ID   PCYB_PSEPA              Reviewed;         302 AA.
AC   P22636;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   04-NOV-2008, entry version 44.
DE   RecName: Full=Protocatechuate 4,5-dioxygenase beta chain;
DE            EC=1.13.11.8;
DE   AltName: Full=4,5-PCD;
GN   Name=ligB;
OS   Pseudomonas paucimobilis (Sphingomonas paucimobilis).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingomonas.
OX   NCBI_TaxID=13689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-9.
RC   STRAIN=SYK-6;
RX   MEDLINE=90236935; PubMed=2185230;
RA   Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K.,
RA   Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M.;
RT   "Molecular cloning of the protocatechuate 4,5-dioxygenase genes of
RT   Pseudomonas paucimobilis.";
RL   J. Bacteriol. 172:2704-2709(1990).
CC   -!- FUNCTION: Responsible for the aromatic ring fission of
CC       protocatechuate.
CC   -!- CATALYTIC ACTIVITY: Protocatechuate + O(2) = 4-carboxy-2-
CC       hydroxymuconate semialdehyde.
CC   -!- COFACTOR: Fe(2+) ion.
CC   -!- SUBUNIT: Composed of two subunits (alpha and beta) in a 1:1 ratio.
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DR   EMBL; M34835; AAA17728.1; -; Unassigned_DNA.
DR   PIR; B35271; B35271.
DR   PDB; 1B4U; X-ray; 2.20 A; B/D=1-302.
DR   PDB; 1BOU; X-ray; 2.20 A; B/D=1-302.
DR   PDBsum; 1B4U; -.
DR   PDBsum; 1BOU; -.
DR   GO; GO:0018579; F:protocatechuate 4,5-dioxygenase activity; IEA:EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004183; Xdiol_dOase_3B.
DR   Gene3D; G3DSA:3.40.830.10; Xdiol_dOase_3B; 1.
DR   Pfam; PF02900; LigB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aromatic hydrocarbons catabolism; Dioxygenase;
KW   Direct protein sequencing; Iron; Oxidoreductase.
FT   CHAIN         1    302       Protocatechuate 4,5-dioxygenase beta
FT                                chain.
FT                                /FTId=PRO_0000085101.
FT   STRAND        3     10
FT   HELIX        14     21
FT   TURN         28     30
FT   HELIX        31     44
FT   TURN         47     49
FT   STRAND       52     58
FT   STRAND       62     64
FT   STRAND       69     76
FT   STRAND       78     81
FT   STRAND       87     90
FT   HELIX       100    112
FT   STRAND      118    122
FT   HELIX       127    137
FT   STRAND      144    152
FT   STRAND      155    157
FT   HELIX       162    177
FT   STRAND      179    181
FT   STRAND      184    190
FT   TURN        200    203
FT   HELIX       207    219
FT   HELIX       221    224
FT   HELIX       229    236
FT   HELIX       240    243
FT   HELIX       244    251
FT   STRAND      257    268
FT   STRAND      271    280
FT   HELIX       281    283
FT   STRAND      289    296
SQ   SEQUENCE   302 AA;  33292 MW;  0552B3B0E59702E5 CRC64;
     MARVTTGITS SHIPALGAAI QTGTSDNDYW GPVFKGYQPI RDWIKQPGNM PDVVILVYND
     HASAFDMNII PTFAIGCAET FKPADEGWGP RPVPDVKGHP DLAWHIAQSL ILDEFDMTIM
     NQMDVDHGCT VPLSMIFGEP EEWPCKVIPF PVNVVTYPPP SGKRCFALGD SIRAAVESFP
     EDLNVHVWGT GGMSHQLQGP RAGLINKEFD LNFIDKLISD PEELSKMPHI QYLRESGSEG
     VELVMWLIMR GALPEKVRDL YTFYHIPASN TALGAMILQP EETAGTPLEP RKVMSGHSLA
     QA
//