneID 1134829; -.
GenomeReviews AE007869_GR; Atu2791.
KEGG atc:AGR_C_5067; -.
atu:Atu2791; -.
Phylogenomic databases
HOGENOM P24404; -.
Genome annotation databases
CMR P24404; Atu2791.
Other
ProtoNet P24404.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   370  370     3-isopropylmalate dehydrogenase. PRO_0000083627
NP_BIND   77    90  14     NAD (By similarity). 
NP_BIND   290   302  13     NAD (By similarity). 
METAL   226   226        Magnesium or manganese (By similarity). 
METAL   250   250        Magnesium or manganese (By similarity). 
METAL   254   254        Magnesium or manganese (By similarity). 
BINDING   97    97        Substrate (By similarity). 
BINDING   107   107        Substrate (By similarity). 
BINDING   135   135        Substrate (By similarity). 
BINDING   226   226        Substrate (By similarity). 
SITE   142   142  1     Important for catalysis (By similarity). 
SITE   193   193  1     Important for catalysis (By similarity). 
CONFLICT   6     6        L -> V (in Ref. 1; AAA22089). 
Sequence information
Length: 370 AA [This is the length of the unprocessed precursor] Molecular weight: 39659 Da [This is the MW of the unprocessed precursor] CRC64: BB4138D257849E0B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTVRSLFLLP GDGIGPEAMT EVRKLIEYMN SAHNAGFTVS EGLVGGSAYD AHGVAISDAD 

        70         80         90        100        110        120 
MEKALAADAI LFGAVGGPKW DGVPYEHRPE AGLLRLRKDL ELFANLRPAI CYPALAAASS 

       130        140        150        160        170        180 
LKPELVEGLD ILIVRELTGG VYFGEPKQII DLGNGQKRGI DTQIYDTFEI ERIASVAFEL 

       190        200        210        220        230        240 
ARSRDNRVCS MEKRNVMKSG VLWNQVVTET HAAKYKDVQL EHMLADAGGM QLVRKPKQFD 

       250        260        270        280        290        300 
VIVTDNLFGD MLSDVAAMLT GSLGMLPSAS LGAPDAKTGK RKAMYEPVHG SAPDIAGKSI 

       310        320        330        340        350        360 
ANPIAMIASF AMCLRYSFNM VDEATKLEAA IANVLDKGIR TADIMADGCR QVGTSDMGDA 

       370 
VLAEFKALSA 

P24404 in FASTA format

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