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UniProtKB/Swiss-Prot entry P21954


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IDHP_YEAST
Primary accession number P21954
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on August 1, 1991 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 79)
Name and origin of the protein
Protein name Isocitrate dehydrogenase [NADP], mitochondrial [Precursor]
Synonyms IDH
EC 1.1.1.42
Oxalosuccinate decarboxylase
NADP(+)-specific ICDH
IDP
Gene name
Name: IDP1
OrderedLocusNames: YDL066W
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 17-36.
PubMed=1989987 [NCBI, ExPASy, EBI, Israel, Japan]
Haselbeck R.J., McAlister-Henn L.;
"Isolation, nucleotide sequence, and disruption of the Saccharomyces cerevisiae gene encoding mitochondrial NADP(H)-specific isocitrate dehydrogenase.";
J. Biol. Chem. 266:2339-2345(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169867 [NCBI, ExPASy, EBI, Israel, Japan]
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
Nature 387:75-78(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M57229; AAA34703.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z74114; CAA98631.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A38610; DCBYIS.
RefSeq NP_010217.1; -.
3D structure databases
HSSP P33198; 1LWD. [HSSP ENTRY / PDB]
ModBase P21954.
Protein-protein interaction databases
DIP DIP:4494N; -.
IntAct P21954; -.
Organism-specific databases
CYGD YDL066w; -.
SGD S000002224; IDP1.
Yeast-GFP YDL066W.
Gene expression databases
ArrayExpress P21954; -.
GermOnline YDL066W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0042645; Cellular component: mitochondrial nucleoid (inferred from direct assay from SGD).
GO:0004450; Molecular function: isocitrate dehydrogenase (NADP+) activity (traceable author statement from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006537; Biological process: glutamate biosynthetic process (inferred from genetic interaction from SGD).
GO:0006102; Biological process: isocitrate metabolic process (traceable author statement from SGD).
QuickGo view.
Family and domain databases
InterPro IPR004790; IsoCit_DHase_NADP-dep_euk.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11822; IDH_NADP_euk; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000108; IDH_NADP; 1.
TIGRFAMs TIGR00127; nadp_idh_euk; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS P21954.
Proteomic databases
PeptideAtlas P21954; -.
Genome annotation databases
Ensembl YDL066W; Saccharomyces cerevisiae. [Contig view]
GeneID 851493; -.
GenomeReviews Z71256_GR; YDL066W.
KEGG sce:YDL066W; -.
NMPDR fig|4932.3.peg.957; -.
Phylogenomic databases
HOGENOM P21954; -.
Other
LinkHub P21954; -.
ProtoNet P21954.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; Mitochondrion; NADP; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
TRANSIT   1    16  16     Mitochondrion. 
CHAIN   17   428  412     Isocitrate dehydrogenase [NADP], mitochondrial. PRO_0000014426
NP_BIND   91    93  3     NADP (By similarity). 
NP_BIND   327   332  6     NADP (By similarity). 
REGION   110   116  7     Substrate binding (By similarity). 
METAL   269   269        Magnesium or manganese (By similarity). 
METAL   292   292        Magnesium or manganese (By similarity). 
BINDING   93    93        Substrate (By similarity). 
BINDING   98    98        NADP (By similarity). 
BINDING   125   125        Substrate (By similarity). 
BINDING   148   148        Substrate (By similarity). 
BINDING   277   277        NADP (By similarity). 
BINDING   345   345        NADP; via amide nitrogen and carbonyl oxygen (By similarity). 
SITE   155   155  1     Critical for catalysis (By similarity). 
SITE   229   229  1     Critical for catalysis (By similarity). 
Sequence information
Length: 428 AA [This is the length of the unprocessed precursor] Molecular weight: 48190 Da [This is the MW of the unprocessed precursor] CRC64: 7F885785B8682416 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSMLSRRLFS TSRLAAFSKI KVKQPVVELD GDEMTRIIWD KIKKKLILPY LDVDLKYYDL 

        70         80         90        100        110        120 
SVESRDATSD KITQDAAEAI KKYGVGIKCA TITPDEARVK EFNLHKMWKS PNGTIRNILG 

       130        140        150        160        170        180 
GTVFREPIVI PRIPRLVPRW EKPIIIGRHA HGDQYKATDT LIPGPGSLEL VYKPSDPTTA 

       190        200        210        220        230        240 
QPQTLKVYDY KGSGVAMAMY NTDESIEGFA HSSFKLAIDK KLNLFLSTKN TILKKYDGRF 

       250        260        270        280        290        300 
KDIFQEVYEA QYKSKFEQLG IHYEHRLIDD MVAQMIKSKG GFIMALKNYD GDVQSDIVAQ 

       310        320        330        340        350        360 
GFGSLGLMTS ILVTPDGKTF ESEAAHGTVT RHYRKYQKGE ETSTNSIASI FAWSRGLLKR 

       370        380        390        400        410        420 
GELDNTPALC KFANILESAT LNTVQQDGIM TKDLALACGN NERSAYVTTE EFLDAVEKRL 


QKEIKSIE 

P21954 in FASTA format

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