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UniProtKB/Swiss-Prot entry P22637

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CHOD_BREST
Primary accession number P22637
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on May 1, 1992 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 73)
Name and origin of the protein
Protein name Cholesterol oxidase [Precursor]
Synonyms CHOD
EC 1.1.3.6
Gene name
Name: choB
From
Brevibacterium sterolicum [TaxID: 1702] 
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Micrococcineae; Brevibacteriaceae; Brevibacterium.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 46-55.
STRAIN=ATCC 21387 / NCIB 11161;
DOI=10.1016/0378-1119(91)90397-T; PubMed=1879703 [NCBI, ExPASy, EBI, Israel, Japan]
Ohta T., Fujishiro K., Yamaguchi K., Tamura Y., Aisaka K., Uwajima T., Hasegawa M.;
"Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterolicum: comparison with choA of streptomyces sp. SA-COO.";
Gene 103:93-96(1991).
[2]
 PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-552, AND PARTIAL PROTEIN SEQUENCE.
STRAIN=ATCC 21387 / NCIB 11161;
DOI=10.1016/0006-291X(90)90734-5; PubMed=2271066 [NCBI, ExPASy, EBI, Israel, Japan]
Fujishiro K., Ota T., Hasegawa M., Yamaguchi K., Mizukami T., Uwajima T.;
"Isolation and identification of the gene of cholesterol oxidase from Brevibacterium sterolicum ATCC 21387, a widely used enzyme in clinical analysis.";
Biochem. Biophys. Res. Commun. 172:721-727(1990).
[3]
 ERRATUM.
Fujishiro K., Ota T., Hasegawa M., Yamaguchi K., Mizukami T., Uwajima T.;
Biochem. Biophys. Res. Commun. 173:1384-1384(1990).
[4]
 X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
DOI=10.1016/0022-2836(91)90192-9; PubMed=2051487 [NCBI, ExPASy, EBI, Israel, Japan]
Vrielink A., Lloyld L.F., Blow D.M.;
"Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8-A resolution.";
J. Mol. Biol. 219:533-554(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D00712; BAA00617.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JQ1193; JQ1193.
3D structure databases
PDB
1COY; X-ray; 1.80 A; A=46-552.[ExPASy / RCSB / EBI]
3COX; X-ray; 1.80 A; A=46-552.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1COY; -.
3COX; -.
ModBase P22637.
Ontologies
GO
GO:0016995; Molecular function: cholesterol oxidase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR000172; GMC_OxRdtase_N.
IPR002938; mOase_FAD_bd.
IPR006311; Tat.
Graphical view of domain structure.
Pfam PF01494; FAD_binding_3; 1.
Pfam graphical view of domain structure.
PRINTS PR00368; FADPNR.
TIGRFAMs TIGR01409; TAT_signal_seq; 1.
PROSITE PS00623; GMC_OXRED_1; 1.
PS00624; GMC_OXRED_2; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P22637.
Other
LinkHub P22637; -.
ProtoNet P22637.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cholesterol metabolism; Direct protein sequencing; FAD; Flavoprotein; Lipid metabolism; Oxidoreductase; Secreted; Signal; Steroid metabolism.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    45  45     Tat-type signal. 
CHAIN   46   552  507     Cholesterol oxidase. PRO_0000012332
NP_BIND   63    79  17     FAD. 
ACT_SITE   406   406        Proton acceptor. 
ACT_SITE   492   492        By similarity. 
STRAND   56    62  7      
HELIX   66    77  12      
STRAND   82    85  4      
STRAND   104   106  3      
STRAND   111   114  4      
STRAND   140   145  6      
STRAND   150   154  5      
HELIX   159   162  4      
HELIX   173   179  7      
HELIX   185   190  6      
HELIX   192   200  9      
HELIX   207   212  6      
HELIX   214   216  3      
HELIX   217   228  12      
STRAND   233   235  3      
STRAND   238   240  3      
HELIX   242   249  8      
TURN   257   260  4      
STRAND   267   270  4      
TURN   273   276  4      
HELIX   277   283  7      
STRAND   287   290  4      
STRAND   292   300  9      
STRAND   302   313  12      
STRAND   319   332  14      
HELIX   335   348  14      
TURN   357   360  4      
STRAND   368   374  7      
STRAND   391   394  4      
STRAND   403   408  6      
STRAND   418   425  8      
STRAND   433   436  4      
TURN   437   440  4      
STRAND   441   444  4      
HELIX   448   451  4      
HELIX   452   469  18      
STRAND   484   486  3      
STRAND   488   491  4      
TURN   499   501  3      
STRAND   515   517  3      
HELIX   520   522  3      
HELIX   532   549  18      
Sequence information
Length: 552 AA [This is the length of the unprocessed precursor] Molecular weight: 59358 Da [This is the MW of the unprocessed precursor] CRC64: 74913FAED74B4F09 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTDSRANRAD ATRGVASVSR RRFLAGAGLT AGAIALSSMS TSASAAPSRT LADGDRVPAL 

        70         80         90        100        110        120 
VIGSGYGGAV AALRLTQAGI PTQIVEMGRS WDTPGSDGKI FCGMLNPDKR SMWLADKTDQ 

       130        140        150        160        170        180 
PVSNFMGFGI NKSIDRYVGV LDSERFSGIK VYQGRGVGGG SLVNGGMAVT PKRNYFEEIL 

       190        200        210        220        230        240 
PSVDSNEMYN KYFPRANTGL GVNNIDQAWF ESTEWYKFAR TGRKTAQRSG FTTAFVPNVY 

       250        260        270        280        290        300 
DFEYMKKEAA GQVTKSGLGG EVIYGNNAGK KSLDKTYLAQ AAATGKLTIT TLHRVTKVAP 

       310        320        330        340        350        360 
ATGSGYSVTM EQIDEQGNVV ATKVVTADRV FFAAGSVGTS KLLVSMKAQG HLPNLSSQVG 

       370        380        390        400        410        420 
EGWGNNGNIM VGRANHMWDA TGSKQATIPT MGIDNWADPT APIFAEIAPL PAGLETYVSL 

       430        440        450        460        470        480 
YLAITKNPER ARFQFNSGTG KVDLTWAQSQ NQKGIDMAKK VFDKINQKEG TIYRTDLFGV 

       490        500        510        520        530        540 
YFKTWGDDFT YHPLGGVLLN KATDNFGRLP EYPGLYVVDG SLVPGNVGVN PFVTITRLAE 

       550 
RNMDKIISSD IQ
P22637 in FASTA format

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